Occurrence of Peritrophic Membranes

Peters, Werner

doi:10.1007/978-3-642-84414-0_2

Cited by 33 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, no staining occurred with LFA which argues against the presence of neuraminic acid in the PM. Chitin is believed to be a constituent universally present in PMs (Peters 1992). The presence of chitin in all three layers of the PM of G. m. morsitans is consistent with the binding of WGA and LEA to the PM ( Table 2).…”

Section: Resultsmentioning

confidence: 61%

“…Similar phenomena have been observed in other tissues (Michael et al 1970;Faraggiana and Grishman 1981;Goode et al 1991) and it has been suggested that, under the influence of pH, the structural configuration of the molecules involved alters, thus changing the number of anionic sites available for cationic labelling. Given our general knowledge of the chemical composition of the PM (Peters 1992) charge sites visible at pH 3 and above but not at pH 2 are probably due to carboxyl groups. However, the above argument for changes in structural configuration with changing pH, with the possibilities that entail for the masking or exposure of sulphated groups, must be borne in mind.…”

Section: Discussionmentioning

confidence: 98%

“…Sulphate, carboxyl and phosphates are the common anionic groups in biological material. Phosphate groups are usually associated with membrane phospholipids or nucleic acids neither of which are likely constituents of PM (Peters 1992). Carboxyl groups are commonly found in GAGs and glycoproteins and some other sugar-containing structures, while sulphate groups are normally confined to GAGs.…”

Section: Discussionmentioning

confidence: 99%

“…Peritrophic matrices (PM) are non-cellular, glycosaminoglycan (GAG)-containing, often multilaminate cylindrical sheets separating the food (and any parasites contained) from the midgut epithelium (Peters 1992;Binnington et al 1995). It is clear that the PM forms a filter during the physiological processes of digestion and is a barrier to parasite invasion, but details of PM function remain largely unknown.…”

Section: Introductionmentioning

confidence: 98%

“…A fuller understanding of these roles requires a better understanding of the chemical composition of the PM. The remarkably small amount of data currently available on PM chemical composition is reviewed by Peters (1992). To generalise, PMs have a substantial protein component (Ramos et al 1994) and are rich in sugar residues, probably because of the presence of glycoproteins, GAG-containing proteoglycans and structural polysaccharides such as chitin.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Composition of the peritrophic matrix of the tsetse fly, Glossina morsitans morsitans

Lehane

Allingham

Weglicki

1996

Cell and Tissue Research

View full text Add to dashboard Cite

The three-layered peritrophic matrix of Glossina morsitans morsitans is shown, by histochemistry, to be formed of a mixture of glycosaminoglycans, glycoproteins and chitin. In all three layers the glycosaminoglycans contain GlcNAc-hexuronic and Gal-GlcNAc moieties, together with chitin. Glycosaminoglycans in layer 3 are sulphated and sulphated sites have a mean interspace distance of 53 nm - similar to the spacing of fixed charge sites in glomerular basement membrane suggesting a rôle for these sites in the filtration properties of the peritrophic matrix. O-linked oligo- saccharides are present in all three layers. Layer 1 contains the widest variety of glycoprotein oligosaccharide constructs GlcNac and alphalinked GalNAc possibly as GalNAcalpha1,3GalNAc, the latter apparently distal to Galbeta1-4GlcNAc. Lectin binding suggests that layer 2 contains GalNAcalpha1,3Galbeta1,3GlcNAc and that layer 3 contains GalNAc and Galbeta1,4GlcNAc. The evidence for N-linked oligosaccharides is more equivocal. Two dimensional electrophoresis showed that the peritrophic matrix contains a range of proteins, most of which require relatively harsh treatment for their solubilization.

show abstract

Section: Resultsmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Composition of the peritrophic matrix of the tsetse fly, Glossina morsitans morsitans

Lehane

Allingham

Weglicki

1996

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

REGULATION OF AMYLASE, CELLULASE AND CHITINASE SECRETION IN THE DIGESTIVE TRACT OF THE TWO‐SPOTTED FIELD CRICKET, Gryllus bimaculatus

Weidlich

Müller

Hoffmann

et al. 2013

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The secretion of amylase and cellulase in Gryllus bimaculatus is determined by increased food intake, whereby shortly after molting food consumption increases. About half of the standing amylase concentration (activity) in the endothelial cells can be secreted within 30 min. The peak of amylase and cellulase secretion that occurs in the photophase is related to the feeding peak in the previous scotophase. The secretion of chitinase on the other hand is primarily controlled by the molting cycle. Only amylase secretion was affected by calcium in the incubation medium, suggesting an apocrine release mechanism. Refeeding experiments (after 5 days without food) suggest that the release of amylase in response to a nutrient in the lumen (glucose) is not due to simple stimulation of exocytosis, but rather a stimulation of synthesis.

show abstract

Chitin deacetylase: A potential target for Mythimna separata (Walker) control

Liu

Yang

Yao³

et al. 2020

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Chitin deacetylase (CDA) is a hydrolytic enzyme that modifies chitin into chitosan in the body of insects. In this study, we obtained a full‐length complementary DNA sequence (MsCDA1) from the oriental armyworm Mythimna separata by high‐throughput sequencing. MsCDA1 is 1,952 bp long and includes 1,620 bp open reading frame encoding 539 amino acids. Analysis by quantitative real time polymerase chain reaction showed that MsCDA1 expression was higher at the adult stage than at earlier developmental stages. MsCDA1 was expressed in all larval tissues examined, in which the highest expression level was found in the midgut. The RNA interference (RNAi) suppressed MsCDA1 expression levels at 12, 24, and 48 hr after injection of double‐stranded RNA (1–4 μg per larva) specific to MsCDA1. Under RNAi condition, CDA enzyme activity was significantly reduced and changes an ultramicroscopic structure of M. separata peritrophic matrix especially in its microfibrillar organization exhibiting loose network. In contrast, the surface of the peritrophic matrix was relatively smooth and well organized at control or low RNAi conditions. Moreover, RNAi of MsCDA1 expression impaired larval growth and development, occasionally leading to larval death. These results demonstrate that MsCDA1 plays a crucial role in maintaining peritrophic matrix integrity in M. separata.

show abstract

Occurrence of Peritrophic Membranes

Cited by 33 publications

References 0 publications

Composition of the peritrophic matrix of the tsetse fly, Glossina morsitans morsitans

Composition of the peritrophic matrix of the tsetse fly, Glossina morsitans morsitans

REGULATION OF AMYLASE, CELLULASE AND CHITINASE SECRETION IN THE DIGESTIVE TRACT OF THE TWO‐SPOTTED FIELD CRICKET, Gryllus bimaculatus

Chitin deacetylase: A potential target for Mythimna separata (Walker) control

Contact Info

Product

Resources

About