Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

Araújo, Valdevane Rocha; Silva, G. M.; Duarte, A.B.G.; Magalhães, D. M.; Almeida, A. P.; Gonçalves, Raphael Fernando Braga; Bruno, Jamily Bezerra; Silva, T. F. P.; Campello, C.C.; Rodrigues, A. P. R.; Figueiredo, J.R.

doi:10.1007/s00441-011-1251-1

Cited by 46 publications

(32 citation statements)

References 27 publications

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“…These down-regulated target genes are known to be involved in different developmental process and cellular network [59], [60], [61], [62], [63], [64]. However, the VEGFA gene, which is known to play a role in improving neovascularization and vascular permeability close to the developing of goat preantral follicles [65] has shown increased abundance after exosome mediated miRNA transfection of granulosa cells. Taken together, results of the present study demonstrate the potential of extra-cellular miRNAs in follicular fluid in regulation of transcripts, which are involved in various processes associated with follicular development, in surrounding follicular cells.…”

Section: Discussionmentioning

confidence: 99%

Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence

et al. 2013

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Cell-cell communication within the follicle involves many signaling molecules, and this process may be mediated by secretion and uptake of exosomes that contain several bioactive molecules including extra-cellular miRNAs. Follicular fluid and cells from individual follicles of cattle were grouped based on Brilliant Cresyl Blue (BCB) staining of the corresponding oocytes. Both Exoquick precipitation and differential ultracentrifugation were used to separate the exosome and non-exosomal fraction of follicular fluid. Following miRNA isolation from both fractions, the human miRCURY LNA™ Universal RT miRNA PCR array system was used to profile miRNA expression. This analysis found that miRNAs were present in both exosomal and non-exosomal fraction of bovine follicular fluid. We found 25 miRNAs differentially expressed (16 up and 9 down) in exosomes and 30 miRNAs differentially expressed (21 up and 9 down) in non-exosomal fraction of follicular fluid in comparison of BCB- versus BCB+ oocyte groups. Expression of selected miRNAs was detected in theca, granulosa and cumulus oocyte complex. To further explore the potential roles of these follicular fluid derived extra-cellular miRNAs, the potential target genes were predicted, and functional annotation and pathway analysis revealed most of these pathways are known regulators of follicular development and oocyte growth. In order to validate exosome mediated cell-cell communication within follicular microenvironment, we demonstrated uptake of exosomes and resulting increase of endogenous miRNA level and subsequent alteration of mRNA levels in follicular cells in vitro. This study demonstrates for the first time, the presence of exosome or non-exosome mediated transfer of miRNA in the bovine follicular fluid, and oocyte growth dependent variation in extra-cellular miRNA signatures in the follicular environment.

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Section: Discussionmentioning

confidence: 99%

Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence

et al. 2013

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“…Several studies have been developed by our team in an attempt to improve oocyte growth rates, resumption of meiosis, and maturation from in vitro‐cultured caprine preantral follicles, but with limited success (Araújo et al, ; Chaves et al, ). The rates of oocyte maturation are still low even with the addition of hormones and/or growth factors to the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

Brito

Silva

Duarte

et al. 2014

Molecular Reproduction Devel

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This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two-dimensional system (control) or a three-dimensional encapsulated system in 0.25%, 0.5%, or 1% alginate (ALG 0.25, ALG 0.5, and ALG 1, respectively). A higher percentage of morphologically normal follicles developed in ALG 0.5 and ALG 1 than in ALG 0.25 or the control (P < 0.05). The rate of antrum formation, however, was higher in ALG 0.25 than in ALG 0.5 and ALG 1 conditions (P < 0.05), but similar to the control. Follicles cultured in ALG 0.25 had higher growth rates and meiotic resumption than those cultured in ALG 0.5, ALG 1, or the control (P < 0.05). Moreover, follicles cultured in ALG 0.25 had higher levels of estradiol and progesterone than those cultured in ALG 0.5, ALG 1, or the control, as well as higher levels of CYP19A1 and HSD3B mRNA. In conclusion, a three-dimensional system that uses ALG 0.25 fosters the in vitro development of caprine preantral follicles and increases the rate of meiotic resumption.

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“…The concentrations of VEGF and FSH were chosen based on previous studies performed in our laboratory (Araújo et al , 2011). For the 2D culture system, the follicles were cultured in 100 μl drops of medium under mineral oil in Petri dishes (60 × 15 mm; Corning, USA).…”

Section: Methodsmentioning

confidence: 99%

In vitro development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems

Silva

Rossetto

Chaves

et al. 2014

Zygote

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Summary The aim of this study was to evaluate the influence of two-dimensional (2D) and three-dimensional (3D) alginate culture systems on in vitro development of pre-antral caprine follicles. In addition, the influence of the reproductive age of the ovary donor on the in vitro culture success was investigated. Pre-antral follicles from pre-pubertal or adult goats were isolated and cultured directly on a plastic surface (2D) or encapsulated in an alginate-based matrix (3D). After 18 days, the oocytes underwent in vitro maturation (IVM) and in vitro fertilization (IVF) to produce embryos. The 3D system showed higher rates of follicle survival, lower rates of oocyte extrusion, and a greater number of recovered oocytes for IVM and IVF (P < 0.05). Only pre-antral follicles from adult animals produced MII oocytes and embryos. The estradiol concentrations increased from day 2 to day 12 of culture in all groups tested (P < 0.05). Conversely, progesterone concentrations were lower in 3D-cultured follicles than in 2D-cultured follicles, with differences on days 2 and 6 of culture (P < 0.05). We provide compelling evidence that a 2D or 3D alginate in vitro culture system offers a promising approach to achieving full in vitro development of caprine pre-antral follicles to produce mature oocytes that are capable of fertilization and viable embryos.

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Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

Cited by 46 publications

References 27 publications

Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence

Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

In vitro development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems

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