Nutritional compositions of Indian Moringa oleifera seed and antioxidant activity of its polypeptides

Abstract

To study the nutritional composition of Indian Moringa oleifera seed and the antioxidant activity of M. oleifera seed polypeptide, Indian M. oleifera seed was used as raw material for composition analysis and content determination. After extraction of the seed protein, enzymatic hydrolysis with flavourzyme, dispase, papain, pepsin, and alcalase was conducted for different time, and the optimal enzymatic hydrolysis conditions was determined with DPPH scavenging capacity as an indicator. The seed polypeptides obtained by enzymatic hydrolysis were ultrafiltered, and the active peptide fragments were tracked with DPPH, HO (•OH), ABTS and superoxide anion (O 2 • − ) free radical scavenging ability and lipid oxidation inhibition rate as indicators. The results showed that the protein content in Indian M. oleifera seed was high to 40.34%, containing seven essential amino acids. The content of macroelements such as potassium, sodium, and magnesium is high, with the potassium content as high as 2,357.71 mg/kg, among the microelements, the iron content as high as 36.2 mg/kg. The optimum enzymatic hydrolysis conditions were as follows: enzymatic hydrolysis with flavourzyme (50°C, pH 6.7) for 300 min, and DPPH scavenging capacity was 84.76%. Activity tracing found that the polypeptide fragment with molecular weight <3.5 kDa had the strongest antioxidant capacity, and the EC 50 values of DPPH, •OH, ABTS, and O 2 • − free radical scavenging rates were 4.0, 4.2, 5.3, and 4.3 mg/ml, respectively. The above results show that Indian M. oleifera seed not only has high nutritional value, but its protease enzymatic hydrolyzate also has significant antioxidant activity, which can be further developed into nutrition products, healthcare products, functional foods, beauty and skin care products, liver protection drugs, etc.

Potential of Germination in Selected Conditions to Improve the Nutritional and Bioactive Properties of Moringa (Moringa oleifera L.)

“…( Table 1) was similar to values reported earlier [8,26,28]. In contrast, Olagbemide and Alikwe [24] and Liang et al [23] reported lower fat levels in moringa seeds than the ones of the present study. Fat content in moringa seed varied depending on germination conditions.…”

Improvement in overall performance of Catla catla fingerlings fed phytase included low cost plant by products-based diet

“…M. oleifera seed meal (MOSM) is an excellent protein source constituting almost 33 to 38%, amino acids i.e. tryptophan, cysteine, methionine, and considerable vitamins ( Hassan et al, 2018 , Liang et al, 2019 ), various phenolic compounds and beta carotene ( Anwar et al, 2007 , Compaore et al, 2011 ). Composition of Moringa seed-cake has shown high amount of amino acids, as described in leaves, that are relatively lower in other plant-based diets ( Ferreira et al, 2008 ).…”

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

“…Certain important amino acids e.g., methionine, cysteine and glycine and some essential minerals are also present in seeds (Hassan et al 2018;Liang et al 2019). Leaves of Moringa have high quantity of protein varying from 23% (Hassan et al 2018) 32% (Soliva et al 2005) and higher content of minerals and nutrients (Liang et al 2019). Minerals that Moringa seeds carry include Na, P, Zn, Mg, K, Fe and Ca etc.…”