Nucleotide Sequence and Variation of IGF2 Gene Exon 6 in Bos Taurus and Bos Indicus Cattle

Abstract

The major assumption of this study is that polymorphism of a gene could be used to investigate its allele-specific expression as well as its methylation and imprinting status. Therefore, the aim of this study was to analyze the polymorphism of the coding region of the bovine IGF2 gene and to determine the sequence of its gene exon 6 in Bos taurus and Bos indicus cattle. A single nucleotide "C" deletion/insertion polymorphism was found in both cattle subspecies and a G/T transversion (RFLP-MboII) in the Bos indicus IGF2 gene. A 407-bp fragment of bovine IGF2 exon 6 was sequenced and the sequences (including variable nucleotides) were deposited in the GenBank database. A comparative analysis was performed for this fragment from different species; 99.5% identity was found between Bos taurus and Bos indicus cattle.

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

“…The results of the Bonferroni test were found to be significant (5%), and each animal that received the C allele tended to weigh on average 10.2 kg less than its contemporary. Several studies have investigated allelic profiles by SNP genotyping in cattle populations (Damiani et al, 2000;Flisikowski et al, 2005;de Souza et al, 2007;Andréa et al, 2007;Biase et al, 2007;Curi et al, 2009;Bagnicka et al, 2010;Souza et al, 2010). Up to 2009, around 500 bulls from 123 farms participating in the Genetic Improvement Program of the Nellore Breed in Brazil have been evaluated for the future multiplication of "positive animals" in commercial herds by artificial insemination, and of this total number, 25% were evaluated in the present study.…”

“…The restriction fragment length polymorphism (RFLP)/MboII polymorphism is located in exon 6 of the IGF2 gene (insulin-like growth factor 2), located on bovine chromosome 29, which plays an important and essential role in cell proliferation and differentiation for growth and embryonic development in mammals (Flisikowski et al, 2005). The RFLP/HpaII polymorphism is in the Bov-A2 element (considered to be a short interspersed nucleotide element, SINE) present in the promoter region of the CYP21 gene (steroid 21-hydroxylase) on bovine chromosome 23.…”

Frequencies of candidate genes and associations with carcass and meat traits in Nellore and crossbred cattle

“…To identify the G and T alleles of the IGF2/MboII polymorphism of IGF2, a 407-base pair (bp) fragment, corresponding to a region located on exon 6, was amplified and digested with the MboII restriction enzyme, by the method outlined in Flisikowski et al (2005). The A and T alleles of the DQ499531.1:g.134A > T SNP, located in the (5') regulatory region of PMCH were identified by the amplification of a 424-bp fragment and digested with the TaiI enzyme, as in Helgeson & Schmutz (2008).…”

“…The protein encoded by IGF2 is a fetal-growth and differentiation factor that has an important role in muscle growth, as well as in the proliferation and differentiation of myoblasts (Flisikowski et al, 2005). In a study performed by Nezer et al (1999), a QTL with stronger effects on muscle mass and fat deposition in the carcass was mapped to the region of the IGF2 locus in swine.…”

“…According to these studies, insulin-like growth factor 2 (IGF2), pro-melanin-concentrating hormone (PMCH), and RAR-related orphan receptor C (RORC) are candidate genes that are possibly associated with traits of interest in livestock (Flisikowski et al, 2005;Barendse et al, 2006;Helgeson & Schmutz, 2008).…”