Numerical Taxonomy of Moderately Halophilic Gram-negative Rods

Abstract

A study was made of 516 randomly selected isolates of moderately halophilic bacteria from solar salterns showing salinities between 8.8 and 40.0% (w/v) total salts, located in S.E. Spain. After purification, many cytological, physiological, biochemical, nutritional and an ti biot ic sensitivity characters were determined for 106 selected saltern isolates and two reference strains. Data were coded and analysed by numerical techniques using the Jaccard coefficient ( S J ) , and clusters of strains were obtained by average linkage (UPGMA) analysis. Nine major phenons were found at the 72.5 % similarity level. The properties of each phenon are given, their taxonomic affinities are discussed, and typical reference strains are suggested. Almost all the strains were related to genera known to contain marine species. A large number of the strains could be tentatively assigned to the genus Vibrio, suggesting that this may be an abundant taxon of moderately halophilic Gram-negative rods in solar salterns.

Comparison of Bacterial Biodiversity and Enzyme Production in Three Hypersaline Lakes; Urmia, Howz-Soltan and Aran-Bidgol

“…Gram reaction, motility, colony morphology and pigmentation, catalase, urease and oxidase activities, nitrate reduction, Tween 80 hydrolysis, Voges-Proskauer and methyl red reaction were checked as recommended by Sanchez-Porro et al [23]. Acid production from carbohydrates, carbon and nitrogen sources utilization were performed according to Ventosa et al [24]. Also, the susceptibility of the strains to antibiotics was determined on Muller-Hinton agar containing 10 and 20 % NaCl.…”

Isolation of Moderately Halophilic Pseudoalteromonas Producing Extracellular Hydrolytic Enzymes from Persian Gulf

“…Proteolytic activity of the cultures was screened qualitatively in a saline medium containing skim milk (10%) plus 10% (w/v) total salts [11]. Formation of clear zone around the colonies over the next 48 h was considered as indication of protease production ( Fig.…”

Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate

“…Unless otherwise stated, all morphological examinations, biochemical and tolerance tests were performed as described previously (Santos et al 1989;Nunes et al 1992), in 10% MH medium (Ventosa et al 1982) (http:// www.dsmz.de/media/med593.htm) as follows (%, w/v): MgCl 2 , 0.7; MgSO 4 , 0.96; CaCl 2 , 0.036; KCl, 0.2; NaHCO 3 , 0.006; NaBr, 0.0026; proteose-peptone no. 3, 0.5; yeast extract, 1.0 and glucose, 0.1.…”

“…Results were recorded after 4 h incubation at 37°C. Single carbon source assimilation tests were performed in a basal liquid medium described by Ventosa et al (1982) to which filter-sterilized yeast extract (0.1 g/l), ammonium chloride (0.5 g/l) and the carbon sources (2.0 g/l) were added. Growth of the strains was examined by measuring the turbidity of cultures incubated at 37°C as described previously (Moreira et al 2000).…”

“…Degryse 162 medium basal salts and ABM2 basal medium (Tiago et al 2004) were also used to assess the assimilation of single carbon sources. API 50 CH test strips (Analytab Products Inc., BioMe´rieux) were also used to examine the assimilation of carbohydrates and to examine the production of acid using a defined medium (Ventosa et al 1982) containing the following components per litre: NaCl, 40.0 g (for strains RH1 T and RH4) or 80.0 g (for S. roseus and S. hispanicus); MgSO 4 AE7H 2 O, 2.0 g; CaCl 2 AE2H 2 O, 0.5 g; FeCl 3 AE6H 2 O, traces; KH 2 PO 4 , 0.5 g; proteose-peptone no. 3, 1.0 g; yeast extract, 0.1 g; agar, 3.0 g; phenol red, 0.01 g.…”