Opine biosynthesis and catabolism genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes

Ia, Vladimirov; Matveeva, T. V.; Лутова, Л. А.

doi:10.1134/s1022795415020167

Cited by 39 publications

(23 citation statements)

References 38 publications

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“…Agrobacterium is well known for its capacity to transfer part of its DNA (T‐DNA) to plant cells. Under natural conditions these T‐DNAs induce the formation of crown gall tumors and hairy roots that synthesize small molecules used by the bacterium for its growth, the so‐called opines (Moore et al ., ; Vladimirov et al ., ). Several types of opine synthesis genes have been described.…”

Section: Introductionmentioning

confidence: 97%

Root‐specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism Nicotiana tabacum

Chen

Borne²,

Julio³

et al. 2016

The Plant Journal

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SUMMARYPrevious studies have shown that Nicotiana tabacum contains three Agrobacterium-derived T-DNA sequences inherited from its paternal ancestor Nicotiana tomentosiformis. Among these, the TB locus carries an intact mannopine synthase 2 0 gene (TB-mas2 0 ). This gene is similar to the Agrobacterium rhizogenes A4-mas2 0 gene that encodes the synthesis of the Amadori compound deoxyfructosyl-glutamine (DFG or santhopine). In this study we show that TB-mas2 0 is expressed at very low levels in N. tomentosiformis and in most N. tabacum cultivars; however, some cultivars show high TB-mas2 0 expression levels. The TB-mas2 0 promoter sequences of low-and high-expressing cultivars are identical. The low/high level of expression segregates as a single Mendelian factor in a cross between a low-and a high-expression cultivar. pTBmas2 0 -GUS and pA4-mas2 0 -GUS reporter genes were stably introduced in N. benthamiana. Both were mainly expressed in the root expansion zone and leaf vasculature. Roots of tobacco cultivars with high TBmas2 0 expression contain detectable levels of DFG.

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Section: Introductionmentioning

confidence: 97%

Root‐specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism Nicotiana tabacum

Chen

Borne²,

Julio³

et al. 2016

The Plant Journal

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“…The T‐DNA region in the pRi of the agropine‐type strains is non‐continuous and divided into two separate T‐DNA fragments, including TL (left)‐DNA, and TR (right)‐DNA, whereas other strains have an uninterrupted T‐DNA. The rol genes ( rol A, B, C, and D) responsible for hairy‐root induction are present in TL‐DNA fragment . As a natural genetic engineering agent, A. rhizogenes transfers its plasmid T‐DNA into the host plant genome and changes the gene expression resulting in modification of the metabolism of plant hormones, which eventually leads to the emergence of HRs .…”

Section: Introductionmentioning

confidence: 99%

“…The rol genes (rol A, B, C, and D) responsible for hairy-root induction are present in TL-DNA fragment. 14 As a natural genetic engineering agent, A. rhizogenes transfers its plasmid T-DNA into the host plant genome and changes the gene expression resulting in modification of the metabolism of plant hormones, which eventually leads to the emergence of HRs. 15 Numerous factors, such as bacterial strain, plant species, the culture medium composition, and the growth conditions can influence the transformation efficacy.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

et al. 2020

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BACKGROUND Ficus carica L., an ancient source of food and medicines, is rich in valuable nutritional and secondary compounds with antioxidant, antimicrobial, and anticancer effects. The present study is the first attempt to examine hairy root (HR) induction of F. carica (Sabz and Siah) by inoculating the 3‐week‐old shoots and leaves with different strains of Agrobacterium rhizogenes and also to investigate methyl jasmonate (MeJA) elicitation of HRs to produce a fast and high‐yield production method for secondary metabolites. RESULTS The maximum transformation rate (100%) was achieved by inoculating the shoots with Agrobacterium rhizogenes strain A7. Siah HRs elicited with 100 and 200 μmol L−1 MeJA and Sabz HRs with 100 μmol L−1 MeJA showed the highest total phenolic content. The highest flavonoid content was 3.935 mg QE g−1 DW in Siah HRs treated with 200 μmol L−1 MeJA and 2.762 mg QE g−1 DW in Sabz HRs treated with 300 μmol L−1 MeJA. The 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) value of HRs were affected by MeJA treatments. Methyl jasmonate elicitation also significantly enhanced the content of six phenolic acids (gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, and cinnamic acid) and three flavonoids (rutin, quercetin, and apigenin). Thymol, a monoterpene phenol, was the main HR compound detected in gas chromatography mass spectrometry (GC–MS) analysis of the essential oils. CONCLUSION Induction of HRs and elicitation of F. carica HRs by MeJA resulted in a significant increase in the production of important phenolic compounds and a significant increase in antioxidant capacity. © 2020 Society of Chemical Industry

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“…These bacteria transfer their tumor‐inducing (Ti) plasmid, which contains the genes encoding for opine synthesis, to plants cells. The latter transform in crown gall tumors and start producing opines which represent specific growth substrates for the bacteria; this is known as the “opine concept” , , . On the other hand, marine invertebrates (e.g.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Two Epimers of Pseudopaline

et al. 2020

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Opines are a known group of compounds characterized by an elevated polarity. Recently, two new members of this class, staphylopine and pseudopaline, have been identified in Staphylococcus aureus and Pseudomonas aeruginosa, respectively. These molecules are metal chelators that contribute to the growth of bacteria in particularly metal‐poor environment. Different evidences suggest that these molecules might have an important role in the development of pulmonary infections in humans. Considering the impact of P. aeruginosa infections in cystic fibrosis patients (prevalence up to 70 %), pseudopaline has risen interest as potential source of new therapeutic intervention. We present herein a straightforward synthetic approach for the synthesis of the two epimers of pseudopaline. Starting from a chiral building block, we attribute the absolute configuration to the two obtained diasteroisomers.

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Opine biosynthesis and catabolism genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes

Cited by 39 publications

References 38 publications

Root‐specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism Nicotiana tabacum

Root‐specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism Nicotiana tabacum

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

Synthesis of Two Epimers of Pseudopaline

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