Nrf2-mediated protection against UVA radiation in human skin keratinocytes

Tian, Fei; Zhang, Feifei; Lai, Xiangdong; Wang, Limin; Yang, Li; Wang, Xiang; Singh, Govind; Zhong, Julia Li

doi:10.5582/bst.2011.v5.1.23

Cited by 50 publications

(44 citation statements)

References 21 publications

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“…1c). Cell viability loss was 20% and no morphology changes were observed at the UVA irradiation dose of 160 kJ/m 2 ; therefore, this UVA dose was used throughout the experiments unless otherwise stated, which is comparable with previous studies [21,27]. …”

Section: Resultssupporting

confidence: 48%

“…Previously we described that in HaCaT cells, HO-1 expression was increased with Bach1 RNA interference, but downregulated by Nrf2 RNA interference [19,21]. According to these findings, we speculated that AKBA may modulate UVA-induced cellular damage via regulation of Bach1/Nrf2-mediated HO-1 expression.…”

Section: Resultsmentioning

confidence: 77%

“…Immortalized human skin keratinocytes (HaCaT) and mouse embryonic fibroblasts, including wild-type cells (WT) and Keap1 knockout cells (Keap1 -/- ), were cultured in RPMI 1640 medium as previously reported [21,22]. Cells were pretreated with 0.125-2.5 μ M of AKBA for 12 h and were then exposed to UVA irradiation at the indicated doses from 80 to 400 kJ/m 2 [24,25] by using a broad-spectrum UV lamp (UVB was blocked with an appropriate filter provided with the instrument) at the peak wavelength of 365 nm (MUA-165, Beijing Normal University, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…However, transactivation of Nrf2 can be inhibited by another CNC transcription repressor, Bach1 (BTB and CNC homology 1), through binding to ARE sequences in target genes [19,20]. Nrf2 has been found to protect human keratinocytes and mouse dermal fibroblasts from UVA irradiation-mediated damage [21,22]. …”

Section: Introductionmentioning

confidence: 99%

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Nrf2- and Bach1 May Play a Role in the Modulation of Ultraviolet A-Induced Oxidative Stress by Acetyl-11-Keto-β-Boswellic Acid in Skin Keratinocytes

Yang¹,

Zhou²,

et al. 2017

Skin Pharmacol Physiol

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Background: Exposure of human skin to solar ultraviolet A (UVA) irradiation causes severe oxidative stress with damage to various cellular components and concomitant inflammation and carcinogenesis. Objective: The aim of this study is to investigate the protective effect of acetyl-11-keto-β-boswellic acid (AKBA) against UVA radiation on human skin keratinocytes. Methods: HaCaT cells were pretreated with AKBA followed by UVA irradiation. Radiation effects on cell morphology, cell viability, intracellular reactive oxygen species (ROS) levels, and antioxidant enzymes were examined. Results: AKBA reduces UVA irradiation-induced cell viability loss, accompanied by a decreased production of UVA-induced ROS, decreased malondialdehyde, and increased superoxide dismutase expression. In addition, AKBA increased basal and UVA-induced levels of Nrf2 (NF-E2-related factor 2), the redox-sensitive factor, and its target genes NQO1 and heme oxygenase-1 (HO-1), whereas expression of the transcriptional repressor Bach1 (BTB and CNC homology 1) was reduced. Furthermore, the cytoprotective effects of AKBA against UVA-derived oxidative damage were accompanied by modulating expression of inflammatory mediators (i.e., cyclooxygenase-2 and nuclear factor-κB) and NOX1. Conclusions: AKBA protects skin cells from UVA-induced damage by modulating inflammatory mediators and/or ROS production. Therefore, AKBA has potential in the development of skin care products.

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Section: Resultssupporting

confidence: 48%

Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nrf2- and Bach1 May Play a Role in the Modulation of Ultraviolet A-Induced Oxidative Stress by Acetyl-11-Keto-β-Boswellic Acid in Skin Keratinocytes

Yang¹,

Zhou²,

et al. 2017

Skin Pharmacol Physiol

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show abstract

“…Later, Nrf2-driven HO-1 expression was found to protect mouse skin cells from oxidative carcinogenesis (32). Our studies also showed that Nrf2 might protect human skin fibroblasts and keratinocytes from UVA irradiation (23,33). These studies indicated that Nrf2-driven HO-1 expression may play a role in cellular protection.…”

Section: Uva Phototherapymentioning

confidence: 48%