Nucleic acids as growth rate indicators for early developmental stages of Calanus pacificus Brodsky

Ota, Allan Y.; Landry, Michael R.

doi:10.1016/0022-0981(84)90009-1

Cited by 50 publications

(33 citation statements)

References 19 publications

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“…The effects of temperature and food on RNA:DNA in this study were similar to the results of Ota & Landry (1984), who found that RNA:DNA ratios of Calanus pacificus were reduced at lower food concentrations and higher temperatures. The effects of temperature and food on protein:DNA and RNA:protein ratios were less clear; these ratios were higher in the high food treatment and showed inverse trends with temperature, but these differences were significant in fewer than half of the stages investigated.…”

Section: Rna As a Growth Rate Indexsupporting

confidence: 90%

“…Two studies have addressed the effect of temperature on nucleic acids of copepods. Ota & Landry (1984) found that RNA:DNA ratios of Calanus pacificus were higher at 8°C than at 15°C, but they did not attempt to quantify the effect of temperature on RNA and its relation to growth rates. Saiz et al (1998) reported that temperature increased the slope of the linear relationship between egg production and RNA:DNA, and they called for consideration of 3 or 4 temperatures in order to refine the technique.…”

Section: Introductionmentioning

confidence: 99%

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Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

Wagner¹,

Campbell²,

Boudreau³

et al. 2001

Mar. Ecol. Prog. Ser.

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We examined the effects of food concentration and temperature on nucleic acids and protein content of Calanus finmarchicus in order to evaluate the use of RNA as a growth rate index for this species. We measured RNA, DNA, and protein content of copepods reared from egg to adult stage in 5 combinations of food and temperature conditions (25 to 500 µg C l -1 , 4 to 12°C). At 8°C, DNA, RNA and protein content and RNA:DNA differed among food treatments during Stages N6 through to adult female. Protein:DNA ratios and RNA:protein ratios were significantly different among food levels for only 3 of the 8 stages examined. At excess food, DNA, RNA, and protein content and RNA:DNA ratios were inversely related to temperature for most stages from C1 onward, but the effect of temperature was relatively small over the range of temperatures investigated. The RNA:DNA and protein:DNA ratios increased with developmental stage whereas the RNA:protein ratio and growth rates (measured in terms of protein, nitrogen, DNA, and carbon content) declined with increasing stage. Although the relationship of RNA:DNA to growth rates was stage-specific, the two were related when standardized for temperature and developmental stage. RNA:protein ratios were directly related to growth rates regardless of stage, and the slope of the relationship increased with increasing temperature in a nonlinear fashion. Our results emphasize the importance of temperature and developmental stage for the relationship of growth rates to RNA concentration and RNA:DNA ratios. We propose 2 ways to estimate in situ growth rates of C. finmarchicus from RNA:DNA or RNA:protein ratios and environmental temperature.

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Section: Rna As a Growth Rate Indexsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

Wagner¹,

Campbell²,

Boudreau³

et al. 2001

Mar. Ecol. Prog. Ser.

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show abstract

“…An increase in the ratio with age or length of the larvae may be effected by cells growing without the DNA content increasing (hypertrophy), which is specific to locomotory muscle DNA (Goss 1966). In future studies this could be further evaluated by determining the C/DNA ratios (Anger and Hirche 1990) or by measuring gg dry weight gg-1 DNA as an index of cell size (Ota and Landry 1984). The increase in RNA/DNA ratios with an increase in the length of the larvae in the group deprived of food for 6 to 9 d is probably due to the fact that the starvation potential is longer in older (larger) larvae.…”

Section: Discussionmentioning

confidence: 99%

The effect of food availability, age or size on the RNA/DNA ratio of individually measured herring larvae: laboratory calibration

1994

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Abstract. RNA/DNA ratios in individual herring (Clupea harengus) larvae (collected from Kiel Bay, Baltic Sea, in 1989) were measured and proved suitable for determining nutritional status. Significant differences between fed and starving larvae appeared after 3 to 4 d of food deprivation in larvae older than 10 d after hatching. The RNA/ DNA ratio showed an increase with age or length of the larvae and was less pronounced in starving larvae compared to fed larvae. The individual variability of RNA/ DNA ratios in relation to larval length of fed larvae and of larvae deprived of food for intervals of 6 to 9 d is presented. Based on the length dependency and the individual variability found within the RNA/DNA ratios, a laboratory calibration is given to determine whether a larva caught in the field has been starving or not. An example for a field application is shown.

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“…Mar Ecol Prog Ser 262: [163][164][165][166][167][168][169][170][171][172] 2003 tested the applicability of nucleic acid analysis for growth rate assessment using RNA concentrations (Dagg & Littlepage 1972, Ota & Landry 1984, Båmstedt & Skjodal 1980 or RNA:DNA values (Wagner et al 1998(Wagner et al , 2001) of mono-or multispecies zooplankton samples. Although most of the early studies focused on using RNA:DNA ratios as an index of nutritional condition (Wagner et al 1998), a calibration of zooplankton growth rates against nucleic acid content has also been attempted, and linear relationships have been found between growth rate and RNA content (and/or RNA:DNA ratio) for various species of marine copepods (Nakata et al 1994, Saiz et al 1998, Wagner et al 2001) and freshwater cladocerans (Vrede et al 2002).…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Relationships between nucleic acid levels and egg production rates in Acartia bifilosa: implications for growth assessment of copepods in situ

Gorokhova¹

2003

Mar. Ecol. Prog. Ser.

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To evaluate the applicability of RNA-based indices in copepod growth assessment, concentrations of nucleic acids in Acartia bifilosa were calibrated against growth rates estimated via egg production experiments, and the relationships between levels of RNA, DNA, and the RNA:DNA ratio and growth rates were examined. Furthermore, to investigate effects of temperature and food availability on the relationships between weight-specific fecundity and nucleic acid levels, incubations were carried out at 9 and 16°C, each at 3 food concentrations. There were positive relationships between nucleic acid concentrations and their ratios and weight-specific egg production rates. Overall, RNA concentration was the best predictor of specific growth rate. No correlations between either of the measured variables and female body size were observed. When egg production was elevated by manipulating the feeding regime, RNA concentration and the RNA:DNA ratio increased in concert. Neither growth nor RNA indices were significantly affected by temperature, while a significant increase in DNA concentrations was observed at high food levels and low temperatures. The lack of temperature dependence in RNA-growth relationships allows their direct application for in situ growth estimates in summer populations of A. bifilosa in the northern Baltic Proper.KEY WORDS: Nucleic acid concentrations · RNA:DNA · Growth rate · Egg production · Weight-specific fecundity · Baltic · Acartia bifilosa Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 262: [163][164][165][166][167][168][169][170][171][172] 2003 tested the applicability of nucleic acid analysis for growth rate assessment using RNA concentrations (Dagg & Littlepage 1972, Ota & Landry 1984, Båmstedt & Skjodal 1980 or RNA:DNA values (Wagner et al. 1998(Wagner et al. , 2001) of mono-or multispecies zooplankton samples. Although most of the early studies focused on using RNA:DNA ratios as an index of nutritional condition (Wagner et al. 1998), a calibration of zooplankton growth rates against nucleic acid content has also been attempted, and linear relationships have been found between growth rate and RNA content (and/or RNA:DNA ratio) for various species of marine copepods (Nakata et al. 1994, Saiz et al. 1998, Wagner et al. 2001) and freshwater cladocerans (Vrede et al. 2002). In some cases, however, the relationship between RNA content and growth rate has been found to lack sufficient predictability (Dagg & Littlepage 1972, Ota & Landry 1984. Of great interest, therefore, are the 'rules' used to estimate growth from nucleic acid measurements, and these need to be defined. For example, it has been shown that temperature influences the relationship between RNA and growth in copepods (Saiz et al. 1998, Wagner et al. 2001) and lobsters (Juinio et al. 1992), while a correlation was found between body size and the RNA:protein ratio in squid (Moltschaniwskyj & Jackson 2000), and between developmental stage and RNA-based indices in copepods (Wa...

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Nucleic acids as growth rate indicators for early developmental stages of Calanus pacificus Brodsky

Cited by 50 publications

References 19 publications

Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

The effect of food availability, age or size on the RNA/DNA ratio of individually measured herring larvae: laboratory calibration

Relationships between nucleic acid levels and egg production rates in Acartia bifilosa: implications for growth assessment of copepods in situ

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