On‐line supercritical fluid extraction/chromatography system for trace analysis of pesticides in soybean oil and rendered fats

Abstract

Abstract.A method for trace analysis incorporating solid phase extraction (extraction and clean-up) with supercritical fluid chromatography (SFC) for analytes in fats and oils has been developed. Direct coupling (on-line) of supercritical fluid solid phase extraction with supercritical fluid chromatography (SFSPEISFC) allows analysis of solutes in fats and oils for which on-line SFElSFC is unsuitable. The main feature of SFSPEISFC is that extraction and clean-up are accomplished in one step using unmodified supercritical carbon dioxide. Extraction/clean-up is monitored by a photodiode array detector which allows fractionation. The operational set-up and potential usefulness of the SFSPE/SFC system and applications for pesticide analysis of lipid sample matrices are described.

Supercritical Fluid Chromatography and Extraction

“…Murugaverl and Voorhees combined the trace analysis capabilities of on-line SFE/SFC with on-line solid-phase extraction cleanup to determine pesticides in fats and oils (197). This arrangement greatly reduced sample handling.…”

Integrated SFE/SFC/MS System for the Analysis of Pesticides in Animal Tissues

“…The unique properties of supercritical fluids make them attractive media for extraction and chromatography of compounds such as the carbamate pesticides. Supercritical fluid extraction (SFE) and chromatography of pesticides with pure or modified supercritical CO 2 from a variety of sample matrices have been reported in the literature (Wheeler and McNally, 1989;France et al, 1991;Hopper and King, 1991;Murugaverl and Voorhees, 1991;Nam et al, 1991;Howard and Taylor, 1992;Nishikawa, 1992a,b;Snyder et al, 1992;Howard et al, 1993;Lopez-Avila et al, 1993;Rochette et al, 1993;Wigfield and Lanouette, 1993;Berger et al, 1994;Nam and King, 1994;Robertson and Lester, 1994;Argauer et al, 1995;Lehotay, 1997).…”

Supercritical fluid extraction strategies of liquid-based matrices

“…Murugaverl and Voorhees [56] have developed an SFE-cSFC procedure for the detection of pesticides in soybean oil. The vegetable oil was first mixed with C I8 sorbent and then loaded onto the top of an extraction column which had been dry packed (tap and fill method) with various sorbents, including silica, C 18 , C 2 , cyanopropyl and aminopropyl.…”