Nuclear-organelle interactions: Nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants

Rodermel, Steven R.; Abbott, Marilyn S.; Bogorad, Lawrence

doi:10.1016/0092-8674(88)90226-7

Cited by 251 publications

(204 citation statements)

References 41 publications

Supporting

Mentioning

192

Contrasting

Order By: Relevance

“…This reverse genetic approach has been shown to be a powerful tool for the functional analysis of diverse plant gene products (Rodermel et al, 1988;van der Krol et al, 1988;Mol et al, 1990;Sonnewald et al, 1991;Weigel and Nilsson, 1995). Wild-type (ecotype Bensheim) Arabidopsis plants were transformed with binary vectors carrying fusions of the cauliflower mosaic virus 35S RNA promoter and AtHXKI or AtHXK2 in the sense or antisense orientation.…”

Section: Athxk As a Sugar Sensor In Plantsmentioning

confidence: 99%

Hexokinase as a sugar sensor in higher plants.

et al. 1997

View full text Add to dashboard Cite

show abstract

Section: Athxk As a Sugar Sensor In Plantsmentioning

confidence: 99%

Hexokinase as a sugar sensor in higher plants.

et al. 1997

View full text Add to dashboard Cite

show abstract

“…A transformed line of tobacco (Nicotiana tabacum, L. cv W38) described by Rodermel, Abbott & Bogoras (1988) was used. Seeds were sown in 0·9 L plastic containers and were individually transplanted into 1·5 L square pots approximately 2 weeks after emergence.…”

Section: Plant Materialsmentioning

confidence: 99%

Improved temperature response functions for models of Rubisco-limited photosynthesis

et al. 2001

View full text Add to dashboard Cite

Abbreviations: t, Rubisco specificity factor (dimensionless); G*, CO 2 compensation point in the absence of dark respiration (mmol mol -2 ); DH a , energy of activation (kJ mol -1 ); A, net rate of CO 2 uptake per unit leaf area (mmol m -2 s -1 ); c, scaling constant (dimensionless); C i , intercellular CO 2 concentration (mmol mol -1 ); K c , Michaelis constant for CO 2 (mmol mol -1 ); K o , Michaelis constant for O 2 (mmol mol -1 ); R, molar gas constant (kJ K -1 mol -1 ); R d , Mitochondrial respiration rate in the light (mmol m -2 s -1 ); T k , Leaf absolute temperature (K); v c , carboxylation velocity (mmol m -2 s -1 ), V c,max , maximum RuBP saturated rate of carboxylation (mmol m -2 s -1 ); v o , oxygenation velocity (mmol m -2 s -1 ); V o,max , maximum RuBP saturated rate of oxygenation (mmol m -2 s -1 ); W c , RuBP saturated rate of carboxylation (mmol m -2 s -1 ); PPFD, photosynthetically active photon flux density (mmol m -2 s -1 ).

show abstract

“…The antisense mutants display a wide range of Rubisco contents (from 5-100% of WT levels) that approximately correlate with antisense gene dosage-the higher the gene dosage, the lower the Rubisco leve1 (Rodermel et al, 1988). Because of this variability, the antisense mutants have proven to be an attractive system for quantitative fluxcontrol analyses (Kacser and Porteous, 1987) to assess the control that Rubisco exerts on photosynthesis and plant growth under a variety of short-term and long-term growth conditions (Quick et al, 1991a(Quick et al, , 1991b(Quick et al, , 1992Stitt et al, 1991;Lauerer et al, 1993;Masle et al, 1993).…”

Section: ~ ~ ~mentioning

confidence: 99%

“…The SS proteins are translated as precursors on 80s cytoplasmic ribosomes and then transported posttranslationally into the plastid, where they combine with mature LS proteins in a reaction mediated by chaperonin 60 to form the holoenzyme (reviewed by Keegstra et al, 1989;Ellis and van der Vies, 1991). We have previously generated and characterized transgenic tobacco (Nicotiana tabacum) plants that express RbcS antisense RNAs as a tool to gain insight into regulatory interactions between the nucleus-cytoplasm and chloroplast (Rodermel et al, 1988). The antisense plants have reduced XbcS sense mRNA amounts and corresponding, coordinate reductions in SS and LS protein accumulation.…”

Section: ~ ~ ~mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Photosynthesis during Leaf Development in RbcS Antisense DNA Mutants of Tobacco

Jiang

Rodermel

1995

Plant Physiol.

View full text Add to dashboard Cite

We have previously characterized RbcS antisense DNA mutants of tobacco that have drastic reductions in their content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; S.R. Rodermel, M.S. Abbott, 1. Bogorad [1988] Cell55: 673-681). In this report we examine the impact of Rubisco loss on photosynthesis during tobacco (Nicofiana fabacum) leaf development. Photosynthetic capacities are depressed in the antisense leaves, but the patterns of change in photosynthetic rates during the development of these leaves are similar to those in wild-type plants: after attaining a maximum in young leaves, photosynthetic capacities undergo a prolonged senescence decline in older leaves. The alterations in photosynthetic capacities in both the wild type and mutant are closely correlated with changes in Rubisco activity and content. During wild-type leaf development, Rubisco accumulation is regulated by coordinate changes in RbcS and rbcl transcript accumulation, whereas in the antisense leaves, Rubisco content is a function of RbcS, but not rbcL, transcript abundance. This indicates that large subunit protein production is controlled posttranscriptionally in the mutants. The antisense leaves accumulate near-normal levels of chlorophyll and representative photosynthetic proteins throughout development, suggesting that photosynthetic gene expression is not feedback regulated by Rubisco abundance. Considered together, the data in this paper indicate that leaf developmental programs are generally insensitive to sharp reductions in Rubisco content and emphasize the metabolic plasticity of plant cells in

show abstract

Nuclear-organelle interactions: Nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants

Cited by 251 publications

References 41 publications

Hexokinase as a sugar sensor in higher plants.

Hexokinase as a sugar sensor in higher plants.

Improved temperature response functions for models of Rubisco-limited photosynthesis

Regulation of Photosynthesis during Leaf Development in RbcS Antisense DNA Mutants of Tobacco

Contact Info

Product

Resources

About