2020
DOI: 10.1590/1809-4392201901341
|View full text |Cite
|
Sign up to set email alerts
|

Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

Abstract: The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
10
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
2

Relationship

1
1

Authors

Journals

citations
Cited by 2 publications
(10 citation statements)
references
References 36 publications
0
10
0
Order By: Relevance
“…After growth, fragments of 3 × 3 mm from the edge of the cultures were inoculated in the center of new Petri dishes with equal volume and content. Then they were incubated under the conditions suggested by Santana et al (2020b) to obtain the pure culture. Thus, each species was considered a treatment, totaling six treatments.…”
Section: Fungal Strainsmentioning
confidence: 99%
See 4 more Smart Citations
“…After growth, fragments of 3 × 3 mm from the edge of the cultures were inoculated in the center of new Petri dishes with equal volume and content. Then they were incubated under the conditions suggested by Santana et al (2020b) to obtain the pure culture. Thus, each species was considered a treatment, totaling six treatments.…”
Section: Fungal Strainsmentioning
confidence: 99%
“…For the discoloration test of the blue trypan dye in solid medium, a 3 × 3 mm block was used, containing mycelium removed from the edge of the pure strain of each species and transferred to the centre of a Petri dish (90 mm in diameter) containing 15 mL of the homogeneous mixture of PDA plus trypan blue (0.05%). Petri dishes were incubated according to the indications of Santana et al (2020b), and each plate was considered a replicate. Every three days, from the beginning of the experiment, for a period of 21 d of incubation, the dye discoloration halo was evaluated by measuring the diameter with a ruler (orthogonal directions).…”
Section: Trypan Blue Dye Discoloration In Solid Culture Mediummentioning
confidence: 99%
See 3 more Smart Citations