Nucleotide sequence analysis of Aleutian mink disease parvovirus shows that multiple virus types are present in infected mink

Gottschalck, Elisabeth; Alexandersen, Søren; Cohn, Anders; Poulsen, Lene A.; Bloom, Marshall E.; Aasted, Bent

doi:10.1128/jvi.65.8.4378-4386.1991

Cited by 48 publications

(36 citation statements)

References 45 publications

(94 reference statements)

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“…Although there is only 52% similarity at the amino acid level between VP1/2 from CPV and AMDV [1, 2, 16], it is quite likely that AMDV will pack into a structure similar to that of CPV, containing four loops exposed to the surface of the virion structure. This is based on the fact that the published (hyper)variable regions of different AMDV subtypes [16, 17], when aligned with the published CPV sequence [2], all fall into or close to the published CPV loop structures 1–4, indicating the possible generation of immune‐escape AMDV mutants. In particular, AMDV loop 2 was found to be quite variable [17].…”

Section: Introductionmentioning

confidence: 99%

“…This is based on the fact that the published (hyper)variable regions of different AMDV subtypes [16, 17], when aligned with the published CPV sequence [2], all fall into or close to the published CPV loop structures 1–4, indicating the possible generation of immune‐escape AMDV mutants. In particular, AMDV loop 2 was found to be quite variable [17]. Potential AMDV B‐cell epitope structures are expected to be present in or nearby such loop structures.…”

Section: Introductionmentioning

confidence: 99%

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Epitope Mapping of Aleutian Mink Disease Parvovirus Virion Protein VP1 and 2

Costello¹,

Steenfos²,

Jensen³

et al. 1999

Scand J Immunol

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Six overlapping fragments of the Aleutian Mink Disease parvoVirus (AMDV) virion protein VP1 and 2 (VP1/2) gene were inserted into the expression vector pMAL-c2. Four of the clones carried large overlapping fragments covering the entire VP1/2 gene. The remaining two clones covered specifically chosen regions within the VP1/2 gene. Using a Western blotting detection system, sera from AMDV-infected mink were tested against the recombinant polypeptides. These studies showed reactions primarily directed against the two AMDV polypeptides ranging from amino acids 297 to 518. Weaker reactions against other regions of the VP1/2 were also observed. The small fusion protein designed to cover the presumed AMDV VP1/2 loop 4 was purified by affinity chromatography and used to develop solid-phase immunoassays. Twelve small synthetic peptides were constructed and used as inhibitors. A peptide covering amino acids S428 to T448 was shown to block the reactivity of a pool of positive mink sera, indicating the presence of one dominant linear epitope.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epitope Mapping of Aleutian Mink Disease Parvovirus Virion Protein VP1 and 2

Costello¹,

Steenfos²,

Jensen³

et al. 1999

Scand J Immunol

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“…The complete sequence and transcription map of the 4,748-nucleotide (nt) nonpathogenic ADV-G isolate of ADV are known (4,16,18). Furthermore, the capsid coding sequences have also been determined for the pathogenic ADV-Utah and Danish ADV-K (18,37). Isolates of ADV can be distinguished by examination of a hypervariable DNA and amino acid sequence found in a ca.…”

mentioning

confidence: 99%

“…Isolates of ADV can be distinguished by examination of a hypervariable DNA and amino acid sequence found in a ca. 600-bp EcoRV-BstEII fragment within the capsid protein gene (map units 64 to 65) (20,37,38). Definition of the hypervariable region has led to a preliminary typing scheme based on this sequence (18,19,38).…”

mentioning

confidence: 99%

“…Definition of the hypervariable region has led to a preliminary typing scheme based on this sequence (18,19,38). For example, the nonpathogenic cell culture-adapted strain, ADV-G, is defined as type 1, whereas the highly pathogenic ADV-Utah strain is designated type 2 (38), and the various sequence types appear to be quite stable (37). Interestingly, ADV-Utah and the pathogenic ADV-K were found to be mixtures of more than one sequence type on the basis of examination of the hypervariable region sequence (38).…”

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confidence: 99%

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The relationship between capsid protein (VP2) sequence and pathogenicity of Aleutian mink disease parvovirus (ADV): a possible role for raccoons in the transmission of ADV infections

Oie

Durrant

Wolfinbarger

et al. 1996

J Virol

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110

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Aleutian mink disease parvovirus (ADV) DNA was identified by PCR in samples from mink and raccoons on commercial ranches during an outbreak of Aleutian disease (AD). Comparison of DNA sequences of the hypervariable portion of VP2, the major capsid protein of ADV, indicated that both mink and raccoons were infected by a new isolate of ADV, designated ADV-TR. Because the capsid proteins of other parvoviruses play a prominent role in the determination of viral pathogenicity and host range, we decided to examine the relationship between the capsid protein sequences and pathogenicity of ADV. Comparison of the ADV-TR hypervariable region sequence with sequences of other isolates of ADV revealed that ADV-TR was 94 to 100% related to the nonpathogenic type 1 ADV-G at both the DNA and amino acid levels but less than 90% related to other pathogenic ADVs like the type 2 ADV-Utah, the type 3 ADV-ZK8, or ADV-Pullman. This finding indicated that a virus with a type 1 hypervariable region could be pathogenic. To perform a more comprehensive analysis, the complete VP2 sequence of ADV-TR was obtained and compared with that of the 647amino-acid VP2 of ADV-G and the corresponding VP2 sequences of the pathogenic ADV-Utah, ADV-Pullman, and ADV-ZK8. Although the hypervariable region amino acid sequence of ADV-TR was identical to that of ADV-G, there were 12 amino acid differences between ADV-G and ADV-TR. Each of these differences was at a position where other pathogenic isolates also differed from ADV-G. Thus, although ADV-TR had the hypervariable sequence of the nonpathogenic type 1 ADV-G, the remainder of the VP2 sequence resembled sequences of other pathogenic ADVs. Under experimental conditions, ADV-TR and ADV-Utah were highly pathogenic and induced typical AD in trios of both Aleutian and non-Aleutian mink, whereas ADV-Pullman was pathogenic only for Aleutian mink and ADV-G was noninfectious. Trios of raccoons experimentally inoculated with ADV-TR and ADV-Utah all became infected with ADV, but only a single ADV-Pullman-inoculated raccoon showed evidence of infection. Furthermore, none of the ADV isolates induced pathological findings of AD in raccoons. Finally, when a preparation of ADV-TR prepared from infected raccoon lymph nodes was inoculated into mink and raccoons, typical AD was induced in Aleutian and non-Aleutian mink, but raccoons failed to show serological or pathological evidence of infection. These results indicated that raccoons can become infected with ADV and may have a role in the transmission of virus to mink but that raccoon-to-raccoon transmission of ADV is unlikely.

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Parvoviruses

Tattersall¹,

Cotmore²

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Nucleotide sequence analysis of Aleutian mink disease parvovirus shows that multiple virus types are present in infected mink

Cited by 48 publications

References 45 publications

Epitope Mapping of Aleutian Mink Disease Parvovirus Virion Protein VP1 and 2

Epitope Mapping of Aleutian Mink Disease Parvovirus Virion Protein VP1 and 2

The relationship between capsid protein (VP2) sequence and pathogenicity of Aleutian mink disease parvovirus (ADV): a possible role for raccoons in the transmission of ADV infections

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