Occurrence and Genomic Characterization of ESBL-Producing, MCR-1-Harboring Escherichia coli in Farming Soil

Zheng, Beiwen; Huang, Can; Xu, Hao; Guo, Lan-Ping; Zhang, Jing; Wang, Xin; Jiang, Xin; X, Yu; Jin, Liu; Li, Xuewen; Feng, Youjun; Xiao, Yonghong; Li, Lanjuan

doi:10.3389/fmicb.2017.02510

Cited by 58 publications

(35 citation statements)

References 44 publications

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“…Additionally, this gene has been detected in bovine and horse manure [98]. Livestock manure seems to be an important reservoir of mcr-encoding plasmids, mostly carried by colistin-resistant Escherichia coli on diverse plasmid replicon types: IncX4, IncI1, IncFII, IncFIB, IncX1 and IncQ1 [97,99,100].…”

Section: Antibiotics and Antimicrobial Resistance Genes In Manurementioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

140

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The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

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Section: Antibiotics and Antimicrobial Resistance Genes In Manurementioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

140

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“…Plasmids of the incompatibility group HI2 (IncHI2) have been linked with the carriage of tellurium resistance, plus a broad range of antimicrobial resistance genes, including bla IMP, bla NDM, and bla VIM (carbapenemase resistance) (Abraham et al, 2016;Dolejska et al, 2016;Falgenhauer et al, 2017), bla CTX−M (Garcia Fernandez et al, 2007), mcr-1 (plasmid-mediated colistin resistance) (Gilrane et al, 2017;Li et al, 2017;Zheng et al, 2017), oqxAB and aac(6')-Ib-cr (plasmid-mediated quinolone resistance) (Fang et al, 2016), and resistance genes effective against first generation antibiotics and disinfectants (Cain et al, 2010;Cain and Hall, 2012;Abraham et al, 2016;Dolejska et al, 2016;Gilrane et al, 2017). Notably, clinically-important resistance genes have been associated with IncHI2 plasmids that also encode resistance to copper, zinc and, arsenic residues (Fang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Complete Sequences of Multiple-Drug Resistant IncHI2 ST3 Plasmids in Escherichia coli of Porcine Origin in Australia

Wyrsch

Reid

DeMaere

et al. 2019

Front. Sustain. Food Syst.

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IncHI2 ST3 plasmids are known carriers of multiple antimicrobial resistance genes. Complete plasmid sequences from multiple drug resistant Escherichia coli circulating in Australian swine is however limited. Here we sequenced two related IncHI2 ST3 plasmids, pSDE-SvHI2, and pSDC-F2_12BHI2, from phylogenetically unrelated multiple-drug resistant Escherichia coli strains SvETEC (CC23:O157:H19) and F2_12B (ST93:O7:H4) from geographically disparate pig production operations in New South Wales, Australia. Unicycler was used to co-assemble short read (Illumina) and long read (PacBio SMRT) nucleotide sequence data. The plasmids encoded three drug-resistance loci, two of which carried class 1 integrons. One integron, hosting drfA12-orfF-aadA2, was within a hybrid Tn1721/Tn21, with the second residing within a copper/silver resistance transposon, comprising part of an atypical sul3-associated structure. The third resistance locus was flanked by IS15DI and encoded neomycin resistance (neoR). An oqx-encoding transposon (quinolone resistance), similar in structure to Tn6010, was identified only in pSDC-F2_12BHI2. Both plasmids showed high sequence identity to plasmid pSTM6-275, recently described in Salmonella enterica serotype 1,4,[5],12:i:-that has risen to prominence and become endemic in Australia. IncHI2 ST3 plasmids circulating in commensal and pathogenic E. coli from Australian swine belong to a lineage of plasmids often in association with sul3 and host multiple complex antibiotic and metal resistance structures, formed in part by IS26.

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“…5 In our previous study, we isolated six mcr-1-positive ESBL-producing E. coli strains from farm soils. 6 Wastewater treatment plants (WWTPs), which have been taken as deep reservoirs of ARGs and antimicrobial-resistant bacteria (ARB), are believed to offer the opportunity for ARGs to flow into susceptible disease-causing bacteria. 7,8 Thus, the surveillance of these environment-derived bacteria that carry the mcr-1 gene with other types of ARGs is of great importance and the transmission patterns of these bacteria between different habitats should be elucidated.…”

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confidence: 99%

Co-Existence of mcr-1 and blaNDM-5 in an Escherichia coli Strain Isolated from the Pharmaceutical Industry, WWTP

Han¹,

Liu²,

Cui³

et al. 2020

IDR

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The emergence of the plasmid-borne colistin-resistant gene (mcr-1) poses a great threat to human health. What is worse, the recent observations of the coexistence of mcr-1 with other antimicrobial resistance genes in some bacteria cause further concern. Here, we present the first report of a wild Escherichia coli strain that co-carries an mcr-1 encoding phage-like IncY plasmid (pR15_MCR-1) and a bla NDM-5 encoding IncX3 plasmid (pR15_NDM-5) from a pharmaceutical industry, wastewater treatment plant, in China. This study highlights the spreading of E. coli carrying both mcr-1 and bla NDM-5 genes in the pharmaceutical industry. Importance: Escherichia coli strains that carry both mcr-1 and bla NDM-5 genes are of great health concern and are already found in humans and animals worldwide, yet there is a paucity of observations of this resistant strain in the environment. Here we present the first isolation of an E. coli strain (R15) that co-carries mcr-1 and bla NDM-5 genes from a wastewater treatment plant in China. Whole-genome sequencing indicated that R15 harbored two plasmids, pR15_MCR-1 and pR15_NDM-5, that carry mcr-1 and bla NDM-5 , respectively. The observation of this wild-derived E. coli strain that carries mcr-1 and bla NDM-5 genes simultaneously calls for the urgency to improve monitoring and reducing its further spreading.

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Occurrence and Genomic Characterization of ESBL-Producing, MCR-1-Harboring Escherichia coli in Farming Soil

Cited by 58 publications

References 44 publications

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Complete Sequences of Multiple-Drug Resistant IncHI2 ST3 Plasmids in Escherichia coli of Porcine Origin in Australia

<p>Co-Existence of <em>mcr-1</em> and <em>bla</em><sub>NDM-5</sub> in an <em>Escherichia</em> <em>coli</em> Strain Isolated from the Pharmaceutical Industry, WWTP</p>

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