Oligomeric proanthocyanidins from Rumex acetosa L. inhibit the attachment of herpes simplex virus type-1

Gescher, Kirsten; Hensel, Andreas; Hafezi, Wali; Derksen, Andrea; Kühn, Joachim

doi:10.1016/j.antiviral.2010.10.007

Cited by 101 publications

(89 citation statements)

References 27 publications

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“…Antiherpetic activity has been shown for aqueous plant extracts from Echinacea pallidum [32], Rumex acetosa [33] and Rhododendron ferrugineum [34], for polysaccharides derived from marine algae [35], for steroidal compounds against adenovirus [36] and an ethylacetate-soluble fraction from the lichen Ramalina farinacea against HIV [37]. Although all analyzed drugs in our study demonstrated virucidal activity, Melissa extract demonstrated a higher virucidal activity when compared to single compounds.…”

Section: Discussionmentioning

confidence: 65%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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Background: Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs. Methods: An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-coumaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Results: When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 µg/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosmarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents. Conclusion: Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro.

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Section: Discussionmentioning

confidence: 65%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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“…NSC 143101, in particular, is structurally similar to proanthocyanidins (oligomeric proanthocyanidins or condensed tannins), a subgroup of the flavonoid class of polyphenols. Relevant to viral entry, proanthocyanidins have been shown to inhibit entry of herpes simplex virus (25,26) and HIV-1 (22,54). Although no published studies have evaluated the antiviral effect of proanthocyanidins on HCV entry, numerous studies have demonstrated that some flavonoids can inhibit HCV replication and HCVpp entry (3,44,68,74).…”

Section: Discussionmentioning

confidence: 99%

Identification of Hepatitis C Virus Inhibitors Targeting Different Aspects of Infection Using a Cell-Based Assay

Sáinz

Petukhov

et al. 2012

Antimicrob Agents Chemother

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With 2 to 3% of the worldwide population chronically infected, hepatitis C virus (HCV) infection continues to be a major health care burden. Unfortunately, current interferon-based treatment options are not effective in all patients and are associated with significant side effects. Consequently, there is an ongoing need to identify and develop new anti-HCV therapies. Toward this goal, we previously developed a cell-based HCV infection assay for antiviral compound screening based on a low-multiplicity-of-infection approach that uniquely allows for the identification of antiviral compounds that target cell culture-derived HCV (HCVcc) at any step of the viral infection cycle. Using this assay, here we report the screening of the NCI Diversity Set II library, containing 1,974 synthesized chemical compounds, and the identification of compounds with specific anti-HCV activity. In combination with toxicity counterscreening, we identified 30 hits from the compound library, 13 of which showed reproducible and dose-dependent inhibition of HCV with mean therapeutic indices (50% cytotoxic concentration [CC 50 ]/50% effective concentration [EC 50 ]) of greater than 6. Using HCV pseudotype and replicon systems of multiple HCV genotypes, as well as infectious HCVcc-based assembly and secretion analysis, we determined that different compounds within this group of candidate inhibitors target different steps of viral infection. The compounds identified not only will serve as biological probes to study and further dissect the biology of viral infection but also should facilitate the development of new anti-HCV therapeutic treatments. Hepatitis C virus (HCV) is a hepatotropic enveloped positivestrand RNA virus (family Flaviviridae) that infects the parenchymal cells of the liver (i.e., hepatocytes) (69, 70). HCV infection begins with interaction of the viral particle with the target cell via a relatively undefined multistep process of binding between the virion and multiple cell surface receptors, which include the cluster of differentiation 81 (CD81) tetraspanin protein (7,34,56,80,84), the scavenger receptor class B member I (SR-BI) (also known as SCARB1) (7,30,38,63,83), and the tight-junction proteins claudin-1 (CLDN1) (20) and occludin (OCLN) (8,47,57). In addition to these four host factors, we more recently showed that the cellular Niemann-Pick C1-like 1 (NPC1L1) cholesterol uptake receptor also functions as an HCV entry factor via a virion particle cholesterol-associated mechanism (60), and Lupberger et al. have recently shown that the host cellular epidermal growth factor receptor (EGFR) and ephrin receptor A2 (EphA2) function as cofactors during viral cell entry (49).Following entry, the viral genome (ϳ9.6 kb) is translated by an internal ribosome entry site (IRES)-dependent process into a single viral polyprotein that is subsequently co-and posttranslationally cleaved into structural (core, E1, E2, and p7) and nonstructural (NS2, NS3, NS4A, NS4B, NS5A, and NS5B) proteins by host and viral proteases (29,46). The NS prote...

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“…These plants were also used to treat diarrhea (U. fulva or U. rubra; R. acetosella), skin inflammations (U. fulva or U. rubra; R. acetosella; R. palmatum), viral infections, respiratory diseases (R. acetosella), intestinal bleeding, pancreatitis, kidney diseases (R. palmatum), convalescence, cough, and sore throat (U. fulva or U. rubra) (Castleman, 1991;Bradley, 1992;Duke, 1997;Gescher, Hensel, Hafezi, Derksen, & Kühn, 2011). A. lappa is used as diuretic, depurative, digestive stimulant, and anti-allergic (Soh et al, 2011;De Almeida et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Anti-leukemic activity of a four-plant mixture in a leukemic rat model

Kabeel

Ghoneim

Mansy

2018

JoBAZ

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Oligomeric proanthocyanidins from Rumex acetosa L. inhibit the attachment of herpes simplex virus type-1

Cited by 101 publications

References 27 publications

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Identification of Hepatitis C Virus Inhibitors Targeting Different Aspects of Infection Using a Cell-Based Assay

Anti-leukemic activity of a four-plant mixture in a leukemic rat model

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