Nucleotide sequence and genome structure of grapevine rupestris stem pitting associated virus-1 reveal similarities to apple stem pitting virus.

Meng, Bin; Pang, Sheng‐Zhi; Forsline, Philip L.; McFerson, James R.

doi:10.1099/0022-1317-79-8-2059

Cited by 80 publications

(73 citation statements)

References 21 publications

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“…It was reported that the genomic diversity of GVA has been examined based on the analysis of partial or complete coat protein gene (CP), because it had a certain degree of variability (Goszczynski and Jooste, 2003;Murolo et al, 2008). It was reported that the genomic diversity of GRSPaV has been examined based on the analysis of partial or complete RNA-dependent RNA polymerase gene (RdRp) (ORF 1), which is one of the most conserved regions of the GRSPaV genome (Meng et al, 1998;Lunden et al, 2010). Characterizing virus isolates is an important aspect of the development of tools for the detection of this virus, the information about sequence diversity among variants may help to select regions of the virus genome targeted for specific virus detection and to design primers for reliable detection of all known virus variants.…”

Section: Discussionmentioning

confidence: 99%

Detection and molecular characterization of Egyptian isolates of grapevine viruses

Fattouh¹,

Ratti²,

ElAhwany³

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Detection and molecular characterization of Egyptian isolates of grapevine viruses

Fattouh¹,

Ratti²,

ElAhwany³

et al. 2014

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“…O Rupestris stem pitting-associated virus (RSPaV), família Flexiviridae, gênero Foveavirus, agente causal das caneluras do tronco de Rupestris, possui partículas alongadas e flexuosas com cerca de 800 x 12 nm, CP de 28 kDa e genoma de RNA de fita simples e senso positivo com 8726 nucleotídeos (nt), organizado em cinco ORFs. A ORF5 (nt 7770 a 8549) A ORF5 (nt 7770 a 8549) codifica o gene CP (Meng et al, 1998;Zhang et al, 1998;Fauquet et al, 2005).…”

Section: Introductionunclassified

Variabilidade do gene da proteína capsidial de três espécies virais que infectam videiras no Brasil

Radaelli¹,

Fajardo²,

Nickel³

et al. 2009

Trop. plant pathol.

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RESUMOO objetivo deste trabalho foi avaliar a variabilidade de três vírus (Rupestris stem pitting-associated virus -RSPaV, Grapevine leafroll-associated virus 2 -GLRaV-2 e Grapevine fanleaf virus -GFLV) que infectam videiras no Brasil, através da caracterização molecular do gene da proteína capsidial (CP). Foram amplificados fragmentos que compreendem os genes completos da CP de nove isolados de RSPaV (780 pb), seis de GLRaV-2 (597 pb) e três de GFLV (1515 pb) por RT-PCR, utilizando-se oligonucleotídeos específicos para cada espécie viral. Os DNAs amplificados foram clonados e sequenciados. Os isolados de RSPaV foram reunidos em quatro grupos pela análise filogenética das sequências obtidas com valores de identidade de nucleotídeos (nt) que variaram de 81 a 99%. Para GLRaV-2, dois grupos foram definidos a partir das sequências de nt, com valores de identidade que variaram de 88 a 99% e, para GFLV, foram definidos dois grupos, com valores de identidade de nt que variaram de 89 a 98%. Os diferentes isolados das três espécies virais estudadas também foram detectados utilizando-se hibridização com sondas não-radioativas, marcadas com digoxigenina, possibilitando identificar de forma inequívoca as amostras infectadas, independentemente dos isolados virais empregados para síntese das sondas. Palavras-chave: RSPaV, GLRaV-2, GFLV, RT-PCR, Vitis spp., sonda não-radioativa. ABSTRACT Coat protein gene variability of three viral species infecting grapevines in BrazilThe purpose of this study was to evaluate the variability of three viruses (Rupestris stem pitting-associated virus -RSPaV, Grapevine leafroll-associated virus 2 -GLRaV-2 and Grapevine fanleaf virus -GFLV) infecting grapevines in Brazil, through molecular characterization of the coat protein (CP) gene. DNA fragments were amplified comprising the complete CP genes of nine isolates of RSPaV (780 bp), six of GLRaV-2 (597 bp) and three of GFLV (1515 bp) by RT-PCR, using specific primers for each viral species. The amplified DNA fragments were cloned and sequenced. RSPaV isolates were clustered into four groups by phylogenetic analysis of the nucleotide (nt) sequences of the CP gene, showing identity values ranging from 81 to 99%. For GLRaV-2, two groups were defined from nt sequences, with identity values ranging from 88 to 99% and for GFLV, two groups were defined with identity values ranging from 89 to 98%. The isolates of each viral species studied here were detected by non-radioactive probes labeled with digoxigenin, allowing unambiguous identification of infected samples, independent of the isolate used as template for probe synthesis.

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“…These included development of rapid serological detection of viruses infecting a range of plants, including woody plants (62), vegetables (51), and others such as cardamom (28); development of pseudorecombinants of multicomponent viruses (6, 32); and cross-protection studies on zucchini yellow mosaic (55), tomato ringspot (2), and tomato spotted wilt (57) viruses. Later work led to development and testing of virus-resistant transgenic vegetables (12-14, 22, 58); some work on the mechanism of transgenic virus resistance (40)(41)(42); sequencing of viral genomes (33,38,63); and risk assessment studies of virus-resistant transgenic plants (11). I also did a lot of international work on a range of viruses, which helped to attract numerous graduate students, postdoctoral associates, and scientists to my laboratory.…”

Section: Synopsis Of My Research Effortsmentioning

confidence: 99%

The Wayward Hawaiian Boy Returns Home

Gonsalves

2015

Annu. Rev. Phytopathol.

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This chapter represents a travelog of my life and career and the philosophical points I acquired along the way. I was born on a sugar plantation on the island of Hawaii and early on had a stuttering problem. I attended the Kamehameha Schools and received my BS and MS degrees from the University of Hawaii and my Ph.D. from the University of California at Davis. I link my life and career to various principles and events, some of which are: the importance of positioning oneself; going for the big enchilada; music, the international language; the red zone of biotechnology; the human side of biotechnology; the transgenic papaya story; and my leadership time at USDA in Hawaii. The guiding light throughout my career were the words from Drs. Eduardo Trujillo and Robert Shepherd, respectively, "Dennis, don't just be a test tube scientist, do something to help people" and "

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Nucleotide sequence and genome structure of grapevine rupestris stem pitting associated virus-1 reveal similarities to apple stem pitting virus.

Cited by 80 publications

References 21 publications

Detection and molecular characterization of Egyptian isolates of grapevine viruses

Detection and molecular characterization of Egyptian isolates of grapevine viruses

Variabilidade do gene da proteína capsidial de três espécies virais que infectam videiras no Brasil

The Wayward Hawaiian Boy Returns Home

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