Although both beta-and gammaherpesviruses indigenous to great-ape species have been isolated, to date all alphaherpesviruses isolated from apes have proven to be human viruses [herpes simplex virus types 1 (HSV1) and 2 (HSV2) or varicella-zoster virus]. If the alphaherpesviruses have co-evolved with their host species, some if not all ape species should harbour their own alphaherpesviruses. Here, the isolation and characterization of an alphaherpesvirus from a chimpanzee (ChHV) are described. Sequencing of a number of genes throughout the ChHV genome indicates that it is collinear with that of HSV. Phylogenetic analyses place ChHV in a clade with HSV1 and HSV2, the alphaherpesviruses of Old World monkeys comprising a separate clade. Analysis of reactivity patterns of HSV2-immune human sera and ChHV-immune chimpanzee sera by competition ELISA support this relationship. Phylogenetic analyses also place ChHV rather than HSV1 as the closest relative of HSV2.
The sequence of the unique short (U S ) region of monkey B virus (BV) was determined. The 13 genes identified are arranged in the same order and orientation as in herpes simplex virus (HSV). These results demonstrate that the BV U S region is entirely colinear with that of HSV type 1 (HSV-1), HSV-2, and simian agent 8 virus.Cercopithecine herpesvirus 1 (monkey B virus [BV]) is a member of the alphaherpesvirus subfamily indigenous in Asiatic macaque monkeys. The natural history of BV in macaques is similar to that of the human herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) in humans, of simian agent 8 virus (SA8) in green monkeys, and of Herpesvirus papio 2 (HVP-2) in baboons (11,12,38). When transmitted to nonmacaque primates, BV produces severe infections which usually involve the central nervous system and are frequently fatal (31,37,38). The molecular basis for the extreme neuropathology of BV in nonmacaque species is an intriguing question that remains unanswered.While sequences for a number of genes of BV have been reported (3,9,23,34,35,37), genomic characterization of BV has largely been limited to restriction analysis and gene mapping by hybridization with HSV gene probes (10,16,17). Such studies have suggested that, for the most part, the BV genome is colinear with that of HSV. However, it has been reported that the unique short (U S ) region of the BV genome may not be colinear with that of HSV (16). Here we present the sequence of the U S region of the BV genome and its genetic layout relative to that of HSV and SA8.The DNA sequence of the BV rhesus genotype (BVrh) strain E2490 (35) U S region was determined by a combination of cloning restriction fragments of the genome and PCR amplification of small gaps in the sequence with genomic BV DNA as a template. The BVrh U S region (GenBank accession no. AB 077432) is 14,447 nucleotides long, slightly longer than the 12,980 bp reported for HSV-1 strain 17 (26) but close to the 14,329-bp HSV-2 U S region (6). The BVrh U S sequence presented here does not include sequences aligning with the N-terminal 2 codons of the US12 open reading frame (ORF). The overall GϩC content of the BVrh U S region is 73.2%, close to the 75% GϩC estimated for the entire BV genome (19). The coding sequences in the U S region have a combined GϩC content of 74.4%, a value somewhat higher than that for the noncoding intergenic regions (68.9%). BV exhibits a strong bias toward use of codons with G or C in the third position (89.6%) and Arg and Leu codons with C in the first position (93.4%). While this GC bias is strong, it is not as extreme as in the SA8 U S region (76.8% GϩC overall, 92.9% GC in the third position, 97.2% C in the first position of the Arg and Leu codons [13]).Analysis of the BVrh U S sequence for ORFs, homology of predicted amino acid sequences with HSV polypeptides, promoter and transcriptional initiation sites, and mRNA termination sites [consensus poly(A) signals associated with mRNA termination motifs] indicates that the genetic layout of the BVrh U S regio...
The complete DNA sequence of the unique long (U(L)) region of monkey B virus (BV) was determined. Based on sequence homology and the presence of transcriptional control element motifs, homologues of every open reading frame present in the U(L) region of the Human herpesvirus 1 (herpes simplex virus 1, HSV-1) and Human herpesvirus 2 (herpes simplex virus 2, HSV-2) genomes were identified in BV. The BV genes are arranged in the same order and orientation as in HSV. These results demonstrate that the BV U(L) region is entirely co-linear with that of HSV-1 and HSV-2.
In laboratory animal facilities, monkeys and pigs are used for animal experiments, but the details of hepatitis E virus (HEV) infection in these animals
The only genome sequence for monkey B virus (BV; species Macacine herpesvirus 1) is that of an attenuated vaccine strain originally isolated from a rhesus monkey (BVrh). Here we report the genome sequence of a virulent BV strain isolated from a cynomolgus macaque (BVcy). The overall genome organization is the same, although sequence differences exist. The greatest sequence divergence is located in non-coding areas of the long and short repeat regions. Like BVrh, BVcy has duplicated Ori elements and lacks an ORF corresponding to the μ34.5 gene of herpes simplex virus. Nine of ten miRNAs and the majority of ORFs are conserved between BVrh and BVcy. The most divergent genes are several membrane-associated proteins and those encoding immediate early proteins.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.