Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter

Brignon, P.; Lepetit, Marc; Gigot, Claude; Chaubet, Nicole

doi:10.1007/bf00028973

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…The interpretation of these observations is supported by flow cytometry analyses [9,19]. At much higher concentrations (100 mM) these effects are not observed [4,22], probably due to severe inhibition of cell metabolism, reflected in the known drop in cell viability [9]. The rate of H3.1 mRNA decay measured with dactinomycin for cultures arrested by hydroxyurea (Fig.…”

Section: Discussionsupporting

confidence: 55%

Histone H3 transcript stability in alfalfa

1995

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The stability of histone H3 transcripts in alfalfa for replication-dependent and -independent gene variants was measured by northern analysis under conditions of inhibition of transcription and/or translation. Replication-dependent histone H3.1 transcripts were about three-fold less stable than the equally polyadenylated mRNA for replacement variant H3.2 histone. In actively growing suspension cultures treated with dactinomycin half-lives of 2 and 7 h were observed for H3.1 and H3.2 mRNAs, respectively. mRNA stabilities were also measured indirectly by histone protein synthesis. The translation inhibitor cycloheximide strongly increased mRNA levels for both histone H3 variants. The dependence of histone mRNA turnover on translation in animals also appears to exist in plants. The combination of inhibition of transcription and translation by dactinomycin and cycloheximide was used in an indirect assessment of H3 mRNA stability throughout the cell cycle in partially synchronized and cycle-arrested cultures. Destabilization of replication-dependent histone H3.1 mRNA was detected in non-S phase cells.

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Section: Discussionsupporting

confidence: 55%

Histone H3 transcript stability in alfalfa

1995

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“…Brown et al, 1988). In the green lineage, this approach has been used only for a few genes, including the maize Shrunken gene (Frommer and Starlinger, 1988), the maize histone H3 gene (Brignon et al, 1993), and the Arabidopsis Adh gene (Vega-Palas and Ferl, 1995). Information on plant chromatin structure was obtained mainly by DNaseI assays (e.g.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chromatin Structure in the Control Regions of the Chlamydomonas HSP70A and RBCS2 Genes

Lodha

Schroda

2005

Plant Mol Biol

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We have used DNaseI and micrococcal nuclease sensitivity assays to determine the chromatin structures in the control regions of the Chlamydomonas reinhardtii HSP70A and RBCS2 genes. Both genes appear to be organized into nucleosome arrays, which exhibit shorter nucleosome repeat lengths than bulk chromatin. In HSP70A we have identified up to four confined DNaseI hypersensitive sites, three of them localize to the promoter region, a fourth one to the fourth intron. Three hypersensitive sites map close to putative heat shock elements, one close to a CCAAT-box. All hypersensitive sites are located to internucleosomal linkers. Alternative nucleosome positions at half-nucleosomal phasing were constitutively detected in the HSP70A promoter region, indicating local chromatin remodelling. Upon heat shock, dramatic changes in the nucleosome structure of HSP70A were detected that particularly affected the promoter, but also a region within the fourth intron. In contrast, light induction entailed no change in HSP70A chromatin. In the RBCS2 control region we identified a strong DNaseI hypersensitive site that maps close to a CCAAT-box. This site forms the boundary of a nucleosome array with a region of approximately 700 bp apparently devoid of nucleosomes. This study demonstrates that chromatin structure may be determined readily at fairly high resolution in Chlamydomonas, suggesting this organism as a well-suited model for studying the role of chromatin structure on gene expression in photosynthetic eukaryotes.

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