Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources

Abstract

Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1α (EF-1α) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322T = CBS 12778T = NRRL Y-63657T), Ogataea histrianica sp. nov. (type strain: ZIM 2463T = CBS 12779T = NRRL Y-63658T) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896T = CBS 12735T = NRRL Y-63656T).

Early Ongoing Speciation of Ogataea uvarum Sp. Nov. Within the Grape Ecosystem Revealed by the Internal Variability Among the rDNA Operon Repeats

“…The closest relatives were Ogataea philodendri (16 substitutions equivalent to 1.45% difference), O. polymorpha (48 substitutions equivalent to 4.36% difference), and O. angusta (52 substitutions equivalent to 4.69% difference). Members of the clade rather distant to the new species were O. kodamae , known as a species associated with insects (83 substitutions equivalent to 7.55% difference) ( Mikata and Yamada, 1995 ), O. naganishii , isolated from plant exudates and rotted logs (94 substitutions equivalent to 8.55% difference) ( Kurtzman and Robnett, 2010 ; Kurtzman et al, 2011 ), Candida pignaliae , usually associated with plants (82 substitutions equivalent to 7.56% difference) ( Péter et al, 2010 ), O. histrianica (89 substitutions equivalent to 8.08% difference), O. kolombanensis (92 substitutions equivalent to 8.40% difference), and O. deakii (108 substitutions equivalent to 9.86% difference), isolated from olive oil and rotten wood ( Cadez et al, 2013 ). The assimilation profile of the proposed species differs for several traits from the closest species of the clade ( Table 1 ).…”

“…The first description of the genus Ogataea was proposed on the basis of the type species Ogataea minuta ( Yamada et al, 1994 ) that showed the assimilation of the potassium nitrate and the presence of asci containing one to four ascospores of pileiform shape. To date, more than 40 species belonging to the genus Ogataea have already been described ( Péter et al, 2007 , 2008 ; Ji and Bai, 2008 ; Limtong et al, 2008 ; Cadez et al, 2013 ).…”

Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

“…Yeast species of the Ogataea clade share the ability to grow with methanol as the sole carbon source (Kurtzman, 2011; Čadež et al , 2013). In this clade, only O. salicorniae does not assimilate methanol (Kurtzman, 2011).…”

“…In this clade, only O. salicorniae does not assimilate methanol (Kurtzman, 2011). In addition to the 31 known species of Ogataea listed by Kurtzman (2011), nine species belonging to this clade were described recently (Čadež et al , 2013). Most species of Ogataea are associated with rotting wood, the external surfaces of leaves or insect frass, or as minor components of yeast communities on bodies of water and olive oil (Kurtzman 2011; Koowadjanakul et al , 2011; Limtong et al , 2013; Čadež et al , 2013).…”

“…Several species of the Ogataea clade have been described as associated with the phylloplane, such as O . allantospora , O. kanchanaburienis , O. nitratoaversa , O. phyllophila , O. saltuana and O. wangdongensis (Koowadjanakul et al ., 2011; Péter et al , 2011; Čadež et al , 2013; Limtong et al , 2013). It is likely that these methylotrophic yeasts utilize methanol and other leaching carbon sources from the leaves, as suggested by Péter et al (2007).…”