Nuclear microprobe imaging of gallium nitrate in cancer cells

Ortega, Richard; Suda, Asami; Devès, Guillaume

doi:10.1016/s0168-583x(03)01052-8

Cited by 19 publications

(15 citation statements)

References 5 publications

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“…Trace element quantification in cisplatin-resistant cells also revealed a strong increase in the copper content (Ortega et al 1996), which can be linked to the expression of copper transport proteins in cisplatin-resistant cells (Ishida et al 2002). The intracellular distribution of some anti-cancer drugs based on other heteroatoms was also investigated, such as iodine in 4 0 -iodo-4 0 -deoxydoxorubicin (Ortega et al 1997;Ortega 2000), gallium nitrate (Ortega et al 2003) and boron (Bench et al 2003;Endo et al 2006). These experiments revealed the intracellular distribution of these anti-cancer drugs and their interaction with endogenous trace metals.…”

Section: Examples Of Applicationmentioning

confidence: 99%

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega

Devès

Carmona

2009

J. R. Soc. Interface.

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141

131

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The direct detection of biologically relevant metals in single cells and of their speciation is a challenging task that requires sophisticated analytical developments. The aim of this article is to present the recent achievements in the field of cellular chemical element imaging, and direct speciation analysis, using proton and synchrotron radiation X-ray micro-and nanoanalysis. The recent improvements in focusing optics for MeV-accelerated particles and keV X-rays allow application to chemical element analysis in subcellular compartments. The imaging and quantification of trace elements in single cells can be obtained using particle-induced X-ray emission (PIXE). The combination of PIXE with backscattering spectrometry and scanning transmission ion microscopy provides a high accuracy in elemental quantification of cellular organelles. On the other hand, synchrotron radiation X-ray fluorescence provides chemical element imaging with less than 100 nm spatial resolution. Moreover, synchrotron radiation offers the unique capability of spatially resolved chemical speciation using micro-X-ray absorption spectroscopy. The potential of these methods in biomedical investigations will be illustrated with examples of application in the fields of cellular toxicology, and pharmacology, bio-metals and metal-based nano-particles.

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Section: Examples Of Applicationmentioning

confidence: 99%

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega

Devès

Carmona

2009

J. R. Soc. Interface.

Self Cite

141

131

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“…The focus of this work is to develop a series of GPCs that contain gallium (Ga) which is known to have a therapeutic effect in treating bone cancer [36]. Radioactive gallium and stable gallium nitrate are used as diagnostic and therapeutic agents in cancer and disorder of calcium and bone metabolism [37].…”

Section: Introductionmentioning

confidence: 99%

Gallium containing glass polyalkenoate anti-cancerous bone cements: glass characterization and physical properties

Wren

Coughlan

Placek

et al. 2012

J Mater Sci: Mater Med

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A gallium (Ga) glass series (0.48SiO(2)-0.40ZnO-0.12CaO, with 0.08 mol% substitution for ZnO) was developed to formulate a Ga-containing Glass Polyalkenoate Cement (GPC) series. Network connectivity (NC) and X-ray Photoelectron Spectroscopy (XPS) was employed to investigate the role of Ga(3+) in the glass, where it is assumed to act as a network modifier. Ga-GPC series was formulated with E9 and E11 polyacrylic acid (PAA) at 50, 55 and 60 wt% additions. E11 working times (T(w)) ranged from 68 to 96 s (Lcon.) and 106 s for the Ga-GPCs (LGa-1 and LGa-2). Setting times (T(s)) ranged from 104 to 226 s (Lcon.) and 211 s for LGa-1 and LGa-2. Compression (σc) and biaxial flexural (σf) testing were conducted where Lcon. increased from 62 to 68 MPa, LGa-1 from 14 to 42 MPa and LGa-2 from 20 to 47 MPa in σc over 1-30 days. σf testing revealed that Lcon. increased from 29 to 42 MPa, LGa-1 from 7 to 32 MPa and LGa-2 from 12 to 36 MPa over 1-30 days.

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“…used micro‐PIXE for the determination of intracellular distribution of anticancer agents. ( 11–13 ) These drugs contain specific trace elements in their molecular structure, such as platinum, iodine and gallium, suggesting the feasibility of their detection in individual cells using micro‐PIXE analysis. In their analysis, 4′‐iodo‐4′‐deoxy‐doxorubicin, a DNA intercalating agent, was mainly detected in the cell nucleus, indicating that the iodine distribution map corresponds to the nucleus area.…”

Section: Discussionmentioning

confidence: 99%

Direct visualization and quantification of the anticancer agent, cis‐diamminedichloro‐platinum(II), in human lung cancer cells using in‐air microparticle‐induced X‐ray emission analysis

et al. 2008

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The authors designed an elemental analysis system using an ion microbeam combined with a microparticle-induced X-ray emission (micro-PIXE) method for the analysis of biomedical samples in air with a spatial resolution of 1 μ μ μ μm (in-air micro-PIXE system). This system was used to develop an imaging and quantification method for intracellular cis-diamminedichloro-platinum(II) (CDDP) in a human lung cancer cell line. A human lung adenocarcinoma cell line, A549, was cultured and nuclear labeling was carried out by incubating the cells with BrdU. The cells were then exposed to CDDP at concentrations ranging from 1 μ μ μ μmol to 1 mmol, for 30 min to 24 h. After drug treatment, samples were washed and frozen with liquid nitrogen, and freeze-dried for 24 h. Standard samples were made using agar containing several concentrations of CDDP. Experiments using standard samples showed a linear correlation between CDDP concentration and platinum signal strength. No clear platinum signal was detected after exposure to CDDP for 24 h at doses between 1 and 100 μ μ μ μmol. However, significant platinum signals were observed at 1 mmol. When nucleus and cytoplasm visualization was sufficiently clear to efficiently use in-air micro-PIXE, the platinum image quality was considered satisfactory. The detected signals of CDDP were stronger in the nucleus than in the cytoplasm. A timecourse study showed increased CDDP uptake in cells after longer drug exposure periods. The present study demonstrates the application of element analysis using in-air micro-PIXE to biomedical samples. The use of this system enables the high-resolution visualization of intracellular CDDP distribution and measurement of intracellular CDDP concentrations. (Cancer Sci 2008; 99: 901-904)

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Nuclear microprobe imaging of gallium nitrate in cancer cells

Cited by 19 publications

References 5 publications

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Gallium containing glass polyalkenoate anti-cancerous bone cements: glass characterization and physical properties

Direct visualization and quantification of the anticancer agent, cis‐diamminedichloro‐platinum(II), in human lung cancer cells using in‐air microparticle‐induced X‐ray emission analysis

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