Nuclear maturation of ovine oocytes in cultured preantral follicles

Tamilmani, G; Rao, Brahmasani Sambasiva; Vagdevi, R.; Amarnath, Dasari; Naik, B.R.; Mutharao, M.; Rao, V. R.

doi:10.1016/j.smallrumres.2005.01.010

Cited by 27 publications

(33 citation statements)

References 25 publications

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“…Notably, only in treatments with VEGF-A 165 did the oocytes reach MII. In goats, the rates of MII oocytes grown in vitro is still low compared with those of in-vivogrown oocytes (60%-70% of MII) obtained from antral follicles (Chauan and Anand 1991); the production of mature oocytes from preantral follicles grown in vitro in domestic animals has only been reported in sheep (Tamilmani et al 2005;Arunakumari et al 2007Arunakumari et al , 2010, pigs (Wu et al 2001) and buffalo (Gupta et al 2008). To date, in goat preantral follicles, a low MII rate has been reported (Magalhães et al 2010;Duarte et al 2010).…”

Section: Discussionmentioning

confidence: 97%

Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

et al. 2011

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The aim of this study was to evaluate the effect of vascular endothelial growth factor-A(165) (VEGF-A(165)) on the in vitro development of goat secondary preantral follicles. Preantral follicles (≥150 μm in diameter) were isolated from the ovaries of adult mixed-breed goats and individually cultured for 18 days in αMEM in the absence (control) or presence of VEGF-A(165) at concentrations of 10 ng/ml (VEGF10) and 100 ng/ml (VEGF100). Analyses of follicular survival, diameter, antrum formation and rate of daily growth were performed every 6 days. At the end of the culture period, morphologically normal oocytes (≥110 μm in diameter) were taken for in vitro maturation (IVM). The results demonstrated that all follicles presented oocytes and granulosa cells that were morphologically normal and after labeling with calcein-AM, high rates of oocyte viability were observed in all treatments. The follicular diameter and the growth rate achieved in the presence of VEGF10 were higher than those of the control. Both treatments with VEGF-A(165) showed higher rates of oocyte recovery for IVM when compared with the control. Moreover, only the addition of VEGF-A(165) permitted oocytes grown in vitro to reach metaphase II. Thus, the addition of VEGF-A(165) to the culture medium improves the development of goat preantral follicles cultured in vitro, allowing the production of mature oocytes.

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Section: Discussionmentioning

confidence: 97%

Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

et al. 2011

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“…However, difference was noticed with LH supplemented at 100 ng/ml. It was reported by earlier studies that the effect of LH on the in vitro culture of preantral follicles depends on the follicular category, the concentration used and the timing of addition of LH to the culture medium (Tamilmani et al, 2005;Silva et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…All media were incubated at 39 o C under a humidified atmosphere of 5% CO 2 in air for 2 h prior to use. Phosphate buffered saline (PBS), collection medium for PFs [HEPES buffered tissue culture medium 199 supplemented with 0.5% bovine serum albumin (BSA), 50 µg/mL gentamicin sulphate, 0.23 mM of sodium pyruvate, 2 mM l-Glutamine and 25 IU/mL heparin], handling medium (collection medium without heparin), stock solutions of EGF and FSH were all prepared as described by Tamilmani et al, (2005). The preparations of thyroxin, GH and IGF-I solutions were made as per Arunakumari et al, (2007Arunakumari et al, ( , 2010.…”

Section: Methodsmentioning

confidence: 99%

Effect of Different Combinations of the Growth Factors and Hormones on In vitro Maturation of Goat Preantral Follicles

Shankaraiah¹,

Swathi²,

Kumari³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Lack of a repeatable culture system for efficient production of competent oocytes from cultured PFs hampers the maximum utilization of oocytes. Several studies have been aimed at producing competent oocytes from cultured PFs of various species: laboratory animals (Wang and Roy, 2004;Hasegawa et al, 2004), farm animals (Cecconi et al, 1999;Wu et al, 2001a, b;Itoh et al, 2002;Tamilmani et al, 2005;Gupta et al, 2007;Arunakumari et al, 2007Arunakumari et al, , 2010, non-human primates (Wandji et al, 1997), as well as humans (Carlsson et al, 2006). However, offspring have only been produced in mice using oocytes from cultured PFs (Eppig and O'Brien, 1996;O'Brien et al, 2003).…”

Section: Introductionmentioning

confidence: 99%