Nucleo-cytoplasmic ratio in ageing skeletal muscle

Manta, Panagiota; Vassilopoulos, Dimitris; Spengos, M

doi:10.1007/bf00383854

Cited by 26 publications

(35 citation statements)

References 5 publications

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“…Several cryosection studies of ageing human muscle did not observe decrease in myonuclear profiles in ageing, although the extent of fibre atrophy was not reported (Manta et al, 1987;Renault et al, 2002). By contrast, our results on significantly atrophied old muscle reveal a decline in nuclei/unit length.…”

Section: Fibre Nuclear Number Declines During Ageingcontrasting

confidence: 79%

“…Studies of fibre atrophy conflict on whether domain size is maintained or not (Allen et al, 1997;Allen et al, 1996;Kasper and Xun, 1996;Manta et al, 1987;Mitchell and Pavlath, 2001;Roy et al, 1999;Wada et al, 2002). One reason for confusion might be that cytoplasmic volume/nucleus differs between fibres of different type and increases with fibre size (Bruusgaard et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy

Brack

Bildsoe

Hughes

2005

Journal of Cell Science

259

224

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Skeletal muscle fibres are multinucleate syncitial cells that change size during adult life depending on functional demand. The relative contribution of change in nuclear number and/or cell growth to fibre size change is unclear. We report that nuclei/unit length decreases in larger fibres during skeletal muscle ageing. This leads to an increased size of nuclear domain (quantity of cytoplasm/number of nuclei within that cytoplasm). Initially, larger fibres have more satellite cells than small fibres, but this advantage is lost as satellite cells decline with age. These changes are accompanied by an overall decline in fibre size, returning domain size to the normal range. Exacerbated loss of fibre nuclei per unit length during ageing of myoD-null mice provides the first experimental support for the hypothesis that a satellite cell defect causes inadequate nuclear replacement. We propose a model in which a decline in satellite cell function and/or number during ageing leads to a loss of nuclei from large fibres and an associated domain size increase that triggers cytoplasmic atrophy through the normal cell-size-regulating machinery.

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Section: Fibre Nuclear Number Declines During Ageingcontrasting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy

Brack

Bildsoe

Hughes

2005

Journal of Cell Science

259

224

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“…Most studies on MND during animal growth in a single species found no difference in nuclear length with body mass (Galavazi and Szirmai, 1971;Manta et al, 1987;Matthew and Moore, 1987;Rosser et al, 2002). Rosser et al found that throughout chicken development, from neonatal to adult, myonuclei had very similar mean lengths (Rosser et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Growth patterns and nuclear distribution in white muscle fibers from black sea bass, Centropristis striata: evidence for the influence of diffusion

Priester

Morton

Kinsey

et al. 2011

Journal of Experimental Biology

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SUMMARYThis study investigated the influence of fiber size on the distribution of nuclei and fiber growth patterns in white muscle of black sea bass, Centropristis striata, ranging in body mass from 0.45 to 4840g. Nuclei were counted in 1m optical sections using confocal microscopy of DAPI-and Acridine-Orange-stained muscle fibers. Mean fiber diameter increased from 36±0.87m in the 0.45g fish to 280±5.47m in the 1885g fish. Growth beyond 2000g triggered the recruitment of smaller fibers, thus significantly reducing mean fiber diameter. Nuclei in the smaller fibers were exclusively subsarcolemmal (SS), whereas in larger fibers nuclei were more numerous and included intermyofibrillar (IM) nuclei. There was a significant effect of body mass on nuclear domain size (F118.71, d.f.3, P<0.0001), which increased to a maximum in fish of medium size (282-1885g) and then decreased in large fish (>2000g). Although an increase in the number of nuclei during fiber growth can help preserve the myonuclear domain, the appearance of IM nuclei during hypertrophic growth seems to be aimed at maintaining short effective diffusion distances for nuclear substrates and products. If only SS nuclei were present throughout growth, the diffusion distance would increase in proportion to the radius of the fibers. These observations are consistent with the hypothesis that changes in nuclear distribution and fiber growth patterns are mechanisms for avoiding diffusion limitation during animal growth.

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“…Myonuclear counts from serial transverse sections of human muscle have shown an increased (Kadi et al, 2004a,b) or constant (Manta et al, 1987) myonuclear number during aging. However, the altered shape and elongation of myonuclei in old age observed in this study, as well as in previous experimental animal studies (Brack et al, 2005;Bruusgaard et al, 2006), may give rise to over-counting myonuclei from cross-sections in old age (Brack et al, 2005).…”

Section: Myonuclear Number and Mnd Sizementioning

confidence: 99%

“…A relationship between the number of myonuclei and fibre size has been reported in trained young (Kadi et al, 1999) and old (Manta et al, 1987;Hikida et al, 2000) individuals, but not in untrained elderly subjects (Hikida et al, 1998), reflecting an aging-and ⁄ or atrophy-related deterioration of this relationship (Ohira et al, 1999). In humans, there are a few reports in the literature on the effects of aging on myonuclear density, describing a constant (Vassilopoulos et al, 1977) or decreased (Manta et al, 1987) cytoplasmic density in old age. In addition, an increased myonuclear density has been observed in elderly men and women, but the study did not correct for muscle fibre size (Kadi et al, 2004a,b).…”

Section: Introductionmentioning

confidence: 99%

Effects of aging and gender on the spatial organization of nuclei in single human skeletal muscle cells

et al. 2010

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SummaryThe skeletal muscle fibre is a syncitium where each myonucleus regulates the gene products in a finite volume of the cytoplasm, i.e., the myonuclear domain (MND). We analysed aging-and gender-related effects on myonuclei organization and the MND size in single muscle fibres from six young (21-31 years) and nine old men (72-96 years), and from six young (24-32 years) and nine old women (65-96 years), using a novel image analysis algorithm applied to confocal images. Muscle fibres were classified according to myosin heavy chain (MyHC) isoform expression. Our image analysis algorithm was effective in determining the spatial organization of myonuclei and the distribution of individual MNDs along the single fibre segments. Significant linear relations were observed between MND size and fibre size, irrespective age, gender and MyHC isoform expression. The spatial organization of individual myonuclei, calculated as the distribution of nearest neighbour distances in 3D, and MND size were affected in old age, but changes were dependent on MyHC isoform expression. In type I muscle fibres, average NN-values were lower and showed an increased variability in old age, reflecting an aggregation of myonuclei in old age. Average MND size did not change in old age, but there was an increased MND size variability. In type IIa fibres, average NN-values and MND sizes were lower in old age, reflecting the smaller size of these muscle fibres in old age. It is suggested that these changes have a significant impact on protein synthesis and degradation during the aging process.

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Nucleo-cytoplasmic ratio in ageing skeletal muscle

Cited by 26 publications

References 5 publications

Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy

Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy

Growth patterns and nuclear distribution in white muscle fibers from black sea bass, Centropristis striata: evidence for the influence of diffusion

Effects of aging and gender on the spatial organization of nuclei in single human skeletal muscle cells

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