Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel, Münevver; Akin, Mehriban; Geyik, Caner; Demirkol, Dilek Odaci; Ozdemir, Caglar; Bluma, Arne; Höpfner, Tim; Beutel, Sascha; Tımur, Suna; Scheper, Thomas

doi:10.1002/btpr.544

Cited by 28 publications

(17 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the results (Figure A), higher current signals were observed with SPE/PVA‐PEI/MNP compared to bare SPE, which is expected considering the presence of positively charged amino groups which attracting the redox probe. Similar data were acquired in our previous studies in which polyamidoamine (PAMAM) dendrimers were used for surface modification ,. Biomolecule immobilization (SPE/PVA‐PEI/MNP/GOx) caused a decrease in the electron transfer properties compared to the SPE/PVA‐PEI/MNP surface as shown in Figure A.…”

Section: Resultssupporting

confidence: 78%

See 1 more Smart Citation

Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One‐Use ‘Sensing in‐a‐Drop’ Applications

et al. 2018

Self Cite

View full text Add to dashboard Cite

Stabilization of biomolecules on matrices is critically important. Here we constructed composite nanofibers with magnetic features by electro‐spinning of magnetic nanoparticles (MNP) in an appropriate polymer matrix on a collector surface for multiple uses. ‘Poly(vinylalcohol)‐polyethyleneimine/Fe3O4 magnetic nanoparticles’ (PVA‐PEI/MNP) composite nanofiber was used on the screen printed electrodes (SPE) to serve as a platform for the glucose oxidase (GOx) biofilm as the model biomolecule. To show the potential application of this material as a biosensor component, the resulted biofilm, called ‘PVA‐PEI/MNP/GOx’, was fixed on SPE via a neodymium magnet for the electrochemical detection of glucose at −0.7 V where oxygen consumption due to enzymatic reaction was measured. The main advantage of the obtained magnetic biomembrane is that it allows analysis with a single drop. After step‐by‐step surface modifications, analytical characterization was performed using various techniques, such as voltammetry, electrochemical impedance spectroscopy, Fourier‐transform infrared spectroscopy and X‐Ray photoelectron spectroscopy as well as microscopic techniques. The linear range of the PVA‐PEI/MNP/GOx surface was found as 0.0125 to 0.5 mM with a limit of detection of 11.5 μM for glucose as analyte. The application of PVA‐PEI/MNP/GOx for glucose detection in synthetic samples was carried out. Data proved the potential use of the ‘PVA‐PEI/MNP’ surface as a biomolecule immobilization platform for disposable biosensor applications.

show abstract

Section: Resultssupporting

confidence: 78%

“…Furthermore, a wider linearity was observed when compared to a previous work in which an impedimetric detection mode was utilized . Previously, our group reported a biosensor constructed by PAMAM dendrimers, which it exhibited a better linear range but higher LOD for glucose compared with PVA‐PEI/MNP/GOx.…”

Section: Resultsmentioning

confidence: 84%

Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One‐Use ‘Sensing in‐a‐Drop’ Applications

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…As can be seen from the Table 1, the K m is much lower than those reported for Copolymers of vinyl alcohol with thiophene side groups and pyrrole/GOx, 47 Poly(2-Dodecyl-4,7-di(thiophen-2-yl)-2H-benzo[d] [1,2,3]triazole)/GOx, 31 Poly (N-(4-(3-thienylmethylene)-oxycarboyl phenyl) maleimideco-pyrrole/GOx. 41 The integration of biosensors to FIA mode has a large variety of applications (37,38). The advantage of FIA devices is their possible uses in multiple measurements simultaneously (39).…”

Section: Analytical Characteristicsmentioning

confidence: 99%

Oligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applications

Seven

Demirdoven

Yıldırım

et al. 2013

Journal of Macromolecular Science, Part A

Self Cite

View full text Add to dashboard Cite

Thiosemicarbazone (TSC) matrices are constructed by electrochemically oligomerization on the graphite electrodes to investigate their matrix properties in terms of biomolecule immobilization for the biosensor applications. Amino functionalized oligomeric TSCs were successfully applied for the glucose oxidase (GOx) immobilization using glutaraldehyde (GA) as the crosslinker. Scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FT-IR) measurements are used to characterize the modified surface. Electrochemical measurements of the GOx biosensor were performed at ambient conditions at −0.7 vs. Ag/AgCl electrode by following the oxygen consumption resulting from the enzyme activity, in the presence of glucose substrate. Linearity, operational stabilities and repeatability are investigated in detail, as well as optimization of pH and enzyme amount,. The optimized biosensor shows a very good linearity between 0.05 mM and 1.0 mM glucose with a 10 s response time. Kinetic parameters such as K m and I max are also calculated. Besides the batch configuration, the analytical characteristics of the system were also evaluated in flow injection analysis (FIA) mode. Finally, the new biosensor configuration was applied for glucose determination on a real sample.

show abstract

“…Literature, relating to different applications of biosensors in process monitoring is listed in Table .…”

Section: Bioprocess Monitoring Systemsmentioning

confidence: 99%

Sensor systems for bioprocess monitoring

Biechele

Busse

Solle

et al. 2015

Engineering in Life Sciences

Self Cite

173

158

View full text Add to dashboard Cite

The ability to measure all process variables is of great importance in the field of bioprocess monitoring and control, and continuous, real‐time measurements are highly desired. The more complete and real‐time the measurements are, the more stable, reproducible, and efficient the process can be, leading to reproducibly high‐quality products. This additional information allows the operator to better document the entire process. The process analytical technologies initiative of the US Food and Drug Administration is strongly related to the analysis and control of biopharmaceutical processes. The aim of the initiative is to create processes, generating products of ensured quality by measuring quality‐related process variables. The quality of the product is enhanced by a deep understanding of the process, which is enabled by an effective and suitable sensor system. The aim of this review is to provide an overview of current and emerging sensors for bioprocess monitoring. Sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, are discussed, as well as sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition. Furthermore, sensor systems are discussed. These involve sensors (especially biosensors) that are not implemented directly inside the bioreactor but rather are used in conjunction with sample‐taking systems such as flow injection analysis. A major focus is given to spectroscopic sensors, which are noninvasive and offer interesting options for a simultaneous analysis of various compounds. Since data handling is extremely important for this kind of sensor, chemometrics are also included. Soft sensors are discussed as technology that allows a user to incorporate more process data as it become available. Finally, the current state of disposable sensor technology is presented. These sensors are needed for the growing area of disposable and continuous biomanufacturing.

show abstract

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Cited by 28 publications

References 53 publications

Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One‐Use ‘Sensing in‐a‐Drop’ Applications

Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One‐Use ‘Sensing in‐a‐Drop’ Applications

Oligomeric Thiosemicarbazones as Novel Immobilization Matrix in Biosensing Applications

Sensor systems for bioprocess monitoring

Contact Info

Product

Resources

About