Currently, the literature data regarding the concentration of microelements in walnut oil are low, most of them being related to their presence in the walnut core. The purpose of this study was to determine the distribution of essential and toxic mineral elements from walnut oil marketed in Timisoara (Romania) and to evaluate the mineral contribution of this important food in the prevention of cardiovascular diseases. The paper presents the results obtained in determining the content of some essential and toxic microelements of the native walnut oil, used as dietary food. The results obtained from the analysis of the essential and toxic mineral elements, by Inductively Coupled Plasma - Mass Spectrometer (ICP-MS) method, reveal that the walnut oil samples analyzed contain noticeable amounts of: Fe (2,576 - 3,621 mg/kg), Mn (1,876 - 1,783 mg/kg), Zn (1,120 - 1,782 mg/kg), low Cu (0.185 - 0.226 mg/kg), Cr (0.088 - 0.154 mg/kg) and Ni (0.052 - 0.081 mg/kg) and very low Co (0.001 - 0.003 mg / kg). Pb and Cd, elements with pronounced toxic potential, were identified in very low concentrations: 0.020 - 0.031 mg/kg Pb, respectively 0.001 - 0.002 mg /kg Cd. The results obtained when evaluating the mineral intake show that, under the conditions of this experiment, a daily consumption of 30 g of walnut oil cannot be considered as the only additional source of microelements.