Nucleotide sequence and expression of two β-tubulin genes in Stylonychia lemnae

Conzelmann, Karl‐Klaus; Helftenbein, Elke

doi:10.1016/0022-2836(87)90207-5

Cited by 54 publications

(28 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macronuclear DNA was size-fractionated by preparative aga rose gel electrophoresis and cloned by G-C-tailing (a 1-tubulin gene) [18] or after Bal 31 digestion of the protruding 3'-ends as previously described [10]. Screening of recombinant plasmids was done by colony hybridization [16] with radioactive labelled cDNA probes of the a-and ß-tubulin genes [10, 18, 19] and of enriched radioactive labelled macronuclear DNA molecules containing the pob and p4A3 genes.…”

Section: Cloning and Screening Of Macronuclear Dna Moleculesmentioning

confidence: 99%

“…For denaturing gels RNA and DNA was glyoxylated [34] and gel electrophoresis and hybridization was done as previously described [10].…”

Section: Denaturing Agarose Gel Electrophoresis and Hybridization Reamentioning

confidence: 99%

“…Each DNA molecule is thought to represent a transcription and replication unit and consists of a single intronless coding region flanked by short (74 bp to 1000 bp) AT-rich (75%-80%) noncoding sequences terminating in telomeric inverted repeats of C4A4. The coding regions show, like those of some other ciliates, an altered genetic code using the "universal" stop codons TAA and TAG to specify glutamine [8,10,18,19,22,25,36,40].…”

Section: Introductionmentioning

confidence: 97%

“…In the hypotrichous ciliate Stylonychia lemnae this macronuclear development includes chromosome polytenization, degradation of the giant chromosomes, selective DNA elimination, DNA rearrangement and DNA amplification [41]. The resulting transcriptionally active macronuclear genome is organized in about 15 000 different DNA molecules of 0.4 to 20 kbp in length, of which each is present in a distinct copy number of a few to more than 100 000 [3,10,18,19,30,48]. Each DNA molecule is thought to represent a transcription and replication unit and consists of a single intronless coding region flanked by short (74 bp to 1000 bp) AT-rich (75%-80%) noncoding sequences terminating in telomeric inverted repeats of C4A4.…”

Section: Introductionmentioning

confidence: 99%

“…Four of the investigated Stylonychia genes represent the two a-and the two ß-tubulin genes, each of them present in a high (ar and βι 150 000) and in a low copy number (a2-and ß2 30 000) [10,18,19]. With respect to the biological function of the a-and ß-tubulins the coordinated expression of their genes is expected.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Helftenbein

Conzelmann

Becker

et al. 1989

European Journal of Protistology

Self Cite

View full text Add to dashboard Cite

Section: Cloning and Screening Of Macronuclear Dna Moleculesmentioning

confidence: 99%

“…For denaturing gels RNA and DNA was glyoxylated [34] and gel electrophoresis and hybridization was done as previously described [10].…”

Section: Denaturing Agarose Gel Electrophoresis and Hybridization Reamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Helftenbein

Conzelmann

Becker

et al. 1989

European Journal of Protistology

Self Cite

View full text Add to dashboard Cite

Identification of an amino acid substitution in the benA, β‐tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity

Jung

Oakley

1990

Cell Motil. Cytoskeleton

101

View full text Add to dashboard Cite

We are using molecular genetic techniques to identify sites of interaction of beta-tubulin with benzimidizole anti-microtubule agents. We have developed a marker-rescue technique for cloning mutant alleles of the benA, beta-tubulin gene of Aspergillus nidulans and have used the technique to clone two mutant benA alleles, benA16 and benA19. These are the only A. nidulans alleles known to confer resistance to the benzimidazole antimicrotubule agent thiabendazole and supersensitivity to other benzimidazole antimicrotubule agents including benomyl and its active breakdown product, carbendazim. benA16 has been shown, moreover, to reduce thiabendazole binding to beta-tubulin. We have sequenced the two mutant alleles and have found that they carry different nucleotide changes that cause the same single amino acid substitution, valine for alanine at amino acid 165. Since thiabendazole and carbendazim differ at only one side chain, the R2 group, we conclude that the region around amino acid 165 is involved in the binding of the R2 group of benzimidazole antimicrotubule agents to beta-tubulin.

show abstract

Perspectives on tubulin isotype function and evolution based on the observation that Tetrahymena thermophila microtubules contain a single α‐ and β‐tubulin

Gaertig

Thatcher

McGrath

et al. 1993

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

We have cloned and sequenced the two beta-tubulin genes of the ciliated protozoan Tetrahymena thermophila. The two genes encode identical 443 amino acid peptides which are 99.7% identical to the beta-tubulin proteins of T. pyriformis and 95% identical to human beta 1 tubulin. T. thermophila contains only one alpha-tubulin gene (Callahan et al., 1984: Cell 36:441-445). Thus, all of the extremely diverse microtubule structures in this unicellular organism can be formed from a single alpha- and a single beta-tubulin peptide. We have also carried out a phylogenetic analysis of 84 complete beta-tubulin peptide sequences. This analysis supports two hypotheses regarding beta-tubulin evolution and function: 1) Multifunctional beta-tubulins are under greater evolutionary constraint than beta-tubulins present in specialized cells or in cells with very few microtubule related functions, which can evolve rapidly; and 2) Cells which form axonemes maintain a homogeneous population of tubulins.

show abstract

Nucleotide sequence and expression of two β-tubulin genes in Stylonychia lemnae

Cited by 54 publications

References 44 publications

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Identification of an amino acid substitution in the benA, β‐tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity

Perspectives on tubulin isotype function and evolution based on the observation that Tetrahymena thermophila microtubules contain a single α‐ and β‐tubulin

Contact Info

Product

Resources

About