Observations on the photo‐electric estimation of total hæmoglobin

Reeve, E. B.

doi:10.1002/path.1700560112

J. Pathol.

1944

DOI: 10.1002/path.1700560112

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Observations on the photo‐electric estimation of total hæmoglobin

E. B. Reeve

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1945

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Cited by 5 publications

(2 citation statements)

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“…Although these three methods seem to be the most favoured they are moderately complicated, and the latter two use dangerous or objectionable chemicals. The use of oxyhaemoglobin, the most easily prepared derivative, has been suggested by Szigeti (1940), Karr & Clark (1941), Bell & Guthmann (1943) and Reeve (1944). This paper describes our experiences with the oxyhaemoglobin method using the balanced modification of the photoelectric colorimeter described by Bell & Guthmann (1943).…”

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confidence: 96%

The routine estimation of haemoglobin as oxyhaemoglobin

Bell

Chambers

Waddell

1945

Biochemical Journal

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mentioning

confidence: 96%

The routine estimation of haemoglobin as oxyhaemoglobin

Bell

Chambers

Waddell

1945

Biochemical Journal

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“…The concentrations of dye in plasma after a single injection of dye were of the order of 1 mg./100 ml., and the dye was a sample of T 1824 obtained from Imperial Chemical Industries. Estimations were made by a photoelectric photometer of the type described by Reeve (1944), in both methods with the red filters used by…”

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confidence: 99%

Observations on plasma volume estimations with the dye T 1824

Reeve¹,

Armin²

1946

The Journal of Physiology

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During work on battle casualties in the last 18 months of the campaign of the allied armies in Italy, it was particularly desired to relate the clinical states of casualties seen at various times after injury to their blood volumes at those times, and more than 200 blood-volume estimations were made with the dye T 1824. At the times at which it was desired to know the blood volume, a proportion of these casualties had, in the preceding 5 hr., had doses of 16 mg. (gr. 1/4) and sometimes of 32 mg. of morphine, and some had received proprietary mixtures of papaverine and hyoscine. Bowler, Crooke & Morris (1944) have reported that if blood-volume determinations are made with the dye T 1824 after patients have received injections of morphine or hyoscine or both, considerable fluctuations in the concentration of dye are found in a series of samples drawn over a period of several hours after the injection of dye. To estimate the plasma volume from the dye content of plasma samples, one may use either the dye content of a single sample of plasma drawn several minutes after the injection of dye, at a time at which it is assumed that the dye is properly mixed with the plasma and there has been negligible loss of dye from the circulating plasma; or an attempt may be made to correct for loss of dye by estimating the dye contents of a series of samples drawn at varying times after the injection of dye, and extrapolating through these values to zero time. The claims of Bowler et al. on the effects of morphine and hyoscine throw doubt on the validity of blood volumes estimated by the single sample method in patients who have received morphine or hyoscine, and from their published figures it can be seen that extrapolation through the values of dye concentration, obtained in their subjects who received morphine and hyoscine, may be impossible.According to these authors fluctuations in dye disappearance curves are obtained if the dye is injected froni 20 min. to 5 hr. after the injection of a mixture of 16-2 mg. morphine sulphate, 0-65 mg. hyoscine hydrobromide and 0-65 mg. atropine sulphate, or after the injection of morphine (dose not stated) or hyoscine hyarobromide (dose not stated), but not after atropine sulphate * Work undertaken on behalf of the Director-General of the Army Medical Services and the Medical Research Council.

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The use oe dilute alkali in haemoglobinometry

Barer¹,

Gaffney²

1957

Clinica Chimica Acta

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Observations on the photo‐electric estimation of total hæmoglobin

Cited by 5 publications

References 9 publications

The routine estimation of haemoglobin as oxyhaemoglobin

The routine estimation of haemoglobin as oxyhaemoglobin

Observations on plasma volume estimations with the dye T 1824

The use oe dilute alkali in haemoglobinometry

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