On the composition of capillary and venous blood serum

Kupke, Ingeborg R.; Kather, B.; Zeugner, S.

doi:10.1016/0009-8981(81)90376-4

Cited by 69 publications

(49 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lower Hb concentration in capillary blood could be due to biological factors. Kupke et al demonstrated that total protein concentration in capillary serum is~3.3% lower than venous serum in fasting healthy adults [49]. The small size of capillary blood vessels and the lower red cell volume in capillary blood [50] may have contributed to the lower Hb concentrations detected by the HemoCue® device (capillary blood) in our study.…”

Section: Discussionmentioning

confidence: 69%

Evaluation of two methods to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia: A method-comparison study

Karakochuk

Janmohamed

Whitfield

et al. 2015

Clinica Chimica Acta

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 69%

Evaluation of two methods to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia: A method-comparison study

Karakochuk

Janmohamed

Whitfield

et al. 2015

Clinica Chimica Acta

View full text Add to dashboard Cite

“…10 Blood obtained via skin puncture is a mixture of blood from the arterioles, venules, and capillaries and contains traces of interstitial and intracellular fluids. 9,11 Therefore, the first drop contains more tissue fluid and should be wiped away with a sterile gauze pad. Milking or squeezing the puncture may cause hemolysis and the addition of tissue fluids.…”

Section: Blood Collectionmentioning

confidence: 99%

Dried Blood Spot Methods in Therapeutic Drug Monitoring: Methods, Assays, and Pitfalls

Edelbroek¹,

Heijden²,

Stolk³

2009

Therapeutic Drug Monitoring

461

401

View full text Add to dashboard Cite

This article reviews dried blood spot (DBS) sampling in therapeutic drug monitoring. The DBS method involves applying whole blood obtained via a fingerprick to a sampling paper. After drying and transportation, the blood spot is extracted and analyzed in the laboratory. Assays of many medicines in DBS have already been reported in the literature and are reviewed here. The technique involved in and factors that may influence the accuracy and reproducibility of DBS methods are also discussed. DBS sampling ultimately seems to be a useful technique for therapeutic drug monitoring that could have many advantages in comparison with conventional venous sampling. However, its benefits must be weighed against the degree of potential errors introduced via the sampling method; there is evidently a need for more standardization, quality assurance, basic research, and assay development.

show abstract

“…The identical offset of 4.1 mEq/L indicates that there are no intrinsic differences in potassium levels between venous and capillary samples, which is in correspondence with previously published results. 17 For LDH, no significant differences between venous and capillary samples were found (Figure, b).…”

Section: Resultsmentioning

confidence: 84%

“…This corresponds well with the general knowledge that hemolysis is a more common problem in capillary than in venous sampling. 17 The Figure (a) shows mean potassium concentrations in venous and capillary samples versus H-index. At the same H-index, capillary samples clearly show higher potassium increases than venous samples.…”

Section: Resultsmentioning

confidence: 99%

Potassium but Not Lactate Dehydrogenase Elevation Due To In Vitro Hemolysis Is Higher in Capillary Than in Venous Blood Samples

Oostendorp

Solinge

Kemperman

2012

Archives of Pathology &Amp; Laboratory Medicine

View full text Add to dashboard Cite

N Context.-Elevated potassium concentrations due to in vitro hemolysis can lead to errors in diagnoses and treatment. Recently, we observed that potassium elevation in capillary samples appeared higher than expected, based on hemolytic index (H-index).Objective.-To investigate the relation between potassium increase and H-index for capillary samples. As a control, the same analysis was performed for lactate dehydrogenase (LDH).Design.-Potassium results of 332 760 venous and 2620 capillary samples were selected. For LDH, 135 974 venous and 999 capillary samples were included. Venous and capillary samples were differentiated by using patient age, as we perform mostly capillary blood sampling in children and venous sampling in adults. Results were obtained with Beckman-Coulter DxC800 analyzers.Results.-The increase in potassium with increasing Hindex was considerably higher for capillary samples than venous samples. Linear regression revealed a potassium increase of 0.38 mEq/L per increment in H-index for capillary samples, whereas a 0.17 mEq/L increase was found for venous samples. For LDH, no differences were found between venous and capillary samples.Conclusions.-At identical H-index, capillary samples showed higher potassium elevations than venous samples. A possible explanation is that capillary sampling causes increased leakage of ions, such as potassium, from erythrocytes, compared with proteins such as hemoglobin and LDH. These results are especially important considering the increasing use of whole blood point-of-care analyzers, where the H-index is often not determined. Potassium results should therefore be interpreted with caution to avoid severe misdiagnosis of hypokalemia and hyperkalemia.

show abstract

On the composition of capillary and venous blood serum

Cited by 69 publications

References 17 publications

Evaluation of two methods to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia: A method-comparison study

Evaluation of two methods to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia: A method-comparison study

Dried Blood Spot Methods in Therapeutic Drug Monitoring: Methods, Assays, and Pitfalls

Potassium but Not Lactate Dehydrogenase Elevation Due To In Vitro Hemolysis Is Higher in Capillary Than in Venous Blood Samples

Contact Info

Product

Resources

About