Intraoperative ultrasound (IOUS) can provide various diagnostic information that is otherwise not available, and can guide or assist various surgical procedures in real time. With refinement of equipment, IOUS is currently used in a wide variety of surgical operations,such as hepatobiliary, pancreatic, endocrine, cardiovascular,and neurologic surgery. Our overview of IOUS, including instrumentation,techniques, indications, advantages, disadvantages,and future perspective, is described in this article. Being safe, quick, accurate, and versatile intraoperatively, IOUS is a valuable technique that surgeons are recommended to master to improve intraoperative decision making and surgical procedures.