Optical isomer separation of flavanones and flavanone glycosides by nano-liquid chromatography using a phenyl-carbamate-propyl-β-cyclodextrin chiral stationary phase

Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography

“…The optimization of the chiral separation of hesperetin in terms of resolution and selectivity using different chromatographic conditions was already done in a previous work [19]. The best separation of HT enantiomers and IS was achieved using a mobile phase based on a mixture of 1% (v/v) (TEAA) pH 4.5 in 30/70 (v/v) water/methanol.…”

“…Yáñez et al developed a HPLC method for the separation of these chiral compounds using a commercial cellulose based silica column [3]. Recently, racemic hesperetin was resolved in its enantiomers by our group using nano-liquid chromatography (nano-LC) by means of a phenyl-carbamate-propyl-␤-cyclodextrin stationary phase [19].…”

Cyclodextrin‐Functionalized Monolithic Capillary Columns: Preparation and Chiral Applications

“…After that, amphiphilic stationary phases were prepared through the copolymerization of the obtained bisacrylamide‐CD host–guest complexes with hydrophilic monomers and an additional hydrophilic crosslinker in aqueous solution. The complexes formation, as well as the formation of the polyrotaxane architecture, were confirmed by H‐NMR, solid state C‐NMR spectroscopy, and CD‐modified micellar electrokinetic chromatography. With these capillaries, sufficiently high electroosmotic flow velocity, high reproducibility, and repeatability of separation parameters and high theoretical plate numbers of up to 200,000 m ‐1 were obtained.…”

“…The need for faster and more efficient analytical systems with minimal solvent and sample consumption triggered the emergence of capillary liquid chromatography (CLC) and capillary electrochromatography (CEC). Yet chromatographic separations can be performed in columns with minuscule internal diameters (up to 300 µm) . This enabled the use of very low mobile phase flow rates (up to 1000 nL/min) and faster separations to be achieved.…”

Enantiomeric separations by means of nano‐LC

“…A CSP based on β‐CD was also employed by Si‐Ahmed et al in nano‐LC to perform the enantioseparation of several flavanoids and diastereoisomeric separation of two flavanone glycosides . Good eanantioresolutions were achieved, in shorter analysis time compared to the results reported in literature, for the same compounds, with conventional HPLC.…”