Nylon 6 film and nanofiber carriers: Preparation and laccase immobilization performance

Fatarella, Enrico; Spinelli, Daniele; Ruzzante, Marco; Pogni, Rebecca

doi:10.1016/j.molcatb.2014.01.012

Cited by 64 publications

(48 citation statements)

References 38 publications

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“…Analyzing the PCL nanofiber membrane, the characteristic bands were found at 2949 cm −1 , referring to the asymmetric CH 2 stretch; at 2867 cm −1 , referring to the symmetrical CH 2 vibrational stretch; at 1725 cm −1 , corresponding to the C=O bond; at 1297 cm −1 , the COC–C stretch obtained in the crystalline phase; at 1240 cm −1 , the COC asymmetric stretch and at 1173 cm −1 , corresponding to the symmetric COC stretch [Figure (a)] . Analyzing the PA‐6 nanofiber membrane [Figure (b)], characteristic peaks of PAs due to peptide bonding were observed at 3300 cm −1 (ν of the NH group), 1650 cm −1 (ν of CO–amide I group), 1550 cm −1 (δ of the NH group + ν of CN–amide II), 2857 cm −1 (symmetric CH 2 vibrational stretch), and 2930 cm −1 (nonsymmetric CH 2 vibrational stretch), according to different publications . In addition, a band in the ν region of the –OH group (3450 cm −1 ) of water was due to the hydrophilicity of the nanofiber membrane.…”

Section: Resultsmentioning

confidence: 99%

Electrospun nanofibrous membranes for solid‐phase extraction of estriol from aqueous solution

Nectoux

Medeiros

Bussamara

et al. 2018

J of Applied Polymer Sci

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Nowadays, efficient, economical, and environmentally friendly materials for the removal of emerging contaminants from the aquatic environment have been sought. Electrospun nanofibrous membranes contain fibers with diameters of submicron or nanometer scale, making them very promising adsorbent materials for use in several areas. In this context, the present study aims to synthesize and apply polymeric nanofiber membranes for solid‐phase extraction of estriol from aqueous solution. Nanofiber membranes of poly(ε‐caprolactone) (PCL) and polyamide‐6 (PA‐6) were tested as adsorbent materials and characterized by different techniques. The electrospinning time was evaluated, and the highest removal obtained for the PA‐6 nanofiber was 76.5%, spun for 100 min, whereas for the PCL nanofiber, 80% time‐independent removal was obtained. The thinner nanofibers had a larger contact area, therefore higher removals, except for the PCL nanofiber, which presented exposed beads on smaller thicknesses that impaired their efficiency. Furthermore, the nanofiber membranes have been applied for the determination of 1.0 mg L−1 of E3 in superficial water sample with satisfactory results. These aspects demonstrate that the synthesized nanofibers present an efficient material for the extraction of estriol: of high simplicity, low cost, and using green chemistry. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47189.

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Section: Resultsmentioning

confidence: 99%

Electrospun nanofibrous membranes for solid‐phase extraction of estriol from aqueous solution

Nectoux

Medeiros

Bussamara

et al. 2018

J of Applied Polymer Sci

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“…The microenvironment of the immobilized enzyme and bulk solution usually has unequal partitioning of H + and OH − concentrations due to electrostatic interactions with the matrix, which often leads to the displacements in the pH activity profile [25,27]. Furthermore, pH profiles of the immobilized laccase are broader than free enzyme, which means that the immobilization method preserved enzyme activity in a wider pH range [31,32]. These results could probably be attributed to the stabilization of laccase resulting from its multipoint attachments on the surface of poly(GMAco-nBA) microspheres.…”

Section: Effect Of Glutaric Dialdehyde As Cross-linking Agentmentioning

confidence: 99%

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

Mazlan

Hanifah

2017

International Journal of Polymer Science

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Immobilization of laccase on the functionalized methacrylate-acrylate copolymer microspheres was studied. Poly(glycidyl methacrylate-co-n-butyl acrylate) microspheres consisting of epoxy groups were synthesized using facile emulsion photocuring technique. The epoxy groups in poly(GMA-co-nBA) microspheres were then converted to amino groups. Laccase immobilization is based on covalent binding via amino groups on the enzyme surface and aldehyde group on the microspheres. The FTIR spectra showed peak at 1646 cm −1 assigned to the conformation of the polymerization that referred to GMA and nBA monomers, respectively. After modification of the polymer, intensity of FTIR peaks assigned to the epoxy ring at 844 cm −1 and 904 cm −1 was decreased. The results obtained from FTIR exhibit a good agreement with the epoxy content method. The activity of laccaseimmobilized microspheres increased upon increasing the epoxy content. Furthermore, poly(GMA-co-nBA) microspheres revealed uniform size below 2 m that contributes to large surface area of the microspheres to be used as a matrix, thus increasing the enzyme capacity and enzymatic reaction. Immobilized enzyme also shifted to higher pH and temperature compared to free enzyme.

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“…Similar increasing behaviour of the

K_{M}^{'}

value with cellulose nanofibre was reported by Sathishkumar et al, while

K_{M, 0}^{'}

= 0.343 mM was also three times higher than the value of free laccase. Much higher values were obtained for nylon nanofibre (1.07 mM) and nylon film (1.59 mM) by Fatarella et al The maximum value of 4.7 Mm was found for Amberlit IR‐120H by Spinelli et al, which was a result of the diffusion limitation of the substrate due to interactions between the substrate and the support, or due to the conformational change of the enzyme with lower possibility to form the substrate‐enzyme complex …”

Section: Resultsmentioning

confidence: 92%

“…Additionally, the porous loofa carrier material provides a large surface area, which enables a large number of laccase molecules to immobilize in close proximity covering the entire surface and preventing the enzyme deactivation. Fatarella et al also found that the physical properties of the carrier (e.g. shape, surface area, and pore diameter) influence the enzyme binding as well as the activity, reusability, and even stability of the immobilized enzyme.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic decolourization of water using loofa sponge as cellular carrier: Immobilization and dye degradation performance

et al. 2018

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In recent years, strong efforts have been made to develop sustainable biocatalytic decolourization processes for dye‐polluted water. In particular, dye‐oxidizing laccase enzymes immobilized on suitable carriers are promising candidates, and can be reused as long as the activity is sufficiently high. In this work, we propose, for the first time, a new methodology to immobilize laccase from Trametes hirsute on a naturally‐grown and decomposable cellular loofa sponge carrier and assess the capability to degrade dye‐polluted water. High immobilization activity is achieved and about 70 % residual activity remains after 8 cycles. Additionally, we determined homogenous and heterogeneous kinetic parameters for free and immobilized enzymes. The results reveal a four times higher Michaelis‐Menten constant of the laccase immobilized on the loofa due to mass transfer and mixing limitations in a packed bed bio‐reactor. The response surface methodology was applied to identify favourable operating conditions. In particular, dye concentration and treatment time determine the decolourization performance, while mixing velocity has only a minor effect. Eventually, the results demonstrated a remarkable dye removal capability with shorter treatment time compared to the previous studies on immobilized laccase reported in the literature.

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Nylon 6 film and nanofiber carriers: Preparation and laccase immobilization performance

Cited by 64 publications

References 38 publications

Electrospun nanofibrous membranes for solid‐phase extraction of estriol from aqueous solution

Electrospun nanofibrous membranes for solid‐phase extraction of estriol from aqueous solution

Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres

Enzymatic decolourization of water using loofa sponge as cellular carrier: Immobilization and dye degradation performance

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