Numerical Classification of Sporoactinomycetes Containing meso-Diaminopimelic Acid in the Cell Wall

Goodfellow, Michael; Pirouz, T.

doi:10.1099/00221287-128-3-503

Cited by 52 publications

(30 citation statements)

References 62 publications

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“…The present study provides further evidence that the genus Saccharomonospora encompasses undescribed species (15,27,36). The separation of Saccharomonospora sp.…”

Section: Resultsmentioning

confidence: 62%

A Phylogenetic Analysis of the Genus Saccharomonospora Conducted with 16S rRNA Gene Sequences

Kim

Yoon

Kim³

et al. 1995

International Journal of Systematic Bacteriology

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Nearly complete sequences of 16s rRNA genes of representative strains of the genus Saccharomonospora were determined following the isolation and cloning of the amplified genes. The sequences were aligned with those of representatives of the family Pseudonocardiaceae, and a phylogenetic tree was inferred by the neighborjoining method. The genus Saccharomonospora formed a distinct clade within the evolutionary radiation encompassed by the family Pseudonocardiaceae. The average nucleotide similarity value found between the type strains of the four validly described Saccharomonospora species was 97.5% k 1.0%. The most distant relationship was found between Saccharomonospora azurea and Saccharomonospora viridis K73 (96.3% similarity). In contrast, Sacchuromonospora azurea K161 and "Saccharomonospora caesia" K163 had identical 16s rRNA gene sequences. The nucleotide sequence data suggest that the genus Saccharomonospora contains several new centers of variation.The genus Saccharomonospora was proposed by Nonomura and Ohara (30) for monosporic actinomycetes that contained meso-diaminopimelic acid, arabinose, and galactose in the peptidoglycan (wall chemotype IV sensu Lechevalier Lechevalier [25]). Subsequently, additional chemical markers were found to characterize the taxon, notably the presence of major amounts of iso-and anteiso-fatty acids, 2-hydroxy fatty acids, phosphatidylethanolamine, and menaquinones that were tetrahydrogenated with nine isoprene units (21,25).There are currently four validly described Saccharomonospora species, namely, Saccharomonospora azurea (32), Saccharomonospora cyanea (33), Saccharomonospora glauca (17), and Saccharomonospora viridis (30), the type species of the genus. A fifth species, "Saccharomonospora caesia," was proposed by for organisms previously classified as Micropolyspora caesia (20,22); this organism did not appear in the Approved Lists of Bacterial Names (35) and has not been added subsequently, and hence, it has not been validly published. It is important to distinguish between members of validly described and putatively new Saccharomonospora species, as some kinds of saccharomonosporae can cause hypersensitivity pneumonitis. S. viridis is strongly implicated as one the causal agents of farmer's lung disease (3,16).It is also important to determine the taxonomic structure of the genus Saccharomonospora in order to discover the relationships between the existing taxa and between them and prospective new species. It appears from preliminary 16s rRNA sequence studies that the genus Saccharomonospora forms a distinct phyletic line within the evolutionary radiation encompassed by the family Pseudonocardiaceae (5, 9, 10, 28, 36). To date, three Saccharomonospora strains have been the subject of 16s rRNA sequencing analyses, the type strain of S. viridis, the putative type strain of "S. caesia," and an isolate from stored grain, Saccharomonospora sp. strain A1206, that was tentatively considered, subject to additional sequencing studies, to be the nucleus of a new species (36). ...

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“…The present study provides further evidence that the genus Saccharomonospora encompasses undescribed species (15,27,36). The separation of Saccharomonospora sp.…”

Section: Resultsmentioning

confidence: 62%

A Phylogenetic Analysis of the Genus Saccharomonospora Conducted with 16S rRNA Gene Sequences

Kim

Yoon

Kim³

et al. 1995

International Journal of Systematic Bacteriology

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“…nov., with strain JCM 3148 (= ATCC 27300) as the type strain. Goodfellow & Pirouz (1982) found using numerical classification that three strains of Microbispora were recovered in a well-defined cluster, whereas M . echinospora and M .…”

Section: Taxonomic Status Of M Uiridis and M Echinosporamentioning

confidence: 99%

A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea

Miyadoh

Amano²,

Tohyama³

et al. 1990

Journal of General Microbiology

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We conducted a taxonomic review of the genus Microbispora using chemotaxonomic and DNA-DNA hybridization techniques, and reached the following conclusions: Microbispora uiridis should be transferred to the genus Actinomadura as Actinomadura rugatobispora comb. nov., nom. nov. (type strain SF2240 = I F 0 14382 = JCM 3366) and Microbispora echinospora should be transferred to the genus Actinomadura as Actinomadura echinospora comb. nov. (type strain JCM 3148 = ATCC 27300). We also propose that Microbispora rosea, Microbispora amethystogenes, Microbispora chromogenes, Microbispora diastatica, Microbispora indica, Microbispora karnatakensis and Microbispora parva should be combined into the species Microbispora rosea subsp. rosea (type strain JCM 3006 = ATCC 12950), and that Microbispora amata, Microbispora thermodiastatica and Microbispora thermorosea should be combined and transferred to the new subspecies Microbispora rosea subsp. aerata comb. nov. (type strain I F 0 12581 = ATCC 15448). Microbispora bispora clearly differs from these ten strains at the species level.

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“…Decomposition of adenine, casein, hypoxanthine, tyrosine, urea and xanthine was examined by using the methods of Gordon et al (1974). Decomposition of gelatin, elastin, aesculin and starch was examined by using the methods of Goodfellow & Pirouz (1982). Enzyme activity tests were performed using API ZYM test kits (bioMérieux).…”

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confidence: 99%

Rhodococcus kunmingensis sp. nov., an actinobacterium isolated from a rhizosphere soil

Wang

Zhang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive, aerobic, non-motile actinobacterium strain, designated YIM 45607 T , was isolated from a rhizosphere soil sample in Kunming, south-west China. Chemotaxonomically, the isolate contained chemical markers that supported its assignment to the genus Rhodococcus. On the basis of 16S rRNA gene sequence similarity analysis, strain YIM 45607 T formed a new subline within the genus Rhodococcus, with Rhodococcus equi as its closest phylogenetic neighbour (98.2 % 16S rRNA gene sequence similarity to the type strain). However, DNA-DNA hybridization demonstrated that strain YIM 45607 T was different from R. equi DSM 20307 T (35.4 % relatedness). Based on polyphasic analysis, strain YIM 45607 T could be clearly distinguished from other species of the genus Rhodococcus. The isolate therefore represents a novel species of Rhodococcus, for which the name Rhodococcus kunmingensis sp. nov. is proposed. The type strain is strain YIM 45607 T (5KCTC 19149 T 5DSM 45001 T ).The genus Rhodococcus was classified into the family Nocardiaceae of the suborder Corynebacterineae (Stackebrandt et al., 1997). With the emergence of molecular identification methods, particularly 16S rRNA gene sequencing, the classification of the rhodococci has been greatly improved. For example, members of the genus Rhodococcus have been assigned to four 16S rRNA subclades, represented by Rhodococcus equi, Rhodococcus rhodnii, Rhodococcus rhodochrous and Rhodococcus erythropolis (McMinn et al., 2000), and the discovery of novel Rhodococcus species has been greatly facilitated. At the time of writing, more than 40 species are recognized and these micro-organisms exhibit broad metabolic diversity. In this study, the taxonomic position of an actinobacterium was examined by using a polyphasic approach. On the basis of phenotypic, chemotaxonomic and genotypic characteristics, it is proposed that strain YIM 45607 T represents a novel species of the genus Rhodococcus.Strain YIM 45607 T was isolated from a soil sample collected from the rhizosphere of Taxus chinensis in Kunming by the following method. Soil samples were air-dried for 7 days; 1 g soil was then suspended in 50 mM phosphate buffer (pH 7.0) containing 0.1 % sodium cholate and incubated at 45 u C for 1 h with vigorous shaking in order to disperse soil aggregates and restrain the growth of fast-growing bacteria. The soil-water suspension was centrifuged and 0.1 ml supernatant was resuspended in 9 ml of the same sterile buffer before being spread on humic acid-vitamins-gellan gum (HVG) medium (Suzuki et al., 1999) and incubated at 28 u C for 30 days. The strain was cultivated on yeast extract-malt extract agar (ISP 2;Shirling & Gottlieb, 1966) and maintained as a glycerol suspension (20 %, w/v) at 270 u C.To investigate its morphological properties, strain YIM 45607 T was cultivated aerobically at 28 u C on ISP 2. Cell morphology was examined by using light microscopy with a model BH-2 microscope (Olympus) and scanning electron microscopy (Philips XL30; ESEM-TMP). Growth on ISP 2, ISP 5 (Shirli...

show abstract

Numerical Classification of Sporoactinomycetes Containing meso-Diaminopimelic Acid in the Cell Wall

Cited by 52 publications

References 62 publications

A Phylogenetic Analysis of the Genus Saccharomonospora Conducted with 16S rRNA Gene Sequences

A Phylogenetic Analysis of the Genus Saccharomonospora Conducted with 16S rRNA Gene Sequences

A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea

Rhodococcus kunmingensis sp. nov., an actinobacterium isolated from a rhizosphere soil

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