Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement

Seux, Dominique; Couble, Marie‐Lise; Hartmann, Daniel; Gauthier, J.P.; Magloire, H.

doi:10.1016/0003-9969(91)90074-5

Cited by 154 publications

(132 citation statements)

References 43 publications

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“…Investigators have carried out experimental attempts in vitro to induce differentiation of pulp cells by culturing mouse dental papillae in agar microinjected with active molecules (Begue-Kirn, 1992, or more easily by obtaining long-term cultured cells from adult pulps (Tziafas et al, 1988;Bouvier et al, 1990;Veron et al, 1990;Nakashima, 1991Nakashima, , 1992Seux et al, 1991;Liang et al, 1992;Alliot-Licht et al, 1994;Nakashima et al, 1994;Shirakawa et al, 1994) grown on various substrates (collagen, fibronectin, glycosaminoglycans, calcium hydroxide etc.) or in the presence of growth factors (BMPs, TGFfs, EGF).…”

Section: Introductionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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Abstract. Pulp tissue responds to dentin injury by laying down reactionary dentin secreted by existing odontoblasts or reparative dentin elaborated by odontoblast-like cells that differentiated from precursor cells in the absence of inner dental epithelium and basement membrane. Furthermore, growth factors or active dentin matrix components are fundamental signals involved in odontoblast differentiation. In vitro, dental pulp cells cultured under various conditions are able to express typical markers of differentiation, but no culture system can re-create pulp response to dentin drilling. This paper reports the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation (BrdU-positive cells), neovascularization (positive with antitype-IV collagen antibodies), and the presence of functional (3H proline-positive) cuboidal cells close to the injured area.After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. This tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.

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Section: Introductionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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“…Such biomineralized structures in dentinal tubules around orifices suggest basic microenvironment which is beneficial in inflammation control, whilst those with lower Ca/P make acidic microenvironment inside the tubules which could provoke phase transition of CaP into unstable forms including carbonates [16,17]. Such phase transition would be attributed to decreased permeability when carbonates could interact with fibronectin in dentin matrix [18,19] …”

Section: Resultsmentioning

confidence: 99%

Evidence of Intratubular Biomineralization in a Root Canal Filled with Calcium Enriched Material over 8 Years

Y¹,

Ys²,

Yoo³

et al. 2017

Preprint

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This case report describes evidence of intratubular biomineralization in root canal filled with calcium enriched material after 8 years of clinical maintenance. The schematic findings of dentinal tubules were investigated with scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The root canal obturation material was closely adapted to root dentin surface, suggesting the possibility of chemical bonding between the two interfaces. SEM and EDS observation of dentinal tubules showed intratubular biomineralized crystal structures with Ca/P ratio in a range of 1.30-2.12, suggesting bioactive capacity of calcium enriched cement.

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“…The favorable clinical, radiographic, and histological responses obtained with calcium hydroxide are attributed to the involvement of Ca 2+ and OH -in several cellular and molecular mechanisms leading to regeneration of periapical connective tissues (9). The benefits of calcium hydroxide include anti-inflammatory action through its hygroscopic properties, such as forming calcium-proteinate bridges and inhibiting the phospholipase; the neutralization of acidic products, such as hydrolases of clasts; activation of alkaline phosphatase; and antibacterial action (16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%