On the mechanism of hydrogen evolution by the uptake hydrogenase of Bradyrhizobium japonicum

Fernández, Vı́ctor M.; Munilla, Roberto; Reyes, P.; Barate, M.; Ruiz-Argüeso, Tomás

doi:10.1016/0304-5102(89)80057-4

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Also, a strong correlation between uptake hydrogenase and nitrogenase has been previously confirmed [31]. Only one uptake hydrogenase (from B. japonicum) has been reported to have hydrogenevolving capacity, and this enzyme evolved hydrogen only under acidic conditions (pH optimum, 4.0) [38]. Therefore, we do not consider that the hydrogen recorded from Frankia sp.…”

Section: Resultsmentioning

confidence: 85%

A hydrogen-evolving enzyme is present in Frankia sp. R43

Mohapatra

Leul

Mattsson

et al. 2004

FEMS Microbiology Letters

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The ability to evolve hydrogen using methyl viologen as an electron donor was assayed in the nitrogen-fixing actinomycetes Frankia sp. R43 and Frankia sp. KB5. To further examine the nature of hydrogen-evolving enzymes that may be present in these organisms immunological studies were performed. Under anaerobic conditions (both nitrogen-limiting and nitrogen-containing) Frankia sp. R43 but not Frankia sp. KB5 evolved hydrogen,which was not linked to NAD-reducing activity. Immunological analysis of total protein from Frankia sp. R43 and Frankia sp. KB5 using an antiserum raised against Ralstonia eutropha HoxF, recognized an antigen in Frankia sp. R43 but not in Frankia sp. KB5. Immunogold labeling using antibodies raised against the R. eutropha HoxH recognized sites in both hyphae and vesicles of Frankia sp. R43, but not in Frankia sp. KB5. Based on these physiological and immunological findings, we conclude that Frankia sp. R43 has a hydrogen-evolving hydrogenase.

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Section: Resultsmentioning

confidence: 85%

A hydrogen-evolving enzyme is present in Frankia sp. R43

Mohapatra

Leul

Mattsson

et al. 2004

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…Also, a strong correlation between uptake hydrogenase and nitrogenase has been previously confirmed [31]. Only one uptake hydrogenase (from B. japonicum ) has been reported to have hydrogen‐evolving capacity, and this enzyme evolved hydrogen only under acidic conditions (pH optimum, 4.0) [38]. Therefore, we do not consider that the hydrogen recorded from Frankia sp.…”

Section: Resultsmentioning

confidence: 85%

A hydrogen-evolving enzyme is present in Frankia sp. R43

Mohapatra

Leul

Mattsson

et al. 2004

FEMS Microbiology Letters

View full text Add to dashboard Cite

The ability to evolve hydrogen using methyl viologen as an electron donor was assayed in the nitrogen‐fixing actinomycetes Frankia sp. R43 and Frankia sp. KB5. To further examine the nature of hydrogen‐evolving enzymes that may be present in these organisms immunological studies were performed. Under anaerobic conditions (both nitrogen‐limiting and nitrogen‐containing) Frankia sp. R43 but not Frankia sp. KB5 evolved hydrogen, which was not linked to NAD‐reducing activity. Immunological analysis of total protein from Frankia sp. R43 and Frankia sp. KB5 using an antiserum raised against Ralstonia eutropha HoxF, recognized an antigen in Frankia sp. R43 but not in Frankia sp. KB5. Immunogold labeling using antibodies raised against the R. eutropha HoxH recognized sites in both hyphae and vesicles of Frankia sp. R43, but not in Frankia sp. KB5. Based on these physiological and immunological findings, we conclude that Frankia sp. R43 has a hydrogen‐evolving hydrogenase.

show abstract

On the mechanism of hydrogen evolution by the uptake hydrogenase of Bradyrhizobium japonicum

Cited by 2 publications

References 17 publications

A hydrogen-evolving enzyme is present in Frankia sp. R43

A hydrogen-evolving enzyme is present in Frankia sp. R43

A hydrogen-evolving enzyme is present in Frankia sp. R43

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