One step preparation of a biocompatible, antimicrobial reduced graphene oxide–silver nanohybrid as a topical antimicrobial agent

Barua, Shaswat; Thakur, Suman; Aidew, Lipika; Buragohain, Alak Kumar; Chattopadhyay, Pronobesh; Karak, Niranjan

doi:10.1039/c3ra46835f

Cited by 72 publications

(46 citation statements)

References 23 publications

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“…7) 64 . With regard to the rGO–nAg composite, physical interaction between the sharp edges of rGO sheets disrupts the cell membrane and facilitates the transport of silver ions across the cell membrane 65–68 .…”

Section: Discussionmentioning

confidence: 99%

Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

Prasad

Lekshmi

Ostrikov³

et al. 2017

Sci Rep

162

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Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO–nAg nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely Gram-positive coccal Staphylococcus aureus and Gram-negative rod-shaped Escherichia coli and Proteus mirabilis are investigated. At the same concentration (100 µg/ml), rGO–nAg nanocomposite was significantly more effective against all three pathogens than either rGO or nAg. The nanocomposite was equally active against P. mirabilis and S. aureus as systemic antibiotic nitrofurantoin, and significantly more effective against E. coli. Importantly, the inhibition was much faster in the case of rGO–nAg nanocomposite compared to nitrofurantoin, attributed to the synergistic effects of rGO–nAg mediated contact killing and oxidative stress. This study may provide new insights for the better understanding of antibacterial actions of rGO–nAg nanocomposite and for the better designing of graphene-based antibiotics or other biomedical applications.

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Section: Discussionmentioning

confidence: 99%

Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

Prasad

Lekshmi

Ostrikov³

et al. 2017

Sci Rep

162

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“…Mirco-dilution technique was adopted for the assay. 36 S. aureus and E. coli were cultured in Nutrient Broth (NB, HiMedia, India) for 24 h at 37 °C. C. albicans was grown in Potato Dextrose Broth (PDB, HiMedia, India) for 48 h at 28 °C inside an incubator.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Self-healable castor oil based tough smart hyperbranched polyurethane nanocomposite with antimicrobial attributes

2015

Self Cite

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Here, castor oil based tough hyperbranched polyurethane/sulfur nanoparticles decorated reduced graphene oxide (HPU/SRGO) nanocomposites are fabricated with different weight% of nanohybrid. Tremendous enhancement of mechanical properties such as tensile strength (from 7.2 to 24.3 MPa), tensile modulus (from 3.3 to 137.7 MPa), toughness (from 25.4 to 313.52 MJm -3) and elongation at break (from 710 to 1456%) are observed upon incorporation of nanohybrid in HPU matrix due to str ong interaction between SRGO and HPU matrix. The nanocomposite exhibited excellent repeatable selfhealing (within 50-60s at 360W under microwave and 5-7.5 min under sunlight) and shape recovery (within 30-50s at 360W under microwave and 1-3 min under sunlight). The nanocomposite also demonstrated profound microbial inhibitory effect against Staphylococcus aureus, Escherichia coli and Candida albicans. Thus, the studied nanocomposite has tremendous potential for various advanced applications. Fig. 8 The healing efficiency of the nanocomposite at different MW power input of (a) 180 W, (b) 360 W and (c) 540 W; and (d) repres entative stress-strain curves of HPU/SRGO 2 before crack and after healing the crack.Fig. 9 MIC of nanocomposite against (a) Candida ablicans, (b) Escherichia coli, (c) Staphylococcus aureus; and SEM image of E. coli cells adhered to (d) HPU, (e) HPU/RGO and (f) HPU/SRGO.

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“…23 Then, GO sheets would provide a large surface area for bacterial attachment, thus allowing a more intimate contact between the AgNPs and the surface of bacterial cells. 19,25,[51][52][53] In our current study, the most likely antimicrobial mechanism for the GO-Ag nanocomposite might be related to the release of Ag + ions and the direct contact of bacterial cells with the AgNPs attached to the GO surface. As observed by TEM analysis, GO-Ag sheets are accumulated around the bacterial cell surfaces (Figures 5C-F and 6C-F).…”

Section: 57mentioning

confidence: 99%

Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Moraes¹,

Lima²,

Faria³

et al. 2015

IJN

121

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Background Methicillin-resistant Staphylococcus aureus (MRSA) has been responsible for serious hospital infections worldwide. Nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. In the past decade, graphene oxide (GO) has emerged as a material that is often used to support and stabilize silver nanoparticles (AgNPs) for the preparation of novel antibacterial nanocomposites. In this work, we report the synthesis of the graphene-oxide silver nanocomposite (GO-Ag) and its antibacterial activity against relevant microorganisms in medicine. Materials and methods GO-Ag nanocomposite was synthesized through the reduction of silver ions (Ag + ) by sodium citrate in an aqueous GO dispersion, and was extensively characterized using ultraviolet-visible absorption spectroscopy, X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, and transmission electron microscopy. The antibacterial activity was evaluated by microdilution assays and time-kill experiments. The morphology of bacterial cells treated with GO-Ag was investigated via transmission electron microscopy. Results AgNPs were well distributed throughout GO sheets, with an average size of 9.4±2.8 nm. The GO-Ag nanocomposite exhibited an excellent antibacterial activity against methicillin-resistant S. aureus , Acinetobacter baumannii , Enterococcus faecalis , and Escherichia coli . All (100%) MRSA cells were inactivated after 4 hours of exposure to GO-Ag sheets. In addition, no toxicity was found for either pristine GO or bare AgNPs within the tested concentration range. Transmission electronic microscopy images offered insights into how GO-Ag nanosheets interacted with bacterial cells. Conclusion Our results indicate that the GO-Ag nanocomposite is a promising antibacterial agent against common nosocomial bacteria, particularly antibiotic-resistant MRSA. Morphological injuries on MRSA cells revealed a likely loss of viability as a result of the direct contact between bacteria and the GO-Ag sheets.

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One step preparation of a biocompatible, antimicrobial reduced graphene oxide–silver nanohybrid as a topical antimicrobial agent

Cited by 72 publications

References 23 publications

Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

Self-healable castor oil based tough smart hyperbranched polyurethane nanocomposite with antimicrobial attributes

Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

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