Occurrence, Ecology and Phylogeny of Banana Streak Badnavirus (Bsv) and Cucumber Mosaic Cucumovirus (Cmv) in Musa Sp. Production Areas of the Mediterranean Coastline of Turkey

Fidan, Hakan; Koç, Gökmen

doi:10.15666/aeer/1703_59355951

Cited by 6 publications

(10 citation statements)

References 12 publications

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“…Mixed viral infections are frequent in perennial and vegetative plants. Likewise, the high prevalence of mixed infection of CMV with BBTV or BSV, which was also examined by serological tests and PCR-based methods ( Fidan & Koc, 2019 ; Liu et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…In banana plants, BBTV and CMV are mainly transmitted by vector insects, such as aphids. CMV infection is found in diverse hosts, not only in cultivated vegetables such as melon, pepper, tomato, and squash, but also in bananas through aphid transmission ( Fidan & Koc, 2019 ). Further, BSV infection in bananas could occur through transmission by mealybugs in the field.…”

Section: Discussionmentioning

confidence: 99%

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Development of a bead-based assay for detection of three banana-infecting viruses

Kuan

Tsai²,

Tseng

et al. 2022

PeerJ

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Background Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana production in banana-growing areas. Methods A multiplex polymerase chain reaction (PCR) protocol combined with LiquiChip analysis to identify BSV, BBTV, and CMV, with consistent amplification of plant ubiquitin (UBQ), the banana plant messenger RNA used as a procedural control. Multiplex reverse transcription (RT)-PCR amplicons were extended by allele-specific primers, followed by hybridization with carboxylated microspheres containing unique fluorescent oligonucleotides, which were detected using the LiquiChip 200 workstation. Results In this study, we aimed to develop a rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay that can be applied in routine diagnosis. We demonstrated that this detection system was extremely efficient and highly specialized for differentiating individual in a mixture of viruses while being ten times more sensitive than traditional RT-PCR. The development of this method makes it feasible to detect banana viruses in field collected leaf samples.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of a bead-based assay for detection of three banana-infecting viruses

Kuan

Tsai²,

Tseng

et al. 2022

PeerJ

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“…A new degenerate, inosine-containing diagnostic primer was designed to improve the inclusiveness of the detection protocol, which failed to detect these novel isolates. In fact, this kind of primer could help to overcome the high genetic diversity of the virus, even if a lower sensitivity could sometimes result from the degeneracy and inosine contents of the primers [ 9 , 13 , 14 ]. The new primer detected BanMMV infection in two samples that tested negative with the existing primer, but failed to detect three infections among the 110 tested accessions.…”

Section: Discussionmentioning

confidence: 99%

“…‘Pisang Awak’, ABB genome). BanMMV often occurs as a mixed infection with other banana-infecting viruses such as banana streak viruses (BSV) and cucumber mosaic virus (CMV), and the mosaic symptoms of BanMMV may be masked by those of the other viruses [ 1 , 13 , 14 ]. On the other hand, previous studies reported that, in Guadeloupe, infection by BanMMV exacerbated the symptoms of CMV causing leaf necrosis symptoms compared with mosaic symptoms from CMV infection alone [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Divergent Isolates of Banana Mild Mosaic Virus and Development of a New Diagnostic Primer to Improve Detection

et al. 2020

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Banana mild mosaic virus (BanMMV) (Betaflexiviridae, Quinvirinae, unassigned species) is a filamentous virus belonging to the Betaflexiviridae family. It infects Musa spp. with a very wide geographic distribution. The genome variability of plant viruses, including the members of the Betaflexiviridae family, makes their molecular detection by specific primers particularly challenging. During routine indexing of the Musa germplasm accessions, a discrepancy was observed between electron microscopy and immunocapture (IC) reverse transcription (RT) polymerase chain reaction (PCR) test results for one asymptomatic accession. Filamentous viral particles were observed while molecular tests failed to amplify any fragment. The accession underwent high-throughput sequencing and two complete genomes of BanMMV with 75.3% of identity were assembled. Based on these sequences and on the 54 coat protein sequences available from GenBank, a new forward primer, named BanMMV CP9, compatible with Poty1, an oligodT reverse primer already used in diagnostics, was designed. A retrospective analysis of 110 different germplasm accessions from diverse origins was conducted, comparing BanMMCP2 and BanMMV CP9 primers. Of these 110 accessions, 16 tested positive with both BanMMCP2 and BanMMV CP9, 3 were positive with only BanMMCP2 and 2 tested positive with only BanMMV CP9. Otherwise, 89 were negative with the two primers and free of flexuous virions. Sanger sequencing was performed from purified PCR products in order to confirm the amplification of the BanMMV sequence for the five accessions with contrasting results. It is highly recommended to use the two primers successively to improve the inclusiveness of the protocol.

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“…The period of latency of the virus to showcase symptoms in the infected plants is not only a threat to the domestic market especially where it is vegetatively propagated but also to international germplasm exchange [20]. The tissue culture planting materials that look prima facie healthy, especially have to be indexed for the presence of this virus [21].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Coat Protein of Banana Bract Mosaic Virus as a Potential Antigen for Serological Detection of the Virus

Dilip

Louis

Sabharwal

et al. 2020

CJAST

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Banana bract mosaic disease caused by Banana bract mosaic potyvirus (BBrMV) is reported to instigate heavy loss in banana and plantain across Asia. Almost all the cultivars of banana succumb to the disease resulting in malformed bunches weighing less than half of normal ones. In the current study the coat protein (CP) gene segment present at the 3’ terminal region of the viral genome amplified by RT-PCR was cloned into expression vectors, pRSET-C and pGEX-4T-2 to use it for raising polyclonal antiserum which in turn will aid in developing assays to detect the virus. Recombinant BBrMV CP (rCP) in pRSET-C when expressed was insoluble whereas, it was in the soluble fraction when expressed from pGEX-4T-2. The GST-fusion protein was purified by GSH sepharose affinity column chromatography and western blot analysis was performed using anti GST antibodies. 360 µg/ml of protein was purified from 1 l of culture. The GST tag was cleaved from the purified protein by incubation with thrombin at 25°C overnight. The rCP was characterized using ultracentrifugation, fluorescence spectroscopy and electron microscopy. The tagless monomer failed to assemble to virus like particles (VLPs) in vitro which was substantiated by fluorescence spectroscopy. This study will be first step towards deciphering structure and functions of Banana bract mosaic virus coat protein.

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Occurrence, Ecology and Phylogeny of Banana Streak Badnavirus (Bsv) and Cucumber Mosaic Cucumovirus (Cmv) in Musa Sp. Production Areas of the Mediterranean Coastline of Turkey

Abstract: Occurrence, ecology and phylogeny of banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV) in Musa sp. production areas of the Mediterranean coastline of Turkey -5935 -

Cited by 6 publications

References 12 publications

Development of a bead-based assay for detection of three banana-infecting viruses

Development of a bead-based assay for detection of three banana-infecting viruses

Identification of Divergent Isolates of Banana Mild Mosaic Virus and Development of a New Diagnostic Primer to Improve Detection

Recombinant Coat Protein of Banana Bract Mosaic Virus as a Potential Antigen for Serological Detection of the Virus

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