Offset of the vacuolar potential of Characean cells in response to electromagnetic radiation over the range 250 Hz‐250 kHz

Montaigne, Kathleen Marie; Pickard, William F.

doi:10.1002/bem.2250050104

Cited by 14 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exogenous field modulation of cartilage and chondrocyte biosynthesis has been re-In this study, we first addressed the question of what range of field magnitudes and frequencies could alter protein synthesis in cartilage explants. These field magnitudes and frequencies may suggest possible mechanisms by which fields can interact with cells (17,21,34). Secondly, we compared the effects of electric fields with the stress response (8,30) induced by heating or other mechanisms.…”

Section: A Maccinitie E T a Lmentioning

confidence: 99%

“…and lo4 Hz. This range of field magnitudes and frequencies was chosen on the basis of (a) the fields induced in cartilage by physiological mechanical loading and (b) the field magnitudes and frequencies that were estimated, on the basis of theoretical models (frequencies of less than 100 kHz and current densities near l mA/cm2), to be capable of producing physiological changes (17,21,25). Incorporation of 35S-methionine was used as a marker for protein synthesis.…”

Section: A Maccinitie E T a Lmentioning

confidence: 99%

See 1 more Smart Citation

Electric field stimulation can increase protein synthesis in articular cartilage explants

MacGinitie

Gluzband

Grodzinsky

1994

Journal Orthopaedic Research

View full text Add to dashboard Cite

It has been hypothesized that the electric fields associated with the dynamic loading of cartilage may affect its growth, remodeling, and biosynthesis. While the application of exogenous fields has been shown to modulate cartilage biosynthesis, it is not known what range of field magnitudes and frequencies can alter biosynthesis and how they relate to the magnitudes and frequencies of endogenous fields. Such information is necessary to understand and identify mechanisms by which fields may act on cartilage metabolism. In this study, incorporation of 35S-methionine was used as a marker for electric field-induced changes in chondrocyte protein synthesis in disks of cartilage from the femoropatellar groove of 1 to 2-week-old calves. The cartilage was stimulated sinusoidally at 1, 10, 100, 10(3), and 10(4) Hz with current densities of 10-30 mA/cm2. Incorporation was assessed in control disks maintained in the absence of applied current at 37, 41, and 43 degrees C. The possibility that applied currents would induce synthesis of the same stress proteins that are caused by heating or other mechanisms was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and examination of gel fluorographs. Total radiolabel incorporation in cartilage that had been stimulated relative to incorporation in the controls increased with current density magnitudes greater than 10 mA/cm2. The increase was greatest at 100 Hz and 1 kHz, and it depended on the position on the joint surface from which the cartilage samples were taken. Together, these results suggest that endogenous electric fields could affect cartilage biosynthesis. Stress proteins were not induced at any current density when the electrodes were electrically connected but chemically isolated from the media by agarose bridges. Stress proteins were observed for disks incubated at temperatures greater than 39 degrees C (no field) and when the stimulating platinum electrodes were in direct contact with the media bathing the cartilage disks. Therefore, the increase in incorporation of 35S-methionine due to applied fields with the use of chemically isolated electrodes did not appear to be associated with stress response.

show abstract

Section: A Maccinitie E T a Lmentioning

confidence: 99%

Section: A Maccinitie E T a Lmentioning

confidence: 99%

Electric field stimulation can increase protein synthesis in articular cartilage explants

MacGinitie

Gluzband

Grodzinsky

1994

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…The nonlinearity was demonstrated by the observation that membranes rectified RF signals of average field $1 V/m frequencies below a few kHz. However, the size of the rectified signal fell rapidly at higher frequencies and at 100 kHz, was a hundred times less than the low frequency value [Montaigne and Pickard, 1984]. At lower frequencies, the field across the membrane is larger than the average field by several thousand, but as discussed earlier, this enlargement disappears at higher frequencies.…”

Section: Low Frequency Electric Fields From Demodulationmentioning

confidence: 74%

Mechanisms for interaction between RF fields and biological tissue

Challis

2005

Bioelectromagnetics

191

127

View full text Add to dashboard Cite

Interaction of radiofrequency (RF) fields with biological tissue can involve either electric or magnetic fields. Many interaction mechanisms have been considered, both thermal and nonthermal, but it has not been established that any of these could result in adverse health effects at radiation levels below guidelines. The principles underlying most of these mechanisms have been well reviewed. The aim of the present study is to give a qualitative discussion of some of the more recently published work.

show abstract

“…Other possible biological effects are associated with cell membranes and the movement of currents through the membrane in either direction. Membranes are known to have strongly non-linear electric properties (Montaigne and Pickard, 1984). When a voltage is applied across the membrane, the current that flows is not always proportional to the voltage.…”

Section: Abbreviations Listmentioning

confidence: 99%

Effect of Whole-Body Exposure to High-Frequency Electromagnetic Field on the Brain Cortical and Hippocampal Activity in Mouse Experimental Model

Barcal¹,

Vožeh²

2007

Neuroquantology

View full text Add to dashboard Cite

Evaluation of the direct registration of brain cortical and hippocampal activity during a high-frequency electromagnetic field (HF-EMF) exposure was performed. Experimental procedures were done under general anesthesia (urethane, 20%, 2g/kg i.p.) in Lurcher mutant mice, wild type (healthy littermates) were used as controls. Animals were exposed to the HF-EMF with frequency corresponding to cellular phones (900 MHz). We used of gel electrodes (silicon tubes or glass microcapillary filled with agar) where the connection with classical electrodes was located out of HF-EMF space. ECoG evaluation showed a distinct shift to lower frequency components but clear effect has been observed only in wild type (healthy) mice whereas in Lurcher mutant mice only gentle differences between frequency spectra were found. Measurement of hippocampal rhythmicity showed gentle changes with increase of higher frequencies (i.e. opposite effect than in cortex) and changes in theta oscillations registered from a dentate gyrus and CA1 area in both types of animals (healthy and mutant). These findings support an idea about possible influencing the central nervous system by HF-EMF exposure and support also some recent results about possible health risks resulting from cellular phones use.

show abstract

Offset of the vacuolar potential of Characean cells in response to electromagnetic radiation over the range 250 Hz‐250 kHz

Cited by 14 publications

References 11 publications

Electric field stimulation can increase protein synthesis in articular cartilage explants

Electric field stimulation can increase protein synthesis in articular cartilage explants

Mechanisms for interaction between RF fields and biological tissue

Effect of Whole-Body Exposure to High-Frequency Electromagnetic Field on the Brain Cortical and Hippocampal Activity in Mouse Experimental Model

Contact Info

Product

Resources

About