Nuclear localization of the zebrafish tight junction protein nagie oko

Bit‐Avragim, Nana; Rohr, Stefan; Rudolph, Franziska; Ven, Peter F.M. van der; Fürst, Dieter O.; Eichhorst, Jenny; Wiesner, Burkhard; Abdelilah‐Seyfried, Salim

doi:10.1002/dvdy.21389

Cited by 7 publications

(10 citation statements)

References 103 publications

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“…This suggests that, depending on the apical polarity protein altered, a number of different actions might occur in the cell nucleus, which will shape the progression and possibly the outcome of cancer. Nok a homolog of PALS1 in zebrafish can localize to the cell nucleus [100]. Par-6 has also been observed in the cell nucleus in association with splicing factor speckles and it interacts with transcriptional activator Tax [101].…”

Section: Relationship Between Apical Polarity and Nuclear Mechanisms mentioning

confidence: 99%

Tissue Polarity-Dependent Control of Mammary Epithelial Homeostasis and Cancer Development: an Epigenetic Perspective

Lelièvre

2010

J Mammary Gland Biol Neoplasia

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The basoapical organization of monolayered epithelia is defined by the presence of hemidesmosomes at the basal cellular pole, where the cell makes contacts with the basement membrane, and tight junctions at the opposite apical pole. In the mammary gland, tight junctions seal cell-cell contacts against the lumen and separate the apical and basolateral cell membranes. This separation is critical to organize intracellular signaling pathways and the cytoskeleton. The study of the impact of the highly organized apical pole, and notably apical polarity regulators (Crb complex, Par complex, and Scrib, Dlg, Lgl proteins) and tight junction proteins on cell phenotype and gene expression has revealed an intricate relationship between apical polarity and the cell nucleus. The goal of this review is to highlight the role of the apical pole of the tissue polarity axis in the epigenetic control of tissue phenotype. The organization of the apical pole and its importance in mammary homeostasis and tumorigenesis will be emphasized before presenting how apical polarity proteins impact gene expression indirectly, by influencing signal transduction and the location of transcription regulators, and directly, by participating in chromatin-associated complexes. The relationship between apical polarity and cell nucleus organizations might explain how apical polarity proteins could switch from nuclear repressors to nuclear promoters of cancerous behavior following alterations in the apical pole. The impact of apical polarity proteins on epigenetic mechanisms of gene expression will be discussed in light of increased evidence supporting a role for apical polarity in the fate of breast neoplasms.

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Section: Relationship Between Apical Polarity and Nuclear Mechanisms mentioning

confidence: 99%

Tissue Polarity-Dependent Control of Mammary Epithelial Homeostasis and Cancer Development: an Epigenetic Perspective

Lelièvre

2010

J Mammary Gland Biol Neoplasia

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“…Although we have not detected its localization in mitotic spindles, Patj localization in the nucleus during selective mitotic phases would be consistent with a role for Crumbs complex proteins in mitotic progression. The zebrafish ortholog of Pals1 nagie oko was shown to shuttle between the cytoplasm and nucleus via its putative nuclear export and localization signals (Bit-Avragim et al, 2008b); similar studies have not been performed in mammalian systems. Nuclear localization of Patj in subsets of mitotic cells, if proven, may provide further support for a nuclear function of Crb polarity complex.…”

Section: Resultsmentioning

confidence: 99%

“…This versatility can increase the usage of a protein. For example, in the zebrafish mutant of Pals1, Nagie Oko, Pals1 was shown to be localized in the nucleus using its two putative conserved nuclear localization signals and association with Par6-aPKC through its ECR1 (Bit-Avragim et al, 2008b). In addition, deletion of Pals1, which is expressed in the apical villi of retinal pigmented epithelium (RPE), induces abnormal formation of RPE, a phenotype not reported in Crb1 mutant, as well as retinal architectural disorganization (Park et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Common and distinctive localization patterns of Crumbs polarity complex proteins in the mammalian eye

Kim

Song

Karnam

et al. 2015

Gene Expression Patterns

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Crumbs polarity complex proteins are essential for cellular and tissue polarity, and for adhesion of epithelial cells. In epithelial tissues deletion of any of three core proteins disrupts localization of the other proteins, indicating structural and functional interdependence among core components. Despite previous studies of function and co-localization that illustrated the properties that these proteins share, it is not known whether an individual component of the complex plays a distinct role in a unique cellular and developmental context. In order to investigate this question, we primarily used confocal imaging to determine the expression and subcellular localization of the core Crumbs polarity complex proteins during ocular development. Here we show that in developing ocular tissues core Crumbs polarity complex proteins, Crb, Pals1 and Patj, generally appear in an overlapping pattern with some exceptions. All three core complex proteins localize to the apical junction of the retinal and lens epithelia. Pals1 is also localized in the Golgi of the retinal cells and Patj localizes to the nuclei of the apically located subset of progenitor cells. These findings suggest that core Crumbs polarity complex proteins exert common and independent functions depending on cellular context.

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“…Proteins were expressed and purified as described [32]. Rabbits were immunized four times with the purified recombinant proteins according to a standard protocol (Biogenes, Berlin, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Rabbits were immunized four times with the purified recombinant proteins according to a standard protocol (Biogenes, Berlin, Germany). Antibodies were purified as described [32]. Immunohistochemistry was performed as described, with an Aceton permeabilization step of 5–10 minutes at −20°C prior to the blocking step [29].…”

Section: Methodsmentioning

confidence: 99%

Xirp Proteins Mark Injured Skeletal Muscle in Zebrafish

et al. 2012

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Myocellular regeneration in vertebrates involves the proliferation of activated progenitor or dedifferentiated myogenic cells that have the potential to replenish lost tissue. In comparison little is known about cellular repair mechanisms within myocellular tissue in response to small injuries caused by biomechanical or cellular stress. Using a microarray analysis for genes upregulated upon myocellular injury, we identified zebrafish Xin-actin-binding repeat-containing protein1 (Xirp1) as a marker for wounded skeletal muscle cells. By combining laser-induced micro-injury with proliferation analyses, we found that Xirp1 and Xirp2a localize to nascent myofibrils within wounded skeletal muscle cells and that the repair of injuries does not involve cell proliferation or Pax7+ cells. Through the use of Xirp1 and Xirp2a as markers, myocellular injury can now be detected, even though functional studies indicate that these proteins are not essential in this process. Previous work in chicken has implicated Xirps in cardiac looping morphogenesis. However, we found that zebrafish cardiac morphogenesis is normal in the absence of Xirp expression, and animals deficient for cardiac Xirp expression are adult viable. Although the functional involvement of Xirps in developmental and repair processes currently remains enigmatic, our findings demonstrate that skeletal muscle harbours a rapid, cell-proliferation-independent response to injury which has now become accessible to detailed molecular and cellular characterizations.

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Nuclear localization of the zebrafish tight junction protein nagie oko

Cited by 7 publications

References 103 publications

Tissue Polarity-Dependent Control of Mammary Epithelial Homeostasis and Cancer Development: an Epigenetic Perspective

Tissue Polarity-Dependent Control of Mammary Epithelial Homeostasis and Cancer Development: an Epigenetic Perspective

Common and distinctive localization patterns of Crumbs polarity complex proteins in the mammalian eye

Xirp Proteins Mark Injured Skeletal Muscle in Zebrafish

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