On the Benefit of Pure Glycyrrhizic Acid on the Function and Metabolic Activity of Isolated Pancreatic Langerhans Islets in vitro

Rahimifard, Mahban; Navaii-Nig, Mona; Nilli-Ahma, Amir; Pourkhalil, Nazila; Baeeri, Maryam; Mohammadir, Azadeh; Abdollahi, Mohammad

doi:10.3923/ajava.2012.1212.1218

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Oxidative stress is the end result of an imbalance among antioxidants and free radicals which are involved in cellular impairment. 15 For instance, cumulative ROS in pancreatic β-cells decreases the insulin gene expression and insulin release and also injures the islets. 16 Since the β-cells have little level of antioxidant protection enzymes such as superoxide dismutase (SOD) and catalase (CAT), these cells enormously have excessive sensitivity to free radical-induced impairment.…”

Section: Introductionmentioning

confidence: 99%

Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin

et al. 2018

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The growing use of carbon nanotubes (CNTs) emphasizes the importance of its potential toxic effects on the human health. Previous studies proved that CNTs caused oxidative stress and decreased cell viability. On the other hand, reactive oxygen species (ROS) and oxidative stress impaired β-cell functions and reduced the insulin secretion. However, there is not any study on the effects of CNTs on islets and β-cells. Therefore, the present study aimed to evaluate the effects of single-walled CNTs (SWCNTs) on oxidative stress in islets in addition to the protective effects of naringin (NRG) as an antioxidant . We examined the effects of SWCNTs and naringin on islets by 3,4 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay; measurement of insulin secretion, ROS, and malondialdehyde (MDA); activities of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) peroxidase (GSH-Px); and content of GSH and mitochondrial membrane potential (MMP). The MTT assay demonstrated that decreased viability of islets cells was dose-dependent with exposure to SWCNTs. Further studies revealed that SWCNTs decreased insulin secretion and MMP, induced the formation of ROS, increased the level of MDA, and decreased the activities of SOD, GSH-Px, and CAT and content of GSH. Furthermore, the pretreatment of islets with naringin significantly reverted back these changes. These findings revealed that SWCNTs might induce the oxidative stress to pancreatic islets, causing the occurrence of diabetes, and the protective effects of naringin that was mediated by augmentation of the antioxidant defense system of islets. Our research indicated the necessity for further in vivo and in vitro researches on the effects of SWCNTs and naringin on diabetes.

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Section: Introductionmentioning

confidence: 99%

Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin

et al. 2018

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Zinc Oxide Nanoparticles Reduce Apoptosis and Oxidative Stress Values in Isolated Rat Pancreatic Islets

Shoae-Hagh

Rahimifard

Navaei-Nigjeh

et al. 2014

Biol Trace Elem Res

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Although toxic effects of zinc oxide nanoparticles (ZnO NPs) have been previously studied, there are still controversies in terms of dose, size, shape, and affecting cells. By such a perspective, in this study, small size of ZnO NPs with a diameter of 10 nm at low concentrations was studied for any effect on the viability and function of isolated rat pancreatic islets. Islets of Langerhans were isolated and assessed for viability, functionality (insulin secretion), cytosolic reactive oxygen species (ROS), and apoptosis by flow cytometry. The LC50 of ZnO NPs was found at 1,400 ng/mL at the first phase of the study. A meaningful increase in viability of islets and insulin secretion in basal and even stimulated concentrations of glucose was found by ZnO NPs (70 ng/mL) with p < 0.001 and p < 0.05, respectively. Likewise, ZnO NPs in 70 ng/mL concentration decreased cytosolic ROS generation (p < 0.05). In the meantime, the percentage of early stage of apoptotic cells dropped down to 17 % (from 29 % of control). These results for the first time confirm that ZnO NPs are not only safe when used at dose of 70 ng/mL but also improve viability and function of pancreatic islets and meanwhile reduce oxidative stress and prevent cells from entering the apoptotic phase.

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New Formulations from Acacia Nilotica L. and Glycyrrhiza Glabra L. for Oral Ulcer Remedy

Mostafa¹,

Ammar²,

El-Anssary³

2013

MJIWAS

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On the Benefit of Pure Glycyrrhizic Acid on the Function and Metabolic Activity of Isolated Pancreatic Langerhans Islets in vitro

Cited by 4 publications

References 8 publications

Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin

Mice pancreatic islets protection from oxidative stress induced by single-walled carbon nanotubes through naringin

Zinc Oxide Nanoparticles Reduce Apoptosis and Oxidative Stress Values in Isolated Rat Pancreatic Islets

New Formulations from Acacia Nilotica L. and Glycyrrhiza Glabra L. for Oral Ulcer Remedy

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