On-line monitoring of UV-induced lipid peroxidation products from human skin in vivo using proton-transfer reaction mass spectrometry

Steeghs, M.M.L.; Moeskops, B.W.M.; Swam, Karen van; Cristescu, Simona M.; Scheepers, Paul T.J.; Harren, F.J.M.

doi:10.1016/j.ijms.2006.02.015

Cited by 46 publications

(40 citation statements)

References 30 publications

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“…A major advantage of using H 3 O + as the primary reactant ion is that the proton affinity of the normal constituents of air occurring in high concentration, like NO, O 2 , CO, CO 2 , N 2 , is lower that of H 2 O molecules. These compounds do not interfere with the measurement because they do not react with H 3 O + ions (Ípan!l and Smith, 2001;Steeghs et al, 2006). Figure 8 shows the concentration-times series for ionic mass m/z = 69 (= m 1 + m H , tentatively identified as isoprene with molecular mass m 1 = 68 u) during one night (Amann et al, 2004, in comparison with the heart rate.…”

Section: Analysis and Detectionmentioning

confidence: 99%

“…The main advantage of proton transfer chemical ionization is that in most cases it does not induce any significant fragmentation, and when it does only few fragments are produced. Soft-ionization results in only one or two significant characteristic ions, the matrix of signals is much less complicated than with other mass spectrometry techniques (Ípan!l and Smith, 2001;Steeghs et al, 2006;Amann et al, 2007).…”

Section: Analysis and Detectionmentioning

confidence: 99%

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Human exhaled air analytics: biomarkers of diseases

Buszewski

Kęsy

Ligor

et al. 2007

Biomedical Chromatography

688

531

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Over the last few years, breath analysis for the routine monitoring of metabolic disorders has attracted a considerable amount of scientific interest, especially since breath sampling is a non-invasive technique, totally painless and agreeable to patients. The investigation of human breath samples with various analytical methods has shown a correlation between the concentration patterns of volatile organic compounds (VOCs) and the occurrence of certain diseases. It has been demonstrated that modern analytical instruments allow the determination of many compounds found in human breath both in normal and anomalous concentrations. The composition of exhaled breath in patients with, for example, lung cancer, inflammatory lung disease, hepatic or renal dysfunction and diabetes contains valuable information. Furthermore, the detection and quantification of oxidative stress, and its monitoring during surgery based on composition of exhaled breath, have made considerable progress. This paper gives an overview of the analytical techniques used for sample collection, preconcentration and analysis of human breath composition. The diagnostic potential of different disease-marking substances in human breath for a selection of diseases and the clinical applications of breath analysis are discussed.

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Section: Analysis and Detectionmentioning

confidence: 99%

Section: Analysis and Detectionmentioning

confidence: 99%

Human exhaled air analytics: biomarkers of diseases

Buszewski

Kęsy

Ligor

et al. 2007

Biomedical Chromatography

688

531

View full text Add to dashboard Cite

show abstract

“…In addition, S. lycopersicum 'Moneymaker' and three wild tomatoes (LA2560, PI127826, and GI1560) were analyzed for the production of green leaf volatiles (GLVs) by proton transfer reaction-mass spectrometry (Steeghs et al, 2006). Significant amounts of GLVs could only be detected after wounding the tomato plants (data not shown), ruling out the involvement of GLVs in B. tabaci host location.…”

Section: Determining Headspace Components As Cues For Repellencementioning

confidence: 99%

The Role of Specific Tomato Volatiles in Tomato-Whitefly Interaction

Bleeker

Diergaarde²,

Ament³

et al. 2009

Plant Physiology

260

228

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Bemisia tabaci (whitefly) infestations and the subsequent transfer of viruses are the cause of severe losses in crop production and horticultural practice. To improve biological control of B. tabaci, we investigated repellent properties of plant-produced semiochemicals. The mix of headspace volatiles, collected from naturally repellent wild tomato accessions, influenced B. tabaci initial choice behavior, indicating a role for plant semiochemicals in locating host plants. A collection of wild tomato accessions and introgression lines (Solanum pennellii LA716 3 Solanum lycopersicum 'Moneyberg') were extensively screened for attractiveness to B. tabaci, and their headspace profiles were determined by means of gas chromatography-mass spectrometry. Correlation analysis revealed that several terpenoids were putatively involved in tomato-whitefly interactions. Several of these candidate compounds conferred repellence to otherwise attractive tomato plants when applied to the plant's branches on paper cards. The sesquiterpenes zingiberene and curcumene and the monoterpenes p-cymene, a-terpinene, and a-phellandrene had the strongest effects in free-choice bioassays. These terpenes also elicited a response of receptors on the insect's antennae as determined by electroantennography. Conversely, the monoterpene b-myrcene showed no activity in both assays. B. tabaci apparently uses, besides visual cues, specific plant volatile cues for the initial selection of a host. Altering whitefly choice behavior by manipulation of the terpenoid composition of the host headspace may therefore be feasible.

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“…Sealing a participant's limb into a ventilated Tedlar bag and analysing the exhaust by selected ion flow tube mass spectrometry (SIFT-MS) provided targeted on-line acetone [17] and ammonia levels [18]. A proton transfer reaction mass spectrometry (PTR-MS) technique demonstrated how to sample synthetic-air directed over the skin's surface [19]. The benefits of these on-line approaches to the researcher are speed, selectivity and sensitivity, and for time-resolved targeted analysis they represent the current state-of-the-art.…”

Section: Sampling Voc-profiles In Skinmentioning

confidence: 99%

Volatile organic compound markers of psychological stress in skin: a pilot study

Martin¹,

Turner²,

Bandelow³

et al. 2016

J. Breath Res.

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The forehead was studied as a possible sampling site for capturing changes in volatile organic compound (VOC) profiles associated with psychological-stress. Skin-VOCs were sampled with a polydimethylsilicone (PDMS)-coupon and the resulting VOCs were recovered and analysed with two-stage thermal desorption gas chromatography-mass spectrometry.15 young adult volunteers (19 yr to 26 yr) participated in two interventions run in a randomised crossover design. One intervention, termed "Neutral", required the participants to listen to peaceful music, the other, termed a "paced audio serial addition task", required the participants to undertake a series of rapid mental arithmetic calculations in a challenging environment that induced a stress response. Skin-VOC samples were taken during each intervention. The resultant data were processed with dynamic background compensation, deconvolved, and registered to a common retention index scale.The importance of freezing skin patch samplers to -80 o C was determined during the method development phase of this study. The cumulative distribution function of the GC-MS data indicates the possibility that PDMS-coupons are selective towards the lower volatility VOC components in skin. The frequency distribution of the GC-MS data was observed to be approximately log-normal, and on the basis of this study, a further twoorders of magnitude reduction in sensitivity may be required before the complete skin-VOC profile may be characterised.Multi-variate analysis involving Pareto-scaling prior to partial least squares discriminant analysis identified four VOCs with the highest probability of contributing to the variance between the two states, and the responses to these VOCs were modelled with principle components analysis (PCA). Two VOCs, benzoic acid and n-decanoic acid were upregulated (14-and 8-fold respectively) and appear to be PASAT sensitive, with areas under (AUC) their receiver operator characteristic (ROC) curves of 0.813 and 0.852 respectively. A xylene isomer and 3-carene were down regulated 75 % and 97% respectively, and found to be predictive of the neutral intervention (ROC AUC values of 0.898 and 0.929 respectively). VOC profiles in skin appear to change with stress either due to increased elimination, elevated bacterial activity, or perhaps increased oxidative pathways.2

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On-line monitoring of UV-induced lipid peroxidation products from human skin in vivo using proton-transfer reaction mass spectrometry

Cited by 46 publications

References 30 publications

Human exhaled air analytics: biomarkers of diseases

Human exhaled air analytics: biomarkers of diseases

The Role of Specific Tomato Volatiles in Tomato-Whitefly Interaction

Volatile organic compound markers of psychological stress in skin: a pilot study

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