Optical Anisotropy of Supported Lipid Structures Probed by Waveguide Spectroscopy and Its Application to Study of Supported Lipid Bilayer Formation Kinetics

Mashaghi, Alireza; Swann, Marcus J.; Popplewell, Jonathan; Textor, Marcus; Reimhult, Erik

doi:10.1021/ac800988v

Cited by 46 publications

(89 citation statements)

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“…The DOPC SLB was found to be 4.6 Ϯ 0.3 nm in thickness, corresponding to a surface area per lipid molecule of 0.53 nm 2 . These data correlate well with expected values of the phosphatidylcholine headgroup, which requires a molecular area of 0.47-0.54 nm 2 (43) and with literature mass values (36). Adding DOPS gives rise to the same mass coverage and a birefringence with a similar or slightly lower value.…”

Section: Characterization Of Phospholipid Membrane Environmentssupporting

confidence: 86%

“…The measured phase change data from the two polarization modes are analyzed with the Analight DPI Explorer software which uses Maxwell's equations for the distribution of light in a waveguide to determine the refractive index and thickness of an equivalent uniform isotropic layer on top of the sensing waveguide surface. The mass per unit area of an adsorbed layer can be determined from thickness and refractive index using the Feijter formula, which uses the refractive index increments (dn/dc) of the lipid and protein, which are 0.135 and 0.182 ml/g, respectively, as reported previously (36,41). Alternatively, where the layer on the surface of the waveguide is birefringent, as is the case for SLBs, the refractive index (or thickness) of the layer can be assumed to be constant, and the thickness (or refractive index) and birefringence can be calculated (36,37).…”

Section: Methodsmentioning

confidence: 99%

“…The mass per unit area of an adsorbed layer can be determined from thickness and refractive index using the Feijter formula, which uses the refractive index increments (dn/dc) of the lipid and protein, which are 0.135 and 0.182 ml/g, respectively, as reported previously (36,41). Alternatively, where the layer on the surface of the waveguide is birefringent, as is the case for SLBs, the refractive index (or thickness) of the layer can be assumed to be constant, and the thickness (or refractive index) and birefringence can be calculated (36,37). The birefringence or optical anisotropy measures the difference in refractive index of the two orthogonal polarizations caused by the net alignment of the lipid molecules perpendicular to the waveguide surface that creates an anisotropic system.…”

Section: Methodsmentioning

confidence: 99%

“…Protein/lipid mass ratio values were calculated using the mass values of the corresponding SLBs prior to protein injection, together with the additional mass gain after protein injection. The mass changes were determined using the dn/dc values for proteins and lipids of 0.182 and 0.135 ml/g, respectively (36).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling

Ouberaï

Wang

Swann

et al. 2013

Journal of Biological Chemistry

192

156

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Background: ␣-Synuclein folds into an amphipathic ␣-helical structure upon membrane interaction. Results: The binding is promoted by lipid packing defects found in vesicles of high curvature and in planar membranes with cone-shaped lipids. Conclusion:The insertion of ␣-synuclein induces a lateral expansion of lipids that can progress to membrane remodeling. Significance: These findings support the role of ␣-synuclein in vesicle trafficking.

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Section: Characterization Of Phospholipid Membrane Environmentssupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling

Ouberaï

Wang

Swann

et al. 2013

Journal of Biological Chemistry

192

156

View full text Add to dashboard Cite

show abstract

“…DPI is one of the most powerful approaches in the variation measurement of the layer at a liquid-solid interface in terms of mass, thickness, and density [71,72]. Liang's group first utilized DPI to monitor the entire process of a toehold-mediated DNA strand displacement reaction in real time, and calculated its efficiency based on the mass changes on the surface [73].…”

Section: Snp Discrimination Using Dual-polarization Interferometry (Dpi)mentioning

confidence: 99%

Recent advances in molecular machines based on toehold‐mediated strand displacement reaction

Guo¹,

Bing²,

Xiao³

et al. 2017

Quant. Biol.

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Background: The DNA strand displacement reaction, which uses flexible and programmable DNA molecules as reaction components, is the basis of dynamic DNA nanotechnology, and has been widely used in the design of complex autonomous behaviors. Results: In this review, we first briefly introduce the concept of toehold-mediated strand displacement reaction and its kinetics regulation in pure solution. Thereafter, we review the recent progresses in DNA complex circuit, the assembly of AuNPs driven by DNA molecular machines, and the detection of single nucleotide polymorphism (SNP) using DNA toehold exchange probes in pure solution and in interface state. Lastly, the applications of toehold-mediated strand displacement in the genetic regulation and silencing through combining gene circuit with RNA interference systems are reviewed. Conclusions: The toehold-mediated strand displacement reaction makes DNA an excellent material for the fabrication of molecular machines and complex circuit, and may potentially be used in the disease diagnosis and the regulation of gene silencing in the near future.

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Capsosomes with Multilayered Subcompartments: Assembly and Loading with Hydrophobic Cargo

Hosta‐Rigau

Städler

Yan

et al. 2009

Adv Funct Materials

111

127

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Therapeutic artificial cells or organelles are nanoengineered vehicles that are expected to substitute for missing or lost cellular function. The creation of capsosomes, polymer carrier capsules containing liposomal subcompartments, is a promising approach towards constructing such therapeutic devices using the layer‐by‐layer assembly method. Herein, the assembly of intact, nonaggregated capsosomes containing multiple liposome layers is reported. It is also further demonstrated that thiocoraline, a hydrophobic model peptide with antitumor activity, can be efficiently loaded into the membrane of the liposomal subcompartments of the capsosomes. Cell viability assays verify the activity of the trapped antitumor cargo. It is also shown that pristine capsosomes do not display inherent cytotoxic effects. The ability to tune the number of liposome layers and hence the drug loading in capsosomes as well as their noncytotoxicity provide new opportunities for the creation of therapeutic artificial cells and organelles.

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Optical Anisotropy of Supported Lipid Structures Probed by Waveguide Spectroscopy and Its Application to Study of Supported Lipid Bilayer Formation Kinetics

Cited by 46 publications

References 0 publications

α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling

α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling

Recent advances in molecular machines based on toehold‐mediated strand displacement reaction

Capsosomes with Multilayered Subcompartments: Assembly and Loading with Hydrophobic Cargo

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