Abstract. This study was aimed to evaluate the effect of diet containing waste of seaweed Gracilaria sp. on local lipid profiles of duck blood including cholesterol, triglycerides, low density lipoprotein (LDL), and high density lipoprotein (HDL). The material in this study were 72 female Tegal ducks aged 22 weeks with 1,318 ± 121 g average body weight, diets containing Gracilaria sp. waste (GW) with 18% protein and 2900 kcal/kg metabolic energy and 150 g/ton feed commercial multi-enzyme (ME). Data were subject to Completely Randomized Design with 6 treatments, (T0) Basal Diets, (T0+) Basal Diets with ME, (T1) Diets with 10% GW, (T1+) Diets with 10% GW + ME, (T2+) Diets with 12.5 % GW + ME, and (T3+) Diets with 15% GW + ME. The treatment was given for 12 weeks and blood sample was taken on the last week. The serum was separated and analyzed for blood lipid profiles using CHOD-PAP. Results showed that diet with 12.5%GW plus multi-enzyme additives significantly (P<0.05) affected blood lipid profiles of local duck, tended to lower triglycerides and LDL Cholesterol, but increase blood HDL levels.Key words: blood lipids, local ducks, Gracilaria sp. waste, multi-enzyme Abstrak. Penelitian ini bertujuan untuk mengevaluasi pengaruh ransum yang mengandung limbah rumput laut Gracilaria sp. Terhadap profi lemak darah itik local antara lain kolesterol, trigliserida, low density lipoprotein (LDL), dan high density lipoprotein (HDL).Materi yang digunakan dalam penelitian ini adalah itik Tegal betina umur 22 minggu dengan bobot badan rata-rata 1.318±121g sebanyak 72ekor, ransum mengandung limbah Gracilaria sp. (LG) dengan protein 18% dan energy metabolis 2900 kcal/kg dan multienzim (ME) komersial dengan dosis penggunaan 150 g/ton pakan. Penelitian menggunakan Rancangan Acak Lengkap dengan 6 perlakuan yaitu (T0) Ransum Basal, (T0+) Ransum Basal dengan ME, (T1) Ransumdengan 10% LG (T1+) Ransumdengan 10% LG + ME, (T2+) Ransum dengan 12,5% LG + ME (T3+) Ransum dengan 15% LG + ME. Perlakuan dilakukan selama 12 minggu dan pengambilan sampel darah pada minggu terakhir. Serum dipisahkan dan dianalisis profile mak darah menggunakan metode CHOD-PAP. Hasil menunjukkan bahwa pemberian ransum mengandung LG 12,5% ditambah aditif multienzim berpengaruh nyata (P<0,05) terhadap profil lemak darah itik lokal, cenderung menurunkan Trigliserida, Kolesterol LDL sebaliknya meningkatkan kadar HDL darah.