Nuclease-propelled target dual-recycling amplification strategy integrated with cascaded sensitization effect of ZnO/CuInS2/Ag2Se photoactive structures for lab-on-paper photoelectrochemical microRNA bioassay

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

“…patterned the paper by wax printing and then designed a hollow channel layered structure by cutting and folding, realizing the control of sample liquid (Figure 5Ca–c). [ 98 ]…”

“…For example, Hu et al patterned the paper by wax printing and then designed a hollow channel layered structure by cutting and folding, realizing the control of sample liquid (Figure 5Ca-c). [98] The application of paper-based devices still faces the challenges of having sensory barriers to microfluidic and poor signal visibility. As a result, paper-based microfluidics are still at a low level of accuracy for qualitative detection.…”

“…Hu et al presented a paper-based electrochemical biosensor for miRNA-141 detection. [98] A ZnO/CuInS 2 /Ag 2 Se photosensitive structure with DNA probe and nuclease was modified on a multilayer paper-based material with hollow channels, and the ultrasensitive detection of miRNA-141 was achieved by the dual amplification effect of enzyme-assisted target double-loop and cascade photosensitive structure, providing a paradigm for the development of high-performance paper-based chips.…”

Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

“…In order to continually improve the sensitivity of bioanalytical methods, signal amplification strategies are applied extensively in the designing of various sensors, among which target recycling amplification is the most explored one owing to the associated convenience in designing and operation. 24 Various endonucleases, 25,26 exonucleases, 27,28 nucleases, 29 and metal ion-dependent DNAzymes 30,31 are used as cleavage enzymes in the amplification analysis to enhance the signal/target ratio. RecJf exonuclease is a single-stranded DNA (ssDNA)-specific exonuclease that catalyzes the removal of deoxyribonucleoside monophosphates from the DNA along the 5′ → 3′ direction.…”

Efficient sensitization of CdTe QDs towards PTCDA for sensitive photoelectrochemical Hg2+ assay

“…19 Hu's group reported a paper-based PEC biosensing device for microRNA-141 detection by combining the cascaded sensitized structure of ZnO/ CuInS 2 /Ag 2 Se with a nuclease-propelled target dual-recycling amplication strategy. 20 Encouragingly, sensitization structure coupling the narrow bandgap semiconductor with the wide bandgap material can decidedly upgrade the photocurrent or photovoltage signal by enhancing light absorption and elevating carrier separation.…”