On-Line Measurement of Glucose in a Rotating Wall Perfused Vessel Bioreactor Using an Amperometric Glucose Sensor

Abstract

A high performance, long-term, stable glucose sensor was developed and evaluated for continuous measurement of glucose for use in National Aeronautics and Space Administration space bioreactors while using a rotating wall perfused vessel system to support a baby hamster kidney (BHK-21) cell culture. The sensor employs a glucose oxidase multilayered membrane with a hydrogen peroxide electrode system. The output of the sensor was converted to glucose concentration utilizing the two-point calibration method. The sensor, tested over four 30 day bioreactor cell runs, showed a steady-state response time of less than 15 min. The sensor was able to measure glucose continuously and accurately for 16 days while using a single calibration and 30 days using two sets of calibrations, resulting in a standard error of prediction of ±0.5 normalmM as against a benchtop Beckman glucose analyzer. Evaluation conditions like cell culture pH, incubator temperature conditions, and operational flow rate, were found to have a very little effect on the sensor performance. © 2002 The Electrochemical Society. All rights reserved.

Continuous glucose monitoring and control in a rotating wall perfused bioreactor

“…The calibration curve for the glucose sensor was found to be linear in the concentration range between 0 and 17.2 mM (Xu et al, 2002). The steady-state response time was measured to be <5 min, which is adequate for bioreactor control and monitoring.…”

“…We previously reported the successful development of a reliable, stable, sterilizable amperometric glucose sensor for in-line continuous monitoring of glucose in a perfused bioreactor in which mammalian cells were cultured (Xu et al, 2002). We have chosen an in-line measurement, coupled to a simple control mechanism for regulating glucose concentration in cell culture.…”

On‐line monitoring of human prostate cancer cells in a perfusion rotating wall vessel by near‐infrared spectroscopy

“…In addition, utilization of RWVs during spaceflight missions necessitates long-term stable sensor systems with minimal maintenance requirements. Previously described on-line monitoring systems for RWVs include pH monitoring and glucose monitoring (Xu et al, 2002). Using the amperometric glucose sensor described by Xu et al (2002), it was possible to monitor glucose continuously for 16 days while using a single calibration and for 30 days using two sets of calibrations.…”

“…Previously described on-line monitoring systems for RWVs include pH monitoring and glucose monitoring (Xu et al, 2002). Using the amperometric glucose sensor described by Xu et al (2002), it was possible to monitor glucose continuously for 16 days while using a single calibration and for 30 days using two sets of calibrations. Their reported standard error of prediction (SEP) was 0.5 mM (Xu et al, 2002).…”

“…The advantage of spectroscopic sensors is that multiple components can be measured simultaneously and noninvasively (Rhiel et al, 2002a). Simultaneous measurement eliminates the need for multiple sensors, and noninvasive measurement avoids the need for elaborate sterilizations, such as, for example, with the amperometric sensor described by Xu et al (2002). Rhiel et al (2002a) generated calibration models able to monitor glucose, lactate, glutamine, and ammonia with SEPs of 0.82, 0.94, 0.55, and 0.76 mM, respectively (Rhiel et al, 2002a).…”