Nucleotide sequence analysis of a homologue of herpes simplex virus type 1 gene US9 found in the genome of simian herpes B virus

Abstract

The 10K gene of simian herpes B virus (SHBV) has been located and the nucleotide sequence determined. Its relationship to homologous genes in other herpesviruses has been examined. The SHBV 10K gene exhibits a closer relationship to its homologue in HSV-1 than to those in the other viruses studied. Nucleotide sequence identity of 61% was found between the HSVl and SHBV 10K genes, and 57% identity was found between the corresponding predicted protein Sequences. A comparison of the amino acid sequences of the herpesvirus 1 OK proteins has revealed a number of conserved features. These are examined in relation to possible functions of the 1 OK proteins. Implications for evolutionary relationships between SHBV and other herpesviruses are discussed.

Sequence and Genetic Arrangement of the USRegion of the Monkey B Virus (Cercopithecine Herpesvirus 1) Genome and Comparison with the USRegions of Other Primate Herpesviruses

“…When transmitted to nonmacaque primates, BV produces severe infections which usually involve the central nervous system and are frequently fatal (31,37,38). The molecular basis for the extreme neuropathology of BV in nonmacaque species is an intriguing question that remains unanswered.While sequences for a number of genes of BV have been reported (3,9,23,34,35,37), genomic characterization of BV has largely been limited to restriction analysis and gene mapping by hybridization with HSV gene probes (10,16,17). Such studies have suggested that, for the most part, the BV genome is colinear with that of HSV.…”

“…While sequences for a number of genes of BV have been reported (3,9,23,34,35,37), genomic characterization of BV has largely been limited to restriction analysis and gene mapping by hybridization with HSV gene probes (10,16,17). Such studies have suggested that, for the most part, the BV genome is colinear with that of HSV.…”

Complete Sequence and Comparative Analysis of the Genome of Herpes B Virus ( Cercopithecine Herpesvirus 1 ) from a Rhesus Monkey

“…Sequence analysis of the partial U S regions of B virus and simian agent 8 virus demonstrated that human and primate viruses are colinear in this genomic segment (51). However, only a limited number of B virus gene sequences from the U L region have been published (3,36,62,63), and nothing has been reported about the structure and the gene content of the repeat genomic elements.The size of the B virus genome was estimated previously as 165 kb (26) and 162.5 kb (40), which is significantly larger than the HSV-1 and HSV-2 genomes (152 and 155 kb, respectively). Extra DNA might contain additional genes that are not present in human viruses and may provide insight about B virus pathogenicity in foreign hosts.…”

“…Sequence analysis of the partial U S regions of B virus and simian agent 8 virus demonstrated that human and primate viruses are colinear in this genomic segment (51). However, only a limited number of B virus gene sequences from the U L region have been published (3,36,62,63), and nothing has been reported about the structure and the gene content of the repeat genomic elements.…”

Nucleotide sequence analysis of genes encoding glycoproteins D and J in simian herpes B virus

“…SHBV DNA used in this work was from the strain designated prototypic B virus (Wall et al, 1989), and was prepared as described previously (Killeen et al, 1992). A DNA fragment containing the HSV-1 gD gene was used to probe the SHBV genome by using Southern blotting.…”