Nutritional diagnosis based on sap analysis for containerized <i>Cupressus glabra</i> and <i>cupressocyparís leylandü</i> with fertigation systems

Eymar, E.; Cadahía, C.; Sanchez, Africa; López‐Vela, Dolores

doi:10.1080/00103620009370509

Cited by 2 publications

(7 citation statements)

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“…Plant sap analysis is an operationally defined method, meaning that the analysis results will highly depend on the chosen methodology since it has not been standardized. There is still no consensus among the scientific community regarding a unique sap analysis methodology for sample collection, tissue type (petioles, shoot tips, and leaf blades), pressing equipment, sap extraction, or fluids analyses [19,27,28,34,47]. Therefore, our goal is to describe the different definitions and methodologies involved in sap analysis so that readers and the scientific community can have a baseline to start defining a general, standardized, and consented methodology.…”

Section: Procedures For Sap Analysismentioning

confidence: 99%

“…The sampling strategy must consider and separate potential differences typically found in groves, such as soil types, cultivars, and management practices [26]. The samples should be taken at a similar stage within the same group of well-watered trees because sap nutrient concentration may vary depending on the crop stage, some of them declining with the growth stage and time [26,27,39,49,52]. At the sample collection, we should consider the type of tissue and timing.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Later, the sample is defrosted, and the ether and chlorophyll solution (green colored fluid) is separated from the sap by a funnel. This methodology was followed by [27,30,[56][57][58]. Some authors also froze and defrosted tissues before pressing, but they did not mention the use of ether in their methods [28,53].…”

Section: Sample Extractionmentioning

confidence: 99%

“…To optimize citrus nutrition and nutrient supply, it is essential to understand the crop nutrient requirements and have real-time diagnostic tools to determine the current nutrient status inside the plant. In this scenario, leaf and soil nutrient analysis are standard tools to assess the nutrient status of citrus trees [14,[22][23][24], but the nutrients contained in the leaf tissue may reflect an accumulation during the plant's entire cycle or season, rather than indicating the real-time concentration that is available for plant development, especially with elements such as Ca and B, which are unlikely to be remobilized once they are incorporated into the plant tissue [25][26][27]. This also applies to elements such as N, which may need more sensitive methods to determine real-time changes [2,19,[27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

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Sap Analysis: A Powerful Tool for Monitoring Plant Nutrition

et al. 2021

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Horticultural crop production is moving towards an era of higher nutrient use efficiency since nutrient deficiencies can reduce plant growth, productivity, and quality, and overfertilization can cause environmental pollution. Rapid nutrient concentration diagnostic is essential to minimize the negative effects of Huanglongbing (HLB) or citrus greening in citrus by providing the required nutrients before deficiency symptoms appear, reducing the impact of the disease on crop production. Sap analysis is an additional tool for fine-tuning nutrient applications in citrus. The main objective of this paper is to review the different methodologies and results obtained with sap analysis, considering its potential application in citrus production. Results from other crops show the pros and cons of using this tool. Substantial research has been conducted on vegetables and greenhouse crops, but few studies are available on perennial species such as citrus. Inconsistency in the extraction and analysis methods and the lack of specific sufficiency ranges for citrus open the path for further studies. Along with soil and leaf analyses, sap analysis is a complementary technique that can improve nutrient use efficiency in citrus production. Moreover, sap analysis has the potential to optimize fertilizer application, minimize environmental impacts and improve sustainability.

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Section: Procedures For Sap Analysismentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

Section: Sample Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sap Analysis: A Powerful Tool for Monitoring Plant Nutrition

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“…Leaf and root samples were oven dried (708C for 24 h) to constant weight and ground to dust. Sap samples were obtained by pressure of stems grown along the experiments (Eymar et al 2000).…”

Section: Plant Analysis and Statistical Analysismentioning

confidence: 99%

Estimation of Leaf, Root, and Sap Nitrogen Status using the SPAD‐502 Chlorophyll Meter for Ornamental Shrubs

Martín

Alonso

López

et al. 2007

Communications in Soil Science and Plant Analysis

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The SPAD-502 chlorophyll meter was evaluated as a rapid tool to predict plant nitrogen (N) concentration. The SPAD-502 index utility, as a comparative tool with respect to N nutritional status, was evaluated for Laurustine (Viburnum tinus L.), tobir (Pittosporum tobira Thumb.), and strawberry tree (Arbutus unedo L.). The effect of sampling time on the SPAD-502 index measures was also evaluated. Two sand culture experiments evaluating 1, 3, 5, and 7 mmol c . L 21 of total N concentration in nutrient solution and 70/30, 50/50, 40/60, and 30/70% nitrate/ ammonium (NO 3 2 /NH 4 þ ) ratios were applied. In a media experiment, with similar conditions and fertilization, the accuracy of the SPAD-502 regression data was evaluated. Leaf, root, and sap samples were taken in the middle and at the end of each experiment. Regression equations were established among leaf N, root N, of sap N analyzed concentrations and SPAD values with regard to global, as well as specific, data within each sampling time. Regression coefficients (b) among leaf N and SPAD were highly significant (P , 0.05) for practically all sampling times. However, liner regression equations were different in all treatments among all the sampling dates. Determination coefficients (R 2 ), in some cases, show that the SPAD-502 index is not an adequate method for leaf, root, or sap N concentration fir Viburnum tinus L. and Pittosporum tobira Thumb. or for root or sap for Arbutus unedo L. in these experimental conditions. Nonetheless, the SPAD-502 index could be a useful tool for relative comparison purposes and field operational nutrient management. However, the SPAD-502 leaf greenness meter is a relatively

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Nutritional diagnosis based on sap analysis for containerized Cupressus glabra and cupressocyparís leylandü with fertigation systems

Cited by 2 publications

References 11 publications

Sap Analysis: A Powerful Tool for Monitoring Plant Nutrition

Sap Analysis: A Powerful Tool for Monitoring Plant Nutrition

Estimation of Leaf, Root, and Sap Nitrogen Status using the SPAD‐502 Chlorophyll Meter for Ornamental Shrubs

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