Nuclear localisation and pDNA condensation in non‐viral gene delivery

Collins, Elizabeth; Birchall, James Caradoc; Williams, Julie; Gumbleton, Mark

doi:10.1002/jgm.1015

Cited by 32 publications

(23 citation statements)

References 40 publications

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“…A number of previous reports have shown that electroporation is an effective method for in vitro gene transfer by direct delivery into the cytoplasm (Zabner et al, 1995;Lechardeur et al, 1999;Collins et al, 2007). We also expected elevation of pDNA intracellular uptake by electroporation prior to increase of nuclear transport.…”

Section: Effect Of Combination Of Plga/pei Nanospheres Nls and Electmentioning

confidence: 64%

“…Therefore, such peptides can serve several roles, such as potential nuclear signaling moieties and the formulation of positively charged pDNA complexes (Conary et al, 1996;Aronsohn and Hughes, 1997;Collins et al, 2007). The modestly up-regulation of pEGFP cellular uptake upon the addition of NF-B p50 analog in the present study was due to an increase in complex attachment to the cell membrane by positively charged amino acids.…”

Section: Effect Of Combination Of Plga/pei Nanospheres Nls and Electmentioning

confidence: 71%

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Enhancement of gene transfection into human dendritic cells using cationic PLGA nanospheres with a synthesized nuclear localization signal

Kanazawa

Takashima

Murakoshi

et al. 2009

International Journal of Pharmaceutics

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Section: Effect Of Combination Of Plga/pei Nanospheres Nls and Electmentioning

confidence: 64%

Section: Effect Of Combination Of Plga/pei Nanospheres Nls and Electmentioning

confidence: 71%

Enhancement of gene transfection into human dendritic cells using cationic PLGA nanospheres with a synthesized nuclear localization signal

Kanazawa

Takashima

Murakoshi

et al. 2009

International Journal of Pharmaceutics

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“…Sasaki et al also reported that PEI polyplexes significantly enhanced transgene expression in the liver compared with control mice at 48 h after CCl 4 injection. It was demonstrated that the transfection efficiency of non-viral vectors critically depends on the cell cycle and is enhanced by mitotic activity in the regeneration (Mortimer, 1999;Collins, 2007). Actually, Tada et al reported that partial hepatectomy of mice increased transgene expression of PEI/pDNA complexes in the liver with increased liver weight (Tada, 2006).…”

Section: Discussionmentioning

confidence: 99%

Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride

Yoshioka

Yoshida

Kurosaki

et al. 2009

Journal of Liposome Research

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In order to elucidate the influence of hepatic disease-stage on cationic liposomes-mediated gene delivery, we investigated the cationic liposomes-mediated plasmid DNA delivery with time in murine hepatitis induced by subcutaneous injection of CCl 4 . Liver injury after injection of In conclusion, our findings demonstrate that murine hepatitis induced by CCl 4 can influence cationic liposomes-mediated plasmid DNA delivery. The extent of influences was also affected by lipid contents. These results indicate the necessity of considering the timing and the formulation for gene therapy according to the disease stage.

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“…As seen from Figure Figure 5). Cationic entities such as protamine, 13,18 polylysine, or NLS-containing peptides 19,4 have been widely evaluated for their gene delivery potential and are frequently used to condense and protect plasmid DNA. As seen from the graph, under these conditions, a control cell line MDA-MB-231, expressing basal levels of HER2/3, was not transfected.…”

Section: Her-nls Mediated Uptake Of Dna Into Mda-mb-453 Cells In Vitromentioning

confidence: 99%

Plasmid DNA delivery into MDA-MB-453 cells mediated by recombinant Her-NLS fusion protein

Jeyarajan

Xavier²,

Rao³

et al. 2010

IJN

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A major rate-limiting step in nonviral gene delivery is the entry of nucleic acids across various membrane barriers and eventually into the nucleus where it must be transcribed. Cell-penetrating peptides and proteins are employed to generate formulations that overcome these challenges to facilitate DNA delivery into cells efficiently. However, these are limited by their inability to deliver nucleic acids selectively due to lack of specificity because they deliver to both cancer and normal cells. In this study, through modular design, we generated a recombinant fusion protein designated as Her-nuclear localization sequence (Her-NLS), where heregulin-α . (Her), a targeting moiety, was cloned in frame with cationic NLS peptide to obtain a cell-specific targeting biomolecule for nucleic acid delivery. The heregulin-α 1 isoform possesses the epidermal growth factor-like domain and binds to HER2/3 heterodimers which are overexpressed in certain breast cancers. Purified recombinant Her-NLS fusion protein binds plasmid DNA and specifically transfects MDA-MB-453 cells overexpressing the epidermal growth factor receptors HER2/3 in vitro. The approach described would also permit replacement of heregulin ligand with other targeting moieties that would be suited to cell-specific nucleic acid delivery mediated via receptor-ligand interactions.

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Nuclear localisation and pDNA condensation in non‐viral gene delivery

Cited by 32 publications

References 40 publications

Enhancement of gene transfection into human dendritic cells using cationic PLGA nanospheres with a synthesized nuclear localization signal

Enhancement of gene transfection into human dendritic cells using cationic PLGA nanospheres with a synthesized nuclear localization signal

Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride

Plasmid DNA delivery into MDA-MB-453 cells mediated by recombinant Her-NLS fusion protein

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