Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform

Pikó, Péter; Pajor, László; Szűcs, Sándor; Kósa, Zsigmond; Sándor, János; Ádány, Róza

doi:10.3390/biom11020326

Cited by 30 publications

(26 citation statements)

References 54 publications

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“…Studies revealed that LPC was strongly associated with obesity. In particular, obese individuals have low plasma LPC concentrations [53][54][55] , and there is indication that effective and long-term weight loss may restore LPC to normal levels 56 . In case of infancy and childhood overweight/obesity, LPC is strongly related to rapid growth.…”

Section: Discussionmentioning

confidence: 99%

Metabolic signatures in human follicular fluid identify lysophosphatidylcholine as a predictor of follicular development

Yang

et al. 2022

Commun Biol

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In order to investigate the metabolic characteristics of human follicular fluid (FF) and to reveal potential metabolic predictors of follicular development (FD) with clinical implications, we analyzed a total of 452 samples based on a two-stage study design. In the first stage, FF samples from both large follicles (LFs) and matched-small follicles (SFs) of 26 participants were analyzed with wide-spectrum targeted metabolomics. The metabolic signatures were described by multi-omics integration technology including metabolomic data and transcriptomic data. In the second stage, the potential biomarkers of FD were verified using enzyme-linked immunoassay with FF and blood serum from an independent 200 participants. We describe the FF metabolic signatures from ovarian follicles of different developmental stages. Lysophosphatidylcholine (LPC) can be used as a biomarker of FD and ovarian sensitivity, advancing the knowledge of metabolic regulation during FD and offering potential detection and therapeutic targets for follicle and oocyte health improvements in humans.

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Section: Discussionmentioning

confidence: 99%

Metabolic signatures in human follicular fluid identify lysophosphatidylcholine as a predictor of follicular development

Yang

et al. 2022

Commun Biol

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“…Altogether, plasma lipid profiling of ob/ob mice confirmed circulating lipid biomarkers of obesity. Significantly altered lipid moieties in obese mice plasma recapitulated global obesity-related changes in human plasma lipidome (30, 33–35), reinforcing the relevance of using the ob/ob mice as a preclinical model of obesity.…”

Section: Resultsmentioning

confidence: 70%

“…It is no longer restricted by copyright. Anyone can legally obesity-related changes in human plasma lipidome (30,(33)(34)(35), reinforcing the relevance of using the ob/ob mice as a preclinical model of obesity.…”

Section: Lipidomic Analysis Of Obese Mice Plasma Reflects Common Lipid Alterations Associated With Obesitymentioning

confidence: 95%

Lipidomic analysis of adipose-derived extracellular vesicles reveals their potential as lipid mediators of obesity-associated metabolic complications

Blandin

Hilairet

Ponnaiah

et al. 2021

Preprint

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Adipose extracellular vesicles (AdEV) transport lipids that could participate to the development of obesity-related metabolic dysfunctions. This study aimed to define mice AdEV lipid signature in either healthy or obesity context by a targeted LC-MS/MS approach. Distinct clustering of AdEV and visceral adipose tissue (VAT) lipidomes by principal component analysis reveals specific lipid composition of AdEV compared to source VAT. Comprehensive analysis identifies enrichment of ceramides and phosphatidylglycerols in AdEV compared to VAT in lean conditions. Lipid subspecies commonly enriched in AdEV highlight specific AdEV-lipid sorting. Obesity impacts AdEV lipidome, driving triacylglycerols and sphingomyelins enrichment in obese versus lean conditions. Obese mice AdEV also display elevated phosphatidylglycerols and acid arachidonic subspecies contents highlighting novel biomarkers and/or mediators of metabolic dysfunctions. Our study identifies specific lipid-fingerprints for plasma, VAT and AdEV that are informative of the metabolic status and underline the signaling capacity of lipids transported by AdEV in obesity-associated complications.

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“…However, several other phospholipid biomarkers provided insight into the health of the mice. Serum LPC concentrations are typically inversely associated with obesity [ 58 ], and specifically, LPC 18 : 2 is inversely associated with childhood obesity, BMI, and metabolic risk factors in humans [ 48 , 58 , 59 ]. Conversely, recent research with murine livers indicates that greater liver LPC concentrations are associated with NAFLD [ 49 ], but inversely associated with NASH [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Age- and Diet-Dependent Changes in Hepatic Lipidomic Profiles of Phospholipids in Male Mice: Age Acceleration in Cyp2b-Null Mice

et al. 2022

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Increases in traditional serum lipid profiles are associated with obesity, cancer, and cardiovascular disease. Recent lipidomic analysis has indicated changes in serum lipidome profiles, especially in regard to specific phosphatidylcholines, associated with obesity. However, little work has evaluated murine hepatic liver lipidomic profiles nor compared these profiles across age, high-fat diet, or specific genotypes, in this case the lack of hepatic Cyp2b enzymes. In this study, the effects of age (9 months old), high-fat diet (4.5 months old), and the loss of three primarily hepatic xeno- and endobiotic metabolizing cytochrome P450 (Cyp) enzymes, Cyp2b9, Cyp2b10, and Cyp2b13 (Cyp2b-null mice), on the male murine hepatic lipidome were compared. Hierarchical clustering and principal component analysis show that age perturbs hepatic phospholipid profiles and serum lipid markers the most compared to young mice, followed by a high-fat diet and then loss of Cyp2b. Several lipid biomarkers such as PC/PE ratios, PE 38 : 6, and LPC concentrations indicate greater potential for NAFLD and hypertension with mixed effects in Cyp2b-null mice(less NAFLD and greater hypertension-associated markers). Lipid profiles from older mice contain greater total and n-6 fatty acids than normal diet (ND)-fed young mice; however, surprisingly, young Cyp2b-null mice contain high n-6 : n-3 ratios. Overall, the lack of Cyp2b typically enhanced adverse physiological parameters observed in the older (9 mo) mice with increased weight gain combined with a deteriorating cholesterol profile, but not necessarily all phospholipid profiles were adversely perturbed.

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Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform

Cited by 30 publications

References 54 publications

Metabolic signatures in human follicular fluid identify lysophosphatidylcholine as a predictor of follicular development

Metabolic signatures in human follicular fluid identify lysophosphatidylcholine as a predictor of follicular development

Lipidomic analysis of adipose-derived extracellular vesicles reveals their potential as lipid mediators of obesity-associated metabolic complications

Age- and Diet-Dependent Changes in Hepatic Lipidomic Profiles of Phospholipids in Male Mice: Age Acceleration in Cyp2b-Null Mice

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