Numerical simulation of the effect of superparamagnetic nanoparticles on microwave rewarming of cryopreserved tissues

Wang, Tao; Zhao, Gang; Liang, Xin; Xu, Yunpeng; Yang, Li; Tang, Heyu; Jiang, Rui; Gao, Dayong

doi:10.1016/j.cryobiol.2014.02.002

Cited by 33 publications

(11 citation statements)

References 56 publications

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“…Heating magnetic nanoparticles can be conveniently realized with an induction apparatus over a medium frequency range (several hundreds of kHz), and has been investigated to treat tumors [42, 43, 46–50]. Magnetic nanoparticles have also been shown to improve the efficiency of the microwave rewarming process, and augment tumor treatment with cryosurgery [51, 52]. However, the effect of magnetic induction heating (MIH) of SPM nanoparticles in an AC magnetic field on vitrified cryopreservation has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Wang

Zhao

Zhang³

et al. 2016

Acta Biomaterialia

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Cryopreservation by vitrification has been recognized as a promising strategy for long-term banking of living cells. However, the difficulty to generate a fast enough heating rate to minimize devitrification and recrystallization-induced intracellular ice formation during rewarming is one of the major obstacles to successful vitrification. We propose to overcome this hurdle by utilizing magnetic induction heating (MIH) of magnetic nanoparticles to enhance rewarming. In this study, superparamagnetic (SPM) Fe3O4 nanoparticles were synthesized by a chemical coprecipitation method. We successfully applied the MIH of Fe3O4 nanoparticles for rewarming human umbilical cord matrix mesenchymal stem cells (hUCM-MSCs) cryopreserved by vitrification. Our results show that extracellular Fe3O4 nanoparticles with MIH may greatly suppress devitrification and/or recrystallization during rewarming and significantly improve the survival of vitrified cells. We further optimized the concentration of Fe3O4 nanoparticles and the current of an alternating current (AC) magnetic field for generating the MIH to maximize cell viability. Our results indicate that MIH in an AC magnetic field with 0.05% (w/v) Fe3O4 nanoparticles significantly facilitates rewarming and improves the cryopreservation outcome of hUCM-MSCs by vitrification. The application of MIH of SPM nanoparticles to achieve rapid and spatially homogeneous heating is a promising strategy for enhanced cryopreservation of stem cells by vitrification.

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Section: Introductionmentioning

confidence: 99%

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Wang

Zhao

Zhang³

et al. 2016

Acta Biomaterialia

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“…(15), if the heat generated by the NPs is greater than the heat generated by the sample, i.e., Q nano ! Q wave [38]:…”

Section: Heat Generated By Spm Nps In the Microwave Fieldmentioning

confidence: 99%

“…Hence, in most cases, it is likely that these nanometer-sized particles will evenly distribute in 1.5 mL sized small biomaterial in a reasonable short period of time. Furthermore, the density (r 3 ), the average specific heat (C p3 ), and the thermal conductivity (k 3 ) of the frozen sample embedded with NPs can be approximated by the combination of the sample and the NPs [37,38,42,43]:…”

Section: Heat Generated By Spm Nps In the Microwave Fieldmentioning

confidence: 99%

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Theoretical investigation of a novel microwave antenna aided cryovial for rapid and uniform rewarming of frozen cryoprotective agent solutions

Wang

Zhao

Deng

et al. 2015

Applied Thermal Engineering

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“…Volumetric heating can potentially increase the overall rewarming rate, while moderating temperature gradients within the specimen. Previous experimental studies have explored the feasibility of volumetric heating by means of electromagnetic radiation in the radio frequency (RF) range [13], [35] while some studies focused on the microwave range [6], [7], [18], [22], [36], [40], benefiting from its readily accessible commercial technology. While microwave and wider RF-range applications may benefit cryopreservation applications, they are also associated with so-called hot spots , due to inhomogeneity in the electrometric-field intensity [13], [34].…”

Section: Introductionmentioning

confidence: 99%

Thermo-mechanical stress analysis of cryopreservation in cryobags and the potential benefit of nanowarming

2017

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Cryopreservation by vitrification is the only promising solution for long-term organ preservation which can save millions of lives across the world. One of the challenges in cryopreservation of large-size tissues and organs is to prevent fracture formation due to the tendency of the material to contract with temperature. The current study focuses on a pillow-like shape of a cryobag, while exploring various strategies to reduce thermo-mechanical stress during the rewarming phase of the cryopreservation protocol, where maximum stresses are typically found. It is demonstrated in this study that while the level of stress may generally increase with the increasing amount of CPA filled in the cryobag, the ratio between width and length of the cryobag play a significant role. Counterintuitively, the overall maximum stress is not found when the bag is filled to its maximum capacity (when the filled cryobag resembles a sphere). Parametric investigation suggests that reducing the initial rewarming rate between the storage temperature and the glass transition temperature may dramatically decrease the thermo-mechanical stress. Adding a temperature hold during rewarming at the glass transition temperature may reduce the thermo-mechanical stress in some cases, but may have an adverse effect in other cases. Finally, it is demonstrated that careful incorporation of volumetric heating by means on nanoparticles in an alternating magnetic field, or nanowarming, can dramatically reduce the resulting thermo-mechanical stress. These observations display the potential benefit of a thermo-mechanical design of the cryopreservation protocols in order to prevent structural damage.

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Numerical simulation of the effect of superparamagnetic nanoparticles on microwave rewarming of cryopreserved tissues

Cited by 33 publications

References 56 publications

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Theoretical investigation of a novel microwave antenna aided cryovial for rapid and uniform rewarming of frozen cryoprotective agent solutions

Thermo-mechanical stress analysis of cryopreservation in cryobags and the potential benefit of nanowarming

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