Opioid activities of β-casomorphins

Brantl, Victor; Teschemacher, H.; Bläsig, J; Henschen, Agnes; Lottspeich, Friedrich

doi:10.1016/0024-3205(81)90297-6

Cited by 215 publications

(72 citation statements)

References 10 publications

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“…There are at least two observations that are relevant in this regard: 1) P-casomorphin-5 and 7 and morphiceptin (Tyr-Pro-Phe-Pro-NH2) can be cleaved from larger molecules such as BCMIR in the blood or other tissues such as brain (3), and 2) these shorter P-casomorphins are well known selective and powerful popioid agonists (4,18). Whether BCMIR itself has opioid activity is not known, but its cleavage products do (2,3).…”

Section: Discussionmentioning

confidence: 99%

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

et al. 1989

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ABSTRACT. To determine the relation between milk intake and plasma levels of P-casomorphins as a function of age, we studied the levels of P-casomorphin-7 immunoreactive material (BCMIR) in 2-and 4-wk-old beagle neonates and adult dogs following milk intake. We used three kinds of milk: 1) bovine casein-based milk formula; 2) canine bitch's milk, and 3) soy protein-based (noncasein) formula. Using a new extraction technique, BCMIR was isolated from plasma and assayed by radioimmunoassay at 0, 2, 4, and 6 h after feeding. We found that BCMIR increased significantly in both 2-and 4-wk-old puppies post bovine and canine milk feeding, but not after soy protein formula. Base-line values were about 80-120 fmoll ml and increased to 100-120% 2 h after bovine casein intake in the older puppies and by 4 h in the younger group. BCMIR levels were undetectable in adult dogs before or after bovine milk feeding. 0-casomorphin-7 added to plasma was rapidly degraded whereas BCMIR was stable. Chromatographic profiles demonstrated that the BCMIR moiety is larger than ,f3-casomorphin-7. Our data suggest that the appearance of BCMIR in the plasma of neonatal dogs is a result of P-casein degradation along a permeable gastrointestinal mucosa. We speculate that morphiceptin and P-casomorphins can be cleaved from BCMIR and may have important physiologic effects. (Pediatv Res 26: 34-38, 1989) Abbreviations BCMIR, P-casomorphin-7 immunoreactive material TFA, trifluoroacetic acid Recently, peptide fragments of p-casein, a major milk protein, have been shown to possess opioid activity (1). These peptides, known as (3-casomorphins and morphiceptin, exhibit selective popioid receptor activity like that of morphine (2, 3). For example, p-casomorphins have been shown to induce analgesia (4), influence pancreatic function ( 5 , 6), depress cardiorespiratory function (7), and alter behavior (8).Even though the mechanism of cleavage of this peptide from

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Section: Discussionmentioning

confidence: 99%

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

et al. 1989

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“…The results can be summarized as follows: (i) Proteolytic enzymes of gastrointestinal tract plus Ieucine aminopeptidase, under controlled conditions, do not release ~-casomorphin 7 from &casein but a putative precursor, i.e., procasomorphin; (ii) Procasomorphin is further digested by brush border peptidases but not to casomorphin; (iii) The peptide Tyr-Pro-Phe still resistant to proteolytic activity of the brush border is devoid of any opioid activity [9]. Apparently our results seem then to exclude a physiological role for ,&casomorphin.…”

Section: Brush Border Digestmentioning

confidence: 96%

Does casomorphin have a functional role?

Petrilli¹,

Picone²,

Caporale³

et al. 1984

FEBS Letters

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“…Sur la base du test «dit» du retrait de la queue des souris après pincement, l'injection intracérébroventriculaire des différentes j3-casomorphinesa permis de quantifier leur activité antidouleur, non seulement entre elles, mais aussi par rapport à la morphine. Par exemple, la j3-casomorphine 1-5 est de 10 à 20 fois moins active que la morphine (Brantl et al, 1981), bien qu'il suffise de 0,06 à 2 J1 mole par rat pour obtenir un effet analgé-sique appréciable; en revanche, la j3-casomorphine 1-4 NH 2 , appelée encore morphiceptine, modifiée par substitution des acinés L par position 2 et 4 par des acides aminés D ou par de l'acide D pipé-colique serait 10 fois plus active que la morphine (Chang et al, 1982). La formation au niveau de l'intestin grêle de la morphiceptine semble hautement probable, puisqu'une réaction positive est observée avec un anticorps spécifique et que des enzymes d'amidation existent dans le tractus gastro-intestinal et dans le suc pancréatique (Chang et al, 1985).…”

Section: Peptides à Activité Opiacéeunclassified

Peptides du lait à activité biologique

Maubois

Léonil

1989

Lait

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Opioid activities of β-casomorphins

Cited by 215 publications

References 10 publications

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

Does casomorphin have a functional role?

Peptides du lait à activité biologique

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