Optical plankton analyser: A flow cytometer for plankton analysis, II: Specifications

Dubelaar, G. B. J.; Groenewegen, Ad C.; Stokdijk, Willem; Engh, G.J. van den; Visser, J. W. M.

doi:10.1002/cyto.990100508

Cited by 74 publications

(46 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…That there are fewer large cells than small cells means a greater volume of sample is required to encounter a (statistically) reasonable number of large cells. Although flow cytometers can be made (Dubelaar et al 1989) or modified (Olson et al 1993) to achieve high sample throughout, most only operate at -1 ~1 s--l or less. In the present study, a sample volume of no more than 1 ml was analyzed in each case.…”

Section: Discussionmentioning

confidence: 99%

Cytometric diversity in marine ultraphytoplankton

1997

Limnology & Oceanography

View full text Add to dashboard Cite

The concept and methods of ecological diversity in communities were applied to phytoplankton categorized by flow cytometric measurements related to size and chlorophyll content. Each cytometric signature was condensed to single numerical values indicative of diversity and evenness. Measurements pooled from studies disparate in temporal and spatial scales indicated greater chlorophyll biomass and primary production with greater cytometric diversity and evenness. Future development of these ideas may help link biological oceanographic processes with patterns established through ecological processes at the community level.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytometric diversity in marine ultraphytoplankton

1997

Limnology & Oceanography

View full text Add to dashboard Cite

show abstract

“…ment of Aquatic Ecology, University of Amsterdam) . This flowcytometer was specially designed for phytoplankton counting (Dubelaar et al ., 1989) . On basis of the optical characteristics of the phytoplankton, a distinction can be made between different plankton groups (Balfoort et al ., 1992) .…”

Section: Biomass Determinationmentioning

confidence: 99%

Diurnal buoyancy changes of Microcystis in an artificially mixed storage reservoir

Visser

Ketelaars²,

Breemen³

et al. 1996

Hydrobiologia

View full text Add to dashboard Cite

General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Key words : buoyancy, artificial mixing, aeration, Microcystis, cyanobacteria Abstract In a storage reservoir, which is artificially mixed in order to reduce algal and especially cyanobacterial growth, the cyanobacterium Microcystis is still present . The aim of the research was to investigate why Microcystis was able to grow in the artificially mixed reservoir . From the results it could be concluded that the large shallow area in the reservoir allows this growth . The loss of buoyancy during the day was much higher in this shallow part than in the deep part. Assuming that the loss of buoyancy was the result of a higher carbohydrate content, a higher growth rate in the shallow part may be expected . A higher received light dose by the phytoplankton in the shallow mixed part of the reservoir than in the deep mixed part explains the difference in buoyancy loss . A significant correlation between the received light dose (calculated for homogeneously mixed phytoplankton) and the buoyancy loss was found . Apparently, the Microcystis colonies were entrained in the turbulent flow in both the shallow and the deep part of the reservoir. With a little higher stability on one sampling day, due to the late start of the artificial mixing, the loss of buoyancy at the deep site was higher than on the other days and almost comparable to the loss at the shallow site . Although the vertical biomass distribution and the temperature profiles showed homogeneous mixing, the colonies in the upper layers apparently received a higher light dose than those deeper in the water column . Determination of the buoyancy state of cyanobacteria appeared to be a valuable method to investigate the light history and hence their entrainment in the turbulent flow in the water column .

show abstract

“…However, coastal and inland species are not only highly heterogeneous in taxonomic composition, size, concentration, and morphology, but many also form colonies. Therefore, an optical plankton analyser (OPA; Peeters et al, 1989;Dubelaar et al, 1989) and later a more compact version, the European optical plankton analyser (EurOPA; Jonker et al, 1995) was designed and built.…”

Section: Flow Cytometry and The European Optical Plankton Analysermentioning

confidence: 99%

Immuno flow cytometry in marine phytoplankton research

Peperzak¹,

Vrieling²,

Sandee³

et al. 2000

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: The developments in the combination of flow cytometry and immunology as a tool to identify, count and examine marine phytoplankton cells are reviewed. The concepts of immunology and flow cytometry are described. A distinction is made between quantitative and qualitative immunofluorescence. Quantitative immunofluorescence, the identification and enumeration of phytoplankton cells, is the research area that has advanced rapidly in the past decade, and is reviewed extensively. Key steps of quantitative immunofluorescence, fixation and immunolabel intensity, are discussed in more detail. Qualitative immunofluorescence is a new, hardly explored but highly interesting development in which qualitative -physiological-variables related to e.g. nutrient limitation or primary production are measured in individual cells instead of phytoplankton populations as a whole. Several combinations of immunological probes, both for species identification and for physiological measurements, are proposed. A special case of qualitative immunofluorescence is the measurement of phytoplankton toxins in single cells from natural populations. It is anticipated that the future use of semiconductor nanocrystals or "quantum dots" as fluorophores will greatly enhance signal detection in flow cytometry, and hence in both quantitative and qualitative immunofluorescence applications.

show abstract

Optical plankton analyser: A flow cytometer for plankton analysis, II: Specifications

Cited by 74 publications

References 4 publications

Cytometric diversity in marine ultraphytoplankton

Cytometric diversity in marine ultraphytoplankton

Diurnal buoyancy changes of Microcystis in an artificially mixed storage reservoir

Immuno flow cytometry in marine phytoplankton research

Contact Info

Product

Resources

About