Ocena odsetka limfocytów CD19+CD5+ w przerosłych migdałkach gardłowych u dzieci chorych na wysiękowe zapalenie ucha środkowego

Skotnicka, Bożena; Kasprzycka, Edwina; Żelazowska-Rutkowska, Beata; Wysocka, Jolanta; Anisimowicz, Sławomir

doi:10.1016/s0030-6657(07)70561-9

Cited by 10 publications

(4 citation statements)

References 15 publications

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“…It has been shown that the accelerated apoptosis of PBMCs adversely affects the immune system, causing depressed human immunity [41], which may finally lead to autoimmune diseases, such as type 1 diabetes, allergy, or asthma, as well as cancer development [42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Apoptosis-Inducing Potential of Selected Bromophenolic Flame Retardants 2,4,6-Tribromophenol and Pentabromophenol in Human Peripheral Blood Mononuclear Cells

2022

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(1) Background: 2,4,6-Tribromophenol (2,4,6-TBP) and pentabromophenol (PBP) are utilized as brominated flame retardants (BFRs) in order to reduce the combustion of materials used in various utility products. The presence of 2,4,6-TBP and PBP has been reported in environmental samples as well as in inhaled air, dust, food, drinking water, and the human body. To date, there are limited data concerning the toxic action of 2,4,6-TBP and particularly PBP, and no study has been conducted to assess the apoptotic mechanism of action of these substances in human leukocytes. (2) Methods: PBMCs were isolated from leukocyte–platelet buffy coat and treated with tested substances in concentrations ranging from 0.01 to 50 µg/mL for 24 h. The apoptotic mechanism of action of the tested BFRs was assessed by the determination of phosphatidylserine exposure on the PBMCs surface, the evaluation of mitochondrial potential and cytosolic calcium ion levels, and the determination of caspase-8, -9, and -3 activation. Moreover, poly (ADP-ribose) polymerase-1 (PARP-1) cleavage, DNA fragmentation, and chromatin condensation were analyzed. (3) Results: 2,4,6-TBP and, more strongly, PBP induced apoptosis in PBMCs, changing all tested parameters. It was also found that the mitochondrial pathway was mainly involved in the apoptosis of PBMCs exposed to the studied compounds. (4) Conclusions: 2,4,6-TBP and PBP triggered apoptosis in human PBMCs, and some observed changes occurred at 2,4,6-TBP concentrations that were detected in humans occupationally exposed to this substance.

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Section: Introductionmentioning

confidence: 99%

Apoptosis-Inducing Potential of Selected Bromophenolic Flame Retardants 2,4,6-Tribromophenol and Pentabromophenol in Human Peripheral Blood Mononuclear Cells

2022

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“…It was shown that accelerated apoptosis of PBMCs may be associated with adverse changes in the immune system, such as depleted human immunity [39], which, in consequence, may lead to the development of autoimmune diseases (type 1 diabetes, asthma, allergy) and cancer [40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Determination of Apoptotic Mechanism of Action of Tetrabromobisphenol A and Tetrabromobisphenol S in Human Peripheral Blood Mononuclear Cells: A Comparative Study

2022

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Background: Tetrabromobisphenol A (TBBPA) is the most commonly used brominated flame retardant (BFR) in the industry. TBBPA has been determined in environmental samples, food, tap water, dust as well as outdoor and indoor air and in the human body. Studies have also shown the toxic potential of this substance. In search of a better and less toxic BFR, tetrabromobisphenol S (TBBPS) has been developed in order to replace TBBPA in the industry. There is a lack of data on the toxic effects of TBBPS, while no study has explored apoptotic mechanism of action of TBBPA and TBBPS in human leukocytes. Methods: The cells were separated from leucocyte-platelet buffy coat and were incubated with studied compounds in concentrations ranging from 0.01 to 50 µg/mL for 24 h. In order to explore the apoptotic mechanism of action of tested BFRs, phosphatidylserine externalization at cellular membrane (the number of apoptotic cells), cytosolic calcium ion and transmembrane mitochondrial potential levels, caspase-8, -9 and -3 activation, as well as PARP-1 cleavage, DNA fragmentation and chromatin condensation in PBMCs were determined. Results: TBBPA and TBBPS triggered apoptosis in human PBMCs as they changed all tested parameters in the incubated cells. It was also observed that the mitochondrial pathway was mainly involved in the apoptotic action of studied compounds. Conclusions: It was found that TBBPS, and more strongly TBBPA, triggered apoptosis in human PBMCs. Generally, the mitochondrial pathway was involved in the apoptotic action of tested compounds; nevertheless, TBBPS more strongly than TBBPA caused intrinsic pathway activation.

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“…Different degrees of airway obstruction may lead to the development of an abnormal pattern of breathing through the mouth (oral respiratory pattern). Impaired ventilation and exchange of air through the nose can increase the risk of secretory otitis media or chronic rhinitis [2]. A consequence of the latter is the obstruction of posterior nares, and the impaired patency of the Eustachian tube pharyngeal orifice.…”

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confidence: 99%

Anatomical and Functional Assessment of Upper Airway Performance Using Innovative NasoOroSpirometer and Cone Beam Computed Tomographic Diagnostic Devices in Children with Adenoid Hypertrophy -- Observational Study

Zomkowska,

Grzybowska-Detka,

Zakrzewska

et al. 2024

Preprint

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Introduction. Adenoid hypertrophy leading to upper airway blockage is the most common cause of sleep apnoea syndrome in children. Moreover, the following disturbances can occur: normal speech resonance impairment, middle ear ventilation difficulties, and the development of an abnormal oral breathing pattern. Abnormal craniofacial growth and occlusal abnormalities can be observed as well. Objectives.The aim of the study was to confirm the relationship between respiratory disorders with the impaired upper airway patency resulting from reduced nasopharyngeal space. Moreover, an attempt was made to validate a new medical device, a NasoOroSpirometer, for diagnosing respiratory disorders resulting from adenoid hypertrophy. Design and Setting The NasoOroSpirometer is made up of three anemometric sensors (two nasal and one additional oral sensor) and is used to measure the airflow through the upper airways (UA). A measurement of the flow of air simultaneously through both nasal orifices was carried out in 105 children aged 4-8 years. The values obtained included the number of inhalations per minute, the inspiratory time and the inhalation volume. Results and discussion. The study demonstrated that children with adenoid hypertrophy had a statistically significantly lower number of inhalations through the nose than children from the control group. The current results demonstrated no statistically significant difference between the volume and the number of inhalations in both a combined analysis and in one analysis conducted separately for each nasal passage. The demonstrated statistically significant difference is most probably due to the oral compensation for the inefficient nasal respiratory pattern. A NasoOroSpirometric examination can be a screening tool in the assessment of UA patency disorders and an indicator for the eligibility for instrumental or imaging examinations.

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Ocena odsetka limfocytów CD19+CD5+ w przerosłych migdałkach gardłowych u dzieci chorych na wysiękowe zapalenie ucha środkowego

Abstract: The significant higher percentage of B lymphocytes with expressions of CD5+ receptor in hypertrophied adenoid tissue at children with otitis media with effusion are important to develop of early immunological response to inflammatory state in middle ear.

Cited by 10 publications

References 15 publications

Apoptosis-Inducing Potential of Selected Bromophenolic Flame Retardants 2,4,6-Tribromophenol and Pentabromophenol in Human Peripheral Blood Mononuclear Cells

Apoptosis-Inducing Potential of Selected Bromophenolic Flame Retardants 2,4,6-Tribromophenol and Pentabromophenol in Human Peripheral Blood Mononuclear Cells

Determination of Apoptotic Mechanism of Action of Tetrabromobisphenol A and Tetrabromobisphenol S in Human Peripheral Blood Mononuclear Cells: A Comparative Study

Anatomical and Functional Assessment of Upper Airway Performance Using Innovative NasoOroSpirometer and Cone Beam Computed Tomographic Diagnostic Devices in Children with Adenoid Hypertrophy -- Observational Study

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